Elabscience has collected a detailed list of over 8,000 alternative Santa Cruz antibodies to secure your supply of antibodies and continuation of research.

If you cannot find your antibody on the list below please contact our technical team technical@stratech.co.uk who can help!

The Elabscience^® Protocols consist of collection of guidelines and frequently asked questions about the most commonly used immunology experiments. We built this forum to create a platform that can be used as reference for practical scientific experiments. We will continue to update and provide more technical information and practical resources.

Instrumentation sample preparation and storage for ELISA assays

How to perform an ELISA assay as perfectly as possible?

Gene synthesis, sometimes known as DNA printing, is a method in synthetic biology that is used to create artificial genes in the laboratory. Based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that it does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size.

Advantages

•  Short turn-around time.
•  100% sequence accuracy guarantee.
•  Site directed mutagenesis service.
•  Gene Synthesis Service covering any Length or Complexity
•  Free codon optimization and free cloning into many standard vectors.
•  Over 95% success rate with “No Gene, No Charge” policy. No hidden costs and no setup charges.

Price and delivery time

Note: Genes ≤8000 bp will be delivered dry in a circularized plasmid and normalized to 4 µg final yield.* For genes ˃8000 bp, please contact us.

Elabscience® provides peptide synthesis service of different quality and purity range. The quality can be from mg, g to kg. The purity covers crude, desalted, 70%, 75%，80%，85%，90%，95% and 98%.

Advantages

• Up to 100aa peptide sequences can be synthesized
•  Variety of peptide modification services
•  Over 95% of synthesis success rate
•  Fastest peptide synthesis speed in the industry
•  All peptides are analyzed by MS and HPLC

Modifications

Elabscience offer a wide range of modifications to meet any research need.

•  N-Terminal Modifications
•  C-Terminal Modifications
•  D-Amino Acids
•  Unnatural/Unusual amino acids
•  Fluorescence/Dye Labeling
•  Stable isotope labeled peptides
•  Pegylation
•  Cyclic peptide
•  Peptide conjugates

*Please contact us for the prices of different modifications.

Applications

•  Antibody epitope mapping
•  Monoclonal and polyclonal antibody production
•  Non-quantitative enzyme-substrate studies
•  Biological effects of defined peptides
•  Blocking and competition assays of proteases
•  New techniques for separation by chromatography/electrophoresis
•  Immunization and quantitative receptor-ligand interactions
•  Antibody-antigen interactions
•  ELISA standards for measuring titers of antibodies
•  Epitope mapping Structure, dynamics, and folding of peptides and proteins via NMR

Elabscience’s custom recombinant protein services provide a comprehensive portfolio of protein expression systems, including bacteria, yeast, baculovirus infected insect cells, and mammalian cells. We offer the ideal level of protein purity for your downstream applications. With customized solutions, flexible production scale, and proprietary technologies, we work in partnership with our clients to ensure efficient and high quality reagent protein production, process development and scale-up production of biologics for research purposes.

Elabscience^® has delivered over 2,000 batches of recombinant proteins used for functional assays, high throughput screens, antigen generation, structural analysis, and many other drug discovery applications. When our extensive expertise in recombinant proteins is integrated with our in-house gene synthesis and assay development capabilities, we can quickly deliver your projects in a flexible and cost-effective manner. Moreover, our dedicated technical account managers will guide your project through every step of the process, and constantly keep you informed of the project progress.

Advantages

•  Comprehensive expression systems: Bacteria, yeast, baculovirus/insect cell, and mammalian cell with various vectors for each system.
•  Fast turn-around: 10 mg of purified protein within 5 weeks merely from you submitted your target gene sequence.
•  One-stop service platform: Elabscience^® can take your project directly from gene synthesis, to any of those further stages including recombinant protein expression, antibody production, and assay development.

Available Protein Services

•  Yeast Expression System
  Proprietary Technologies / Humanized antibody production / Streamlined Services
•  Bacterial Expression System
  Competitive price / Fast turn-around / Flexible scale-up protein production / Guaranteed Package

Unlike monoclonal antibodies, polyclonal antibodies correspond to multiple epitopes and tend to contain several immunoglobulin subtypes. They can be used as both primary and secondary antibodies in Westerns, ELISAs, and IHCs.

Elabscience^® provides polyclonal antibody services in mouse, rat, rabbit, and other species. Our polyclonal antibody packages include antigen design, peptide synthesis, immunization, purification, ELISA test, and lyophilization. You can simply select your desired packages and send us the specific requirements, and then Elabscience^® will offer antibodies with guaranteed quality.

Advantages

•  Multiple antibody labeling for alternatives: Elabscience^® offers multiple antibody labeling for you to choose, including Biotin, HRP, FITC, Cy3, Cy5, APC, etc.
•  Flexible service portfolio: Different host species (mouse, rat, and rabbit) and purification method (Protein A/G purification/ Antigen Affinity purification) are available for your research demands.
•  Personalized experiment scheme: Elabscience’s experienced antibody experts will design the personalized experiment scheme according to your specific requirements and applications.

Elabscience^® Super Plus High Sensitive and Rapid Immunohistochemical Kit is a high sensitive and rapid immunohistochemical broad spectrum detection reagent. There is no need to do the traditional three times of xylene dewaxing, three to five times of gradient ethanol hydration or antigen repair but only one Dewaxing/Antigen Retrieval Buffer heating without fume hood or traditional dewaxing/antigen repair tanks. The operation time is effectively shortened, the cumbersome operation steps are simplified so the variables in operation are reduced, and the stability is improved. The Dewaxing/Antigen Retrieval Buffer applies new environmental protection technology is not only reduces the harm to the body, but also cause no pollution to the environment.

This kit is easy to operate and fast because of the one-stop reagents used in the immunohistochemical experiment, and the high sensitive secondary antibody/ DAB detection reagents make this kit to be more sensitive than other produce.

This kit can be used to detect monoclonal/polyclonal antibody derived from Rabbit and Mouse.

Components:

Storage:

Store at 2~8°C, away from light. Avoid of freezing. Valid for 12 months. The reagents are valid within 6 months after opening.

• Save 1h at least
• More simplified assay procedures
• Higher stability, easier storage
• More cost-effective, lower price

Renal Function Biomarkers
TF, NGAL, β2-MG, MAU, RBP4, Cys-C, CLU

Inflammation Biomarkers
IgG, CRP, TGF-α, PCT, ALB, Acrp30, IgA, IgE, IgM, TG, IgG (Mouse)

Signal Transduction
VEGF-A, TGF-α, Acrp30

Elabscience^® has developed a series of metabolism assay kits with simple operation, high sensitivity and excellent quality through the method of enzyme reaction with fluorescent probe, allowing you to obtain accurate results in less time and samples. Elabscience^® fluorometric assay kits have applications in Oxidative stress, Liver biomarkers and Kidney biomarkers, Glycolysis, Lipids metabolism, etc. Fluorometric assay kits are suitable for a variety of sample types, involving a variety of body fluids, tissue and cells. The main fluorometric detection instrument is the fluorescence microplate reader. Fluorometric assay kits can help you to upgrade your metabolism assay experiments!

Cost-effective

Provide dilution factor of the sample

Improve experimental efficiency, Fewer experiments ）

At present, more than 30 antibodies or antibody derivatives have been approved for cancer treatment by FDA, and hundreds of antibodies are in the process of clinical trials. Antibodies have become a new choice for cancer treatment. Anti-cancer antibodies can target a wide range of antigens, including soluble proteins, cancer cell surface antigens and effector cell receptors.

The versatility of antibodies enables antibodies to take a variety of mechanisms, including direct blocking of growth factors, regulation of the immune system, and interruption or induction of signal transduction pathways. In addition, antibodies are increasingly used as carriers to improve the specificity of specific chemotherapeutic drugs.

• High antigen purity:The purity of each recombinant protein used as an immunogen is ≥90%, part of which are ≥ 95%.
• High antibody specificity:Antibodies with high affinity antigen specificity were obtained by antigen affinity purification.
• Full and accurate data of cancer antibodies:WB experimental operation guidelines include all the experimental conditions in the WB experimental process. The products have been strictly verified and the datas are accurate and reliable.
• Comprehensive and accurate experimental data:Multi species and applications quality detection to ensure that each antibody is a high-quality product that has been strictly screened.

POSTN—Periostin

POSTN is an extracellular matrix periosteal protein, which can support the growth of transferase in the formed microenvironment by enhancing Wnt signaling in tumor cells. In adults, it is expressed in specific organs such as breast, bone, skin and intestine.

CK-18– Cytokeratin 18

CK18 is a normal cytoskeleton component of hepatocytes and a standard of tumor markers. CK18 levels were increased in patients with benign liver disease, especially those with significant cytolysis.

IL-6R– Interleukin-6 receptor

IL-6R can specifically combine with IL-6 to block the related diseases caused by IL-6 and reduce the severity of asthma, multiple sclerosis, chronic rheumatoid arthritis and other diseases.

Elabscience® offers you a highly sensitive, fast and simple TUNEL apoptosis assay kit with one-step dewaxing solution, which can better assist in apoptosis research on diseases related to cell function and R&D of related drugs. TUNEL apoptosis assay kits detect the DNA breaks formed when DNA fragmentation occurs in the last phase of apoptosis, monitor and quantify apoptosis.

One-step TUNEL assay kit (FITC, AF488, AF594, AF647, AF555) is suitable for in situ apoptosis detection of tissue samples (Paraffin embedding, frozen section) and cells (Cell smears, cell climbing smears) in situ apoptotic detection. The test results can be directly observed through a fluorescence microscope.”

One step dewaxing saves about 2h, safe, non-toxic and environment friendly.

Traditional dewaxing methods
Green fluorescence
(Illumination value: 10, exposure time: 100 ms)

Comparison of traditional and one-step dewaxing
Green fluorescence
(Illumination value: 10, exposure time: 30 ms)

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. After extraction and loading for electrophoresis, a DNA ladder of 180-200 bp can be found in such DNA samples, which is a specific event during cell apoptosis. The exposed 3′-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with labeled dUTP, which can be detected with fluorescence microscopy or flow cytometry.。

Functional study of cell apoptosis

• The effect of gene up-regulation or down-regulation on cell function
• The effect of drugs on the function of cancer cells
• The effect of viruses on cell function

Research on apoptosis-related diseases

• Insufficient apoptosis: tumors, autoimmune diseases, viral infections
• Excessive apoptosis: cardiovascular disease, blood system disease, severe hepatitis, degenerative neurological disease

Formalin-fixed, paraffin embedded (FFPE) mouse enteritis tissue were stained with Elabscience^® One-step TUNEL Assay Kit (code – E-CK-A321)  (Green, AF488) [left] or other commercial TUNEL Assay Kit (Green) [right]

Mixed samples of normal mouse spleen cells and DNase I treated mouse spleen cells were stained with Elabscience^® One-step TUNEL Assay Kit (cat no. E-CK-A321) (Green, AF488) [empty black histogram] or other commercial TUNEL Assay Kit (Green) [filled gray histogram]

After more than ten years of dedicated research and development, Elabscience^® can provide you with Western Blot, IHC, IF, ELISA and other immunological reagents, to provide you with a complete set of solutions, one-stop related reagents, to meet your various laboratory needs.

FCM Related Reagents

ELISA related reagents

Labeling Kits

WB Related Reagents

IHC Related Reagents

IF Related Reagents

• Western Blot Troubleshooting Tips
• Immunohistochemistry Troubleshooting Tips
• Immunofluorescence Troubleshooting Tips
• Flow Cytometry Troubleshooting Tips
• Test Principles and Troubleshooting Tips for ELISA Assay

Fig.1 The electron microscopic (EM) image shows mature(oval-shaped) and immature(crescents and spherical ) monkeypox virus particles

Fig.2 The transmission electron microscope (TEM) image shows monkeypox virus particles

Fig.3 The DNA structure of monkeypox virus

Monkeypox Introduction

Source and Characteristic

Monkeypox virus (MPV) is an orthopoxvirus that causes a disease with symptoms similar, but less severe to smallpox. While smallpox was eradicated in 1980, monkeypox continues to occur in countries of central and west Africa. Two distinct clade are identified: the west African clade and the Congo Basin clade, also known as the central African clade. In May 2022, multiple cases of monkeypox were detected in several non-endemic countries, making it urgent to further understand the epidemiology, source of infection and mode of transmission of this disease.

Fig. Monkeypox epidemic map (Source: World Health Organization; May 21)

Monkeypox virus (MPV) is a double-stranded DNA virus belonging to orthopoxviruses of the Poxviridae family. It is one of the human orthopoxviruses, including variola (VACV), vaccinia (CPX) and vaccinia (VACV) viruses. Monkeypox virus is about 200-400 nm in size, brick or oval in shape, with stable DNA structure, which can repair itself when mutation occurs.

Transmission and Pathogenesis

Monkeypox is a zoonosis: a disease that is transmitted from animals to humans. Cases are often found close to tropical rainforests where there are animals that carry the virus. Evidence of monkeypox virus infection has been found in animals including squirrels, Gambian poached rats, dormice, different species of monkeys and others.

Human-to-human transmission is limited, with the longest documented chain of transmission being 6 generations, meaning that the last person to be infected in this chain was 6 links away from the original sick person. It can be transmitted through contact with bodily fluids, lesions on the skin or on internal mucosal surfaces, such as in the mouth or throat, respiratory droplets and contaminated objects.

Monkeypox virus invades human body from respiratory mucosa, multiplies in lymphocytes and invades blood stream to produce transient viremia. After intracellular reproduction, the cells invade the blood stream and run to the whole body skin reproduction, causing lesions.

Symptom

The incubation period of monkeypox can range from 5 to 21 days. The febrile stage of illness usually lasts 1 to 3 days with symptoms including fever, intense headache, lymphadenopathy (swelling of the lymph nodes), back pain, myalgia (muscle ache), and an intense asthenia (lack of energy). The febrile stage is followed by the skin eruption stage, lasting for 2 to 4 weeks. Lesions evolve from macules (lesions with a flat base) to papules (raised firm painful lesions) to vesicles (filled with clear fluid) to pustules (filled with pus), followed by scabs or crusts.

