Aquistain















The AquiStain’s fluorescent dye is the result of an innovative technology and a know-how patented.
This is a new generation of fluorescent protein stain, non toxic, and more sensitive developed for identifying and quantifying low-abundance proteins.

Technology:

AquiStain’s dyes are an eco-friendly fluorescent protein stain that covalently and reversibly bind to lysine, arginine and histidine residues in proteins and peptides to yield an intensely red fluorescent product. This unique mechanism provides sensitive quantification of proteins in biological media as well as unparalleled compatibility with downstream analysis including mass spectrometry, Edman sequencing and functional analysis.
This technology have a great interest in proteomics and more generally in the field of life sciences.
The fluorescent labels of AquiStain are synthetic analogues of epicocconone, small naturally occurring fluorescent compound, marketed by Fluorotechnics as LavaPurple ™ or DeepPurple. ™
This technology is of great interest in the field of life sciences. Indeed, fluorescent compounds are widely used for diagnostic purposes, for demonstrating enzymatic activity and for visualizing proteins in biological media or in aqueous solutions, such as the 1D and 2D gel electrophoresis.
Just as epicocconone, our fluorescent labels AquiStain reversibly bind to lysine, arginine and histidine residues in proteins and peptides to yield an intensely red fluorescent product for identifying and quantifying these residues.
With its efficient synthesis process, AquiStain quickly develops fluorescent labels with high purity in a sustainable way.
 

AquiStain’s labels properties:

• Very sensitive, superior efficiency to Sypro® Ruby or silver staining.
• Ideal for multiplexing with other fluorophores
• A very large Stocks shift of about 100 nm, a small size and low molecular weight
• A good solubility in both water and in lipid bi-layers
• For use in living systems to measure dynamic processes in real time
• Compatible with standard instrumentation for fluorescence
• Environmentally friendly, heavy metal free and non-toxic.
Publications:
The AquiStain fluorescent labels are the result of innovative technology and know-how patented.
The different studies of these compounds have already resulted in the publication of many scientific articles.
 

Aquistain Red Fluorescent Total Protein Stain:

AquiStain RED dye is the most sensitive fluorescent staining available for the detection of total proteins separated on 1D and 2D gel electrophoresis, PVDF and nitrocellulose membranes and is fully compatible with mass spectrometry.
AquiStain RED is a unique fluorescent marker. This fluorophore covalently binds to proteins and is completely pH-reversible. This property makes it a very effective stain and fully compatible with downstream analysis such as mass spectrometry, Edman sequencing and functional analysis.
Thanks to its high stability, the stained gels with AquiStain RED result in low background and no speckles.
AquiStain RED Fluorescent Total Protein Stain is particularly suitable for all gel and blot staining applications. In addition, staining proteomic gels with this new fluorophore showed significant decrease lowering of the detection limit and a 30% increase in the number of low abundance proteins detected. AquiStain RED has also proved to be much more photostable with a half-life of 33 min on a UV transilluminator.
For Blot Staining, AquiStain RED is the most sensitive and simple to use fluorescent protein stain for nitrocellulose and PVDF membranes.
This reversible dye can be simply removed by washing. Then proteins may be analysed by mass spectrometry, immuno-staining and / or functional analysis.
Finally, unlike competitors products, AquiStain RED contains no heavy metals, is environmentally friendly and easy to use.
 
• Flexible: 1D or 2D electrophoresis gels, native or denatured; nitrocellulose and PVDF membranes.
• Eco-friendly: nontoxic dye, heavy metals free
• Sensitive: up to 50 pg / spot of proteins detected
• Compatible: with mass spectrometry, DIGE, Blot and phosphoprotein
• Wide dynamic range (over 4-orders of magnitude)
• Low background
• Multiplex compatible: with other fluorophores (e.g. Cy™ Dyes), other stains (e.g. Coomassie®, silver).


AquiPEP Fluorescent Protein Assay:

AquiPEP Protein & Peptide Quantification Kit is designed for accurate and highly sensitive quantification of proteins.
This assay doesn’t precipitated or denatured peptides, samples can therefore be reused. AquiPEP is significantly more sensitive and reliable than existing standard colorimetric assays and this especially for difficult to measure proteins.
AquiPEP Protein & Peptide Quantification offers many advantages compared to other peptides quantification methods.
Indeed, the dye reversibly binds to Lysines, arginine and histidine residues of peptides and proteins, generating an intense red fluorescence.
A high sensitivity is obtained, with a wide dynamic range.
AquiPEP Protein & Peptide Quantification is robust to substances that inhibit most peptide assays and is particularly suited to quantifying peptides in samples prepared for chromatography 1-D and 2-D electrophoresis, IEF gels and anywhere accurate quantification of peptides is needed.
In addition this assay is economical and suitable for many applications such as mass spectrometry, HPLC, N-terminal sequencing, DIGE …
AquiPEP Protein & Peptide Quantification is environmentally friendly, non-toxic, safe and easy to remove.
 
• Very sensitive: 100 ng/mL (peptides) and 40 ng/mL (proteins)
• Low peptide-peptide or protein-protein variability, improving accuracy for complex samples
• Wide dynamic range, over 3-orders of magnitude
• Robust to interfering compounds: such as DNA, Detergents, DTT and Urea
• Safe and environmentally friendly: non-toxic, heavy metal free
• Easy to use and quick: 60 minutes, no heating and no reduction steps
• Designed for use in a wide range of fluorescence measuring instruments
 

Live Cell Imaging Stains:

AquiCell Live Cell Imaging Stain is a fluorescent marker with a low molecular weight and small size. This fluorophore is non-toxic, cell-permeable and can be excited by several laser sources. Moreover, AquiCELL binds covalently and reversibly to primary amines, permitting detection and analysis of biomolecules.