The Cellcrusher reduces these tissues to a fine, easily recoverable powder. The curved surfaces of the Cellcrusher are designed for quick, effective cleaning and decontamination between samples.
The Cellcrusher is a stainless steel device for cell disruption in liquid nitrogen. Frozen samples are placed in the chilled Cellcrusher and pulverised by striking the device with a mallet. The curved surfaces of the Cellcrusher facilitate effective sample retrieval by means of a contoured recovery spoon. These surfaces also allow cold, dry cleaning, saving time and increasing throughput.
Cell disruption background
The nature of the tissue to be processed dictates the cell disruption method to be used. Mammalian cells grown in cell culture can be disrupted by suspension in hypotonic or detergent solutions. For most other tissues, cell disruption requires various forms of tissue grinder. Mild approaches include use of Potter-Elvehjem tissue grinder or Dounce homogeniser. If this type of tissue grinder is not suitable, more rigorous cell disruption methods are used, including motorised tissue grinders, bead mills and tissue pulverisers used in liquid nitrogen.
Comparison with conventional tissue pulveriser
Cell Crusher System
1. Liquid N2 -196oC<
2. Strike with mallet
3. The differing radii of the parts allow the sample to move laterally
4. Rearrange semi-crushed sample with spoon
5. Strike again to obtain a uniform powder
Conventional tissue pulveriser (not offered)
1. liquid N2 -196oC
3. The general result is a compacted lump of powder and larger chunks.
4. Due to the design of conventional tissue pulverisers, this mixture can not be rearranged and crushed again to obtain a uniform powder.
Fine, uniform powder
The Cellcrusher design allows for rearrangement and recrushing of the sample, leading to more effective cell disruption.
Curved surfaces for fast cleaning
The Cellcrusher is cleaned cold and dry, using the recovery spoon wrapped in tissue paper. This avoids the tedious task of warming and then washing an extremely cold metal object in water. Cold cleaning is not possible with a conventional tissue pulveriser, due to right-angled surfaces and narrow tubes.