The disease is self-limiting, with symptoms usually lasting 2 to 4 weeks and going away without treatment, the proportion of patients who die has varied between 0 and 11% in documented cases. The main difference between the symptoms of smallpox and monkeypox is that monkeypox causes lymph nodes to swell, whereas smallpox does not.

Monkeypox Detection Method

1. What is Lateral flow assay?

The lateral flow assay kit is a chromatographic immunoassay for the qualitative detection of Ag/Ab. The test method is based on antigen-antibody. It has two letters which are test (“T”) line and control (“C”) line on the surface of device. Test line and control line in the result window are not visible before applying any samples. This visual evaluation enables qualitative analysis. This rapid detection card is easy to operate and cost-effective.

A Rapid Chromatographic Immunoassay Kit for Monkeypox Virus-Specific IgG and IgM Antibodies in Human Whole Blood, Serum, or Plasma. During the test, the sample is dropped into the sample well of the reagent, and the chromatography is performed under the capillary effect. The human monkeypox antibody (IgG and IgM) in the sample binds to the colloidal gold-labeled monkeypox antigen, diffuses to the test area, and is captured by coated monkeypox monoclonal antibody II (anti-human IgG and anti-human IgM), forming a complex to aggregate in the test area (test line IgG and test line IgM); the quality control area is coated with goat anti-mouse IgG antibody, which captures the colloidal gold-labeled antibody to form a complex and aggregate in the quality control area. The highly specific antigen-antibody reaction and colloidal gold immunochromatography technology are combined to qualitatively detect the content of IgG and IgM antibodies to monkeypox virus in serum, plasma or whole blood. Test principle: the combination of the analyte with the capture antibody on the membrane and the colloidal gold labeled antibody produces a color change, and the color intensity change has a correlation with the concentration of the analyte.

RT-PCR

2. What is Reverse Transcription Polymerase Chain Reaction (RT-PCR)?

Reverse transcription-polymerase chain reaction is a molecular technique commonly called RT-PCR. PCR stands for polymerase chain reaction, a laboratory method used to amplify specific regions of DNA for diagnosis and analysis in medical research. Reverse transcriptase PCR, instead of DNA, uses mRNA as the starting template.

Real-time RT-PCR is a modified version of PCR in which the enzyme called reverse transcriptase is used. This enzyme facilitates the conversion of an RNA sequence into its complementary DNA sequence. RT-PCR works on this principle of reverse transcription. It is a real-time technique that combines reverse transcription of RNA into DNA and the amplification of target DNA. It’s a qualitative means of detecting a particular RNA sequence with a high degree of accuracy and sensitivity.

The Monkeypox virus nucleic acid Test Kit is performed on the applicable instrument to detect DNA extracted from lesion exudate specimens. It utilizes the monkeypox virus conserved sequence of F3L gene as multiplex real time PCR amplification target regions.

The fluorescent quantitative PCR instrument can automatically draw a real-time amplification curve based on the detected fluorescent signal, so as to realize the qualitative detection of the monkeypox virus at the nucleic acid level. The multiplex real time PCR detection system includes a pair of primers and probes for internal control (RNase P). The positive control and negative control included in the kit can be used as an external control in every test run. The results of internal and external control can be used to monitor correct specimen collection, handling and real time PCR process.

ELISA

3. What is Reverse enzyme linked immunosorbent assay (ELISA)?

ELISA is an antigen antibody reaction. It is a plate based assay technique which is used for detecting and quantifying substances such as peptides, proteins, antibodies and hormones. An enzyme conjugated with an antibody reacts with colorless substrate to generate a colored product.

ELISAs are typically performed in 96-well polystyrene plates. The serum is incubated in a well, and each well contains a different serum. A positive control serum and a negative control serum would be included among the 96 samples being tested. Antibodies or antigens present in serum are captured by corresponding antigen or antibody coated on to the solid surface. After some time, the plate is washed to remove serum and unbound antibodies or antigens with a series of wash buffer. To detect the bound antibodies or antigens, a secondary antibodies that are attached to an enzyme such as peroxidase or alkaline phosphatase are added to each well. After an incubation period, the unbound secondary antibodies are washed off. When a suitable substrate is added, the enzyme reacts with it to produce a color. This color produced is measurable as a function or quantity of antigens or antibodies present in the given sample. The intensity of color/ optical density is measured at 450nm. The intensity of the color gives an indication of the amount of antigen or antibody

Monkeypox Related Products

Total antioxidant status(TAS) and Total oxidant status(TOS) are two indicators to determine all antioxidants or oxidants in samples, which can more comprehensively reflect the changes of oxidant and antioxidant capacity of samples；TAS and TOS are widely used in clinical studies, animal or cell culture testing, and food or beverage testing.

