Cellcrusher


 

The Cellcrusher reduces these tissues to a fine, easily recoverable powder.  The curved surfaces of the Cellcrusher are designed for quick, effective cleaning and decontamination between samples.

The Cellcrusher is a stainless steel device for cell disruption in liquid nitrogen.  Frozen samples are placed in the chilled Cellcrusher and pulverised by striking the device with a mallet.  The curved surfaces of the Cellcrusher facilitate effective sample retrieval by means of a contoured recovery spoon. These surfaces also allow cold, dry cleaning, saving time and increasing throughput.
 



Cell disruption background

The nature of the tissue to be processed dictates the cell disruption method to be used. Mammalian cells grown in cell culture can be disrupted by suspension in hypotonic or detergent solutions.  For most other tissues, cell disruption requires various forms of tissue grinder.  Mild approaches include use of Potter-Elvehjem tissue grinder or Dounce homogeniser.  If this type of tissue grinder is not suitable, more rigorous cell disruption methods are used, including motorised tissue grinders, bead mills and tissue pulverisers used in liquid nitrogen.
 


 

Comparison with conventional tissue pulveriser

 

Cell Crusher System

1. Liquid N2 -196oC<
2. Strike with mallet
3. The differing radii of the parts allow the sample to move laterally
4. Rearrange semi-crushed sample with spoon
5. Strike again to obtain a uniform powder

Conventional tissue pulveriser (not offered)

1. liquid N2 -196oC
2. Strike
3. The general result is a compacted lump of powder and larger chunks.
4. Due to the design of conventional tissue pulverisers, this mixture can not be rearranged and crushed again to obtain a uniform powder.

Fine, uniform powder

The Cellcrusher design allows for rearrangement and recrushing of the sample, leading to more effective cell disruption.

Curved surfaces for fast cleaning

The Cellcrusher is cleaned cold and dry, using the recovery spoon wrapped in tissue paper. This avoids the tedious task of warming and then washing an extremely cold metal object in water. Cold cleaning is not possible with a conventional tissue pulveriser, due to right-angled surfaces and narrow tubes.
 



Products

Dril Bit 

More information available here 

The Cellcrusher

​More information available here 
 

 


 

Low temperature tissue lysis using the Cellcrusher

Cryogenic tissue pulverisers are used to disrupt frozen tissues with a strong extracellular matrix, such as tumours, seeds or venous tissues. The purpose of this disruption is protein extraction from tissue. When such samples are placed in liquid nitrogen, the water in the samples freezes and becomes extremely brittle. If struck sharply, these frozen samples can shatter into a collection of very fine fragments.

However, without a frozen tissue pulveriser to contain the fragments, and maintain them at a low enough temperature to avoid melting, this method of tissue lysis is of limited utility. Using a mortar and pestle with liquid nitrogen was time consuming, laborious and wasted a lot of valuable sample, such as tumours or biopsies. The Cellcrusher tissue pulveriser’s curved surfaces facilitate easy sample recovery and cleaning, while the ergonomic design of the device, and the accompanying accessories allow for easy manipulation of the device at low temperatures. Previously, cell disruption with other pulverizers required use of awkward freezer gloves and loss of valuable sample, due to poor design.

CellCrusher also offer a drill bit accessory which is used with the tissue pulveriser and a household drill. This accessory is employed when difficult tissue lysis tasks such as zebrafish embryo lysis, seed disruption or yeast lysis are necessary. Additionally, the Cellcrusher can be cleaned at liquid nitrogen temperatures, saving the time and effort needed to warm, clean and subsequently re-cool an extremely cold metal object. Therefore, we are confident that the Cellcrusher is the best cryogenic tissue pulveriser on the market.

Cellcrusher also offer a range of gradient mixers. This gradient mixer range features innovative designs that will facilitate the production of linear, non-linear and exponential gradients.