What is Chromatrap®?
Chromatrap® is a solid-state filter-based technology that significantly enhances and accelerates the important epigenetic research tool known as ChIP.
Compared to standard methods, Chromatrap® is:
- More robust
- Easier to use
- More sensitive, especially for low-abundant targets
What is ChIP?
ChIP is a technique used to study the association of specific proteins, or their modified isoforms, with defined genomic regions. It is a fast growing research technique and is commonly used for mapping the DNA-protein interactions in cells which are crucial for correct gene regulation. For example, they may be used to determine whether proteins such as transcription factors and modified histones bind to a particular region of DNA of living cells or tissues.
In a ChIP assay, fragments of the DNA-protein complex (chromatin) are cross-linked in such a way so as to retain the specific DNA-protein interactions. The chromatin is then extracted and sheared either by sonication or enzymatic digestion into small fragments. The DNA/protein fragments are selectively immunoprecipitated using antibodies directed against the protein of interest and the resulting fractions treated to separate the DNA and protein components.
Why is the Chromatrap® technology better?
Chromatrap® kits use spin columns or filter-bottom microplates which contain discs of a novel, inert, porous polymer to which Protein A or G has been covalently attached. This patented format is unique.
During an assay, the chromatin/antibody complex is selectively retained by the disc. Washing with three buffers and an elution step are all that is required to obtain the selectively enriched DNA, making Chromatrap® more efficient in the laboratory.
Advantages of Chromatrap®:
- ChIP in under 5 hours from chromatin preparation to qPCR
- Less manual handling, eliminating sample loss through multiple pipetting steps
- Single column and 96-well high throughput formats available
- Optimised for 1000 ng sample sizes, as smaller samples give better qPCR results
- Allows more IP assays from a single sample
How does it perform in the laboratory?
Chromatrap® outperforms other chromatin immunoprecipitation assays because it has:
- Robust signal to noise- typically 2-3 times better than standard methods
- Low non-specific binding on the inert Chromatrap® discs
- No DNA clean-up required for qPCR
- High surface area and molecular mixing ideal for low abundant targets and challenging cell types
- Wide dynamic range makes it suitable for ChIP-seq
- 96 well format for high throughput analysis
- Allows more ChIP assays to be performed from a single sample