Elabscience has developed total oxidant status and total antioxidant status assay kits with simple operation, time saving and ability of easily measuring all non-enzymatic antioxidants and oxidant molecules.

TAS and TOS Assay Kits Application

Why Choose Elabscience^® TOS and TAS Assay Kits

Elabscience^® TOS and TAS Assay Kits

TOS and TAS Detection Principles

Other Oxidative Stress Related Biomarker and Assay Kits

Oxidative stress is associated with the development of more than 100 diseases. It is evaluated by measuring the changes in the content of antioxidants or oxidants (oxidation products) in the system.

Oxidation product

Oxidants

Nonenzymatic antioxidants

enzymatic antioxidants

What is QuicKey Pro^™ ELISA ?

Shorter experiment time

Easier experimental process

Superior product performance

More convenient storage

VS

VS

Procedure of the QuicKey Pro^™ ELISA Kit

QuicKey Pro^™ Sandwich method

QuicKey Pro^™ Competitive method

QuicKey Pro^™ ELISA Product Information

Polyclonal antibody is a group of immunoglobulin secreted by plasma cells in the organism, which occurred during the immune reaction when the body is stimulated by heterologous antigens (macromolecular antigen, hapten conjugates). Polyclonal antibodies have been widely applied in science research and diagnosis due to the various advantages, such as recognizing multiple antigen epitopes, causing precipitation reaction, short preparation time and low cost.

The most important quality for polyclonal antibodies is high titer. Elabscience’s polyclonal antibodies are prepared from the antiserum of immunized animal by affinity purification, and this preparation method leads to the production of interested antibodies with high-titer and high-affinity which against the target antigen. We have collected a wide selection of high-quality polyclonal antibodies specific for various species and applications, and all of them are guaranteed to do great favor for your research.

A monoclonal antibody specifically recognizes a single epitope within an antigen, thus having following advantages compared with polyclonal antibodies, low cross-reactivity, high specificity, excellent lot-to-lot stability.

Elabscience^® offers Mouse Monoclonal antibody secreted by hybridoma cell which is fused with unique cell clones and malignant myeloma cells. They have the characteristics of high potency and stable quality. The Mouse Monoclonal antibody are with high potency and stable quality.

Elabscience^® also provides custom monoclonal antibody service, designing and producing high quality antibodies according to your project requirements, to benefit your research at the greatest extent.

Secondary antibodies can bind to primary antibody namely ‘anti-antibody’. Secondary antibody combines enzyme or fluorescent, and indirectly detects the target protein by binding with the primary antibody, thus amplifying the signal and improving the sensitivity of detection.

Elabscience^® offers various high-quality conjugated/unconjugated secondary antibodies which can be applied in fluorescence detection of the primary antibody, and they are suitable for conventional applications such as IF, IHC, WB and Flow cytometry, etc.

The history of human society is a history that human beings constantly fight against viruses and overcome various viruses through knowledge innovation and technological progress.

Malaria, black death, Spanish flu, Ebola virus, SARS in 2003 and theCOV-2019 have taken many people’s live. In particular, COV-2019 is stilloutbreak in the global world and many families lost their love.

In the process of human civilization development, we are suffering from infectious diseases caused by viruses. Every time when a new virus appears, scientists need to spend a lot of time looking for ways to deal with the virus.

ELabscience^® has been committed to providing effective solutions for researchers to supporting the study of infectious diseases. Which includes Cov-2019，SRAS-Cov，MERS-Cov and other virus like HIP virus，HPV virus，and so on.

These antigens and antibodies are specifically developed to help science researcher to learn more about virus and its infection mechanism.

Strict quality control
KO correlation verification was carried out to ensure the success of target knockout at the gene level and protein level.

Present stock
A manufacturer that launch KO validated antibodies.

Reliable product
The KO products can be directly tested to avoid cumbersome antibody data judgment and multiple validation of quality again.

HMGB1 Polyclonal Antibody
Cat.No.:E-AB-60726
Reactivity:Human,Mouse,Rat
Application:WB,IHC,IF

LC3B Polyclonal Antibody
Cat.No.:E-AB-61580
Reactivity:Human,Mouse,Rat
Application:WB,IHC,IF

DNMT1 Polyclonal Antibody
Cat.No.:E-AB-60994
Reactivity:Human
Application:WB,IF

FANCD2 Polyclonal Antibody
Cat.No.:E-AB-67288
Reactivity:Human
Application:WB

Acetylation is related with protein activity, therefore the activity of target protein can be reflected through the specific identification of acetylated target protein by acetylated antibodies, and to study the function and regulation of protein.

Phosphorylation antibodies can identify the phosphorylation or non-phosphorylation of protein, and can be applied in qualitative and quantitative analysis of phosphorylated protein under the condition of avoid radiation pollution. Elabscience’s specific phosphorylated antibodies can be widely used in applications such as Western blot, Immunohischemistry, ELISA, etc.

Methylation is an important modification of proteins and nucleic acids, which can regulate the expression and silence of gene and is closely associated with cancer, aging, Alzheimer’s disease and other diseases, is one of the most important research content of epigenetics. The most common methylation modification includes DNA methylation and histone methylation.

Learn about the diverse collection of Acetyl/Phospho/Methyl Antibody in Elabscience and know how they can meet the needs of your researches.

Elabscience^® Ready-to-Use Antibodies for IHC are high-quality monoclonal antibody, which has been repeatedly verified by the immunohistochemistry experiment of positive samples. These antibodies are mainly used for the identification, characterization and localization of intracellular or extracellular proteins. They are tested strictly to ensure the Ready-to-Use effect of the product and are cost-effective. This product is suitable for all kinds of immunohistochemistry kits produced by various manufacturers. It can be operated on different immunohistochemistry experimental instruments to avoid false negative caused by improper dilution of pre experimental antibody and save your precious samples and experimental time.

Elabscience^® is committed to improve the quality and variety of antibodies to speed up your research process. Those products are 100% guaranteed , please feel free to consult and purchase.

Ki67, Human Tonsil

β-Catenin, Human Colon Cancer

E-Cadherin, Human Liver Cancer

Vimentin, Human Liver Cancer

Recombinant rabbit mAb combines the advantages of the rabbit immune system and recombinant protein technology including improved specificity, high affinity, stable product quality and high batch-to-batch consistency. It can be applied for long-term and large-scale research and has widely used in scientific research and diagnosis.

Elabscience^® provides a variety of recombinant rabbit mAbs and can be used in various biochemical experiments such as WB, IHC-P, ICC/IF to meet your needs.

Featured Elabscience^® Recombinant Rabbit mAb Products

Recombinant Bcl2 Monoclonal Antibody

Immunohistochemistry of Bcl-2 in paraffin-embedded Human breast cancer tissue using Bcl-2 Rabbit mAb(E-AB-81425) at dilution 1:100

Recombinant CDK2 Monoclonal Antibody

Immunohistochemistry of Cdk2 in paraffin-embedded Human tonsil using Cdk2 Rabbit mAb(E-AB-81426) at dilution 1:100

Recombinant Smad3 Monoclonal Antibody

Immunohistochemistry of Smad3 in paraffin-embedded Human breast cancer tissue using Smad3 Rabbit mAb(E-AB-81437) at dilution 1:100

Recombinant FOXO4 Monoclonal Antibody

Western blot detection of FOXO4 in K562,C6,3T3 cell lysates using FOXO4 Rabbit mAb(E-AB-81558)at dilution 1:1000

Recombinant AKT Monoclonal Antibody

Western blot detection of AKT1/2/3 in Jurkat,Rat Brain,C6,CHO-K1,Hela cell lysates using AKT Rabbit mAb(E-AB-81455)at dilution 1:1000

Recombinant YTHDC2 Monoclonal Antibody

Western blot detection of YTHDC2 in Hela,A549,HL-60,U251,U87-MG cell lysates using YTHDC2 Rabbit mAb(E-AB-81516) at dilution 1:1000

SARS-CoV-2 is a virus that can cause severe acute respiratory failure and spread on a large scale in the population. Its existence and transmission pose a serious threat to human life and physical health, and it has caused the most serious public health incident in the world in recent years.

SARS-CoV-2 has several structural proteins including spike (S), envelope (E), membrane (M) and nucleocapsid (N). The spike protein (S) contains a receptor-binding domain (RBD), which is responsible for recognizing the cell surface receptor, angiotensin converting enzyme-2 (ACE2). It is found that the RBD of the SARS-CoV-2 S protein strongly interacts with the human ACE2 receptor leading to endocytosis into the host cells of the deep lung and viral replication.

To effectively and quickly screen out SARS-CoV-2 asymptomatic, severely ill patients and recovered patients among large-scale populations, the application of molecular biology and immunology technology is particularly important. In order to help the SARS-CoV-2 research, Elabscience has developed 9 types of SARS-CoV-2 related antigen and antibody ELISA kits, which can meet customer research needs.

Focus on Developing and Manufacturing ELISA Assay Kits Set for Your Academic Research

Elabscience offers thousands of enzyme-linked immunosorbent assay (ELISA) kits for researchers, The species include human, mouse, rat, rabbit, monkey, porcine, etc. As a professional ELISA kits manufacturer and supplier, our ELISA test kits have been strictly quality-controlled to ensure the accuracy of results. Elabscience is committed to providing high quality ELISA kits with a very economical price. We always take the “customer oriented”, provide the best and professional services to customers. You can contact our distributors all around the world for purchase, or you can also buy our ELISA kits online directly.

QuicKey ELISA^®

QuicKey ELISA^® kit developed by Elabscience is an improvement of the traditional sandwich ELISA kit. It simplifies the operation process, saves at least 1h and requires less sample volume for assays, greatly improves the detection effciency.

Sandwich ELISA

Sandwich ELISA (or sandwich immunoassay) is the most commonly used elisa method. This format requires two antibodies/antigens specific for different epitopes of the antigen/antibodies. These two antibodies/antigens are normally referred to as matched antibody/antigen pairs. One of the antibodies/antigen is coated on the surface of the micro plate and used as the capture antibody/antigen to immobilize the tested analyte. The other antibody/antigen is conjugated with biotin or other markers to facilitate the detection of the analyte.

Competitive ELISA

Generally, Competitive ELISA (or competitive immunoassay) is applied for small molecular detection. The sample antigen competes with the pre-coated antigen for binding to a specific amount of labeled antibody. This assay only requires a single antibody conjugated to biotin commonly to facilitate the detection of the analyte.

Nature IF: 42.778

DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25

Nature Medicine IF: 36.13

Implant-Derived Magnesium Induces Local Neuronal Production Of CGRP To Improve Bone-Fracture Healing In Rats

Cell IF: 38.637

Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output

Advanced materials IF:27.398

Dual‐Locking Nanoparticles Disrupt the PD‐1/PD‐L1 Pathway for Efficient Cancer Immunotherapy

Food safety is an increasingly concerned problem all around the world. In order to ensure the safety of food and feed, more than 100 countries have formulated food and feed related regulations. Elabscience^® is a global supplier of food and feed safety solutions. We provide a wide range of innovative testing solutions and services for qualitative and quantitative detection of mycotoxins, residues and contaminants in food and feed.

Introduction

The lateral flow assay kit is rapid detection cards using immunogold gold technology. The test method is based on antigen-antibody reaction. The liquid sample droplets are added to the sample hole. If the target substance exists, a color zone will be seen on the test line (T) in 8 minutes. This visual evaluation enables qualitative analysis. This rapid detection card of immunogold gold technology is easy to operate and low cost.

Advantages of Lateral Flow

• Rapid judgment of results
• No laboratory equipment required
• Suitable for field analysis
• High stability
• Free of harmful substances
• No training costs

Introduction

Enzyme-linked immunosorbent assay established a quantitative ELISA method based on the selectivity and specificity of antibody and antigen recognition. The main principle of ELISA: one of these immune components is fixed in the holes of the solid phase (enzyme plate), and the detected target in the sample interacts with the antibody-antigen system. This interaction can be observed by enzymes in enzyme markers and indicate whether antibody-antigen binding occurs. The added substrate is transformed by coupling enzyme, resulting in color change, which can be measured by Microplate Reader.

Advantages of ELISA

• High sensitivity: ppb(ng/mL) level
• Quantitative analysis and no radioisotope pollution
• Low batch-to-batch variation
• Good specificity
• Long valid period
• Suitable for multiple samples

Elabscience^® metabolism assay kits has a complete set of indicators and a wide range of applications, including Enzymes, Oxidative stress, Liver biomarkers, Kidney biomarkers, Amino acids & proteins, Glycolysis & Carbohydrates, Inorganic ions, Lipids metabolism, Plant stress resistance, Tricarboxylic Acid（TCA）Cycle, etc. Our assay kits suitable for a variety of sample types and the commonly used detection instruments which have spectrophotometer, microplate reader, fluorescence microplate reader, flow cytometer. Elabscience^® metabolism assay kits has experienced 5 years of painstaking scientific research to create exclusive quality, and sold to more than 100 countries around the world. You can contact our distributors all around the world for purchase, or you can also buy our assay kits online directly.

• Serum
• Urine
• Saliva

• Muscle
• Liver Tissue
• Pathological Tissue

• Juice
• Honey
• Milk

• Drug
• Botany
• Cosmetics

Immunolabelling technology is an immunobiochemistry analysis method to identify and determinate the trace and ultra-trace bioactive substances. The principle of immunolabeling is to covalently combine the marker (such as fluorescein, radioactive isotope, enzyme, biotin, etc.) with another compound, and then a multicomponent complex will form due to the specific reaction between the marked compound and tested compound. After the separation of unbound marked compound, you can observe and detect the experiment result with kinds of precise detection instruments.

The immunolabeling technology can be applied to take qualitative, quantitative or semi-quantitative analysis of samples, and it can also be used to study the location in cells or tissues. Elabscience^® offers Fluorescence Labeling Kits and Biotin Labeling Kits with high-quality for researchers. Browse all Labeling Kits in below table.

Primary cells refer to individual cells obtained from tissues or organs of the body through trypsin, collagenase, neutral protease or other methods and cultured in vitro to simulate the environment of the body. Generally, the cultured cells within the first to tenth generation are collectively referred to as primary cells. In the absence of genetic and chemical modification, primary cells retain many important physiological characteristics of their original tissue system and can well mimic in vivo conditions. Therefore, it provides an ideal cell model for research fields such as cell biology, physiology, developmental biology, oncology, immunology, and even the research on the pathogenic mechanism of diseases and drug treatment.

Elabscience is committed to creating a professional primary cell research and development and service platform, providing hundreds of types of fat, skin, lung, liver, kidney, pancreas, intestines from rats, mice, rabbits, chickens, etc. Cells in multiple tissue sites.

A cell line is a cell culture developed from a single cell and therefore consisting of cells with a uniform genetic makeup. Cell line allow us to dissect the internal workings of tissues in a controlled environment without the ethical implications of working with whole organisms. Elabscience is committed to providing common experimental cell lines to laboratories around the world, such as tumor cells, normal cells, and resistant drug cell lines. All human cell lines in the bank provides STR identification reports, and mouse cell lines provide specie identify reports. The cell line is shipped from the stock and professional technical guideline is provided free of charge throughout the entire process to ensure that you have no worries after sales.

●Reliable source

●Store in liquid nitroge

●Eliminate cross contamination

●Stringent quality control

Apoptosis is a complex and active death, involving the activation, expression and regulation of a series of genes.

When apoptosis occurs, a series of apoptotic processes are programmed, such as phosphatidylserines valgus outside of the cell membrane, mitochondrial membrane ΔΨm changes, caspase activation, DNA fragmentation and so on.

Abnormal apoptotic mechanism can cause cells to lose control, which is closely related to the occurrence and development of many diseases such as tumors. Accurate detection of apoptotic cells is essential in the apoptosis study.

Elabscience^® has successfully developed multiple apoptosis detection products based on the most commonly used principles.

DNA Fragmentation

When cells undergo apoptosis, a large number of viscous 3′-OH are produced by breakage of chromosomal DNA. Under the action of deoxyribonucleotide terminal transferase (TdT), the deoxyribonucleotides and derivatives of fluorescein, peroxidase, alkaline phosphatase or biotin can be labeled to the 3′-terminal of DNA, so that apoptotic cells can be detected.

Caspase Activacion

Caspase family plays a very important role in the process of cell apoptosis. Caspase-3 is a key executive molecule, which plays a role in many pathways of apoptotic signal transduction. Caspase exists in the cytoplasm in the form of zymogen and in the early stage of apoptosis, the caspase is activated which eventually leads to apoptosis.

Phosphatidylserine Extemalization

It occurs at early apoptosis and runs through the whole cell apoptosis. Annexin V can bind to valgus phosphatidylserine under the action of Ca2+. Annexin V with DNA dyes such as PI and 7-AAD can distinguish living cells, early apoptotic cells, late apoptotic cells and dead cells.

Decrease of Mitochondrial Transmembrane Potential

The decrease of mitochondrial membrane potential is considered to be the earliest event in process of cell apoptosis. JC-1 dye exhibits different fluorescence emission due to different mitochondrial membrane potential. Cell apoptosis can be detected by detecting the change of cell membrane potential by JC-1 emission light.

Introduction

Elabscience^® Caspase 3 Activity Assay Kit can detect the activity of Caspase 3 in cells or tissue lysis.

Detection principle

Caspase (Cysteine-requiring Aspartate Protease) is a protease family that plays an important role in the process of apoptosis. Caspase 3 (also known as CPP32, Yama, or apopain) is a member of the CED-3 subfamily of Caspases and is one of the critical enzymes of apoptosis. Caspase 3 is the most studied Caspase in mammalian cells. Caspase 3 can be used to cut proCaspase2, 6, 7, 9, and cut specifically many substrates of Caspase directly, including PPAR (poly ADP-ribose), the inhibitor of Caspase-activated deoxyribonuclease (ICAD), gelsolin and fodrin. Protein shearing mediated by Caspase 3 are important parts of the molecular mechanism of apoptosis. In addition, Caspase 3 also plays a key role in the process of nuclear apoptosis including chromatin condensation and DNA fragmentation as well as cell blebbing. Caspase 3 exists in the form of prozyme in the normal state and has no activity. However, during the apoptosis stage, activated Caspase 3 consists by two large subunits and two small subunits, which cleavages the corresponding substrate of endochylema or cytoplasmic nuclear and eventually leads to apoptosis.

This kit is used to conjugate Caspase 3 sequence-specific peptides acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) to yellow group p-nitroaniline (pNA). When the substrate is cut by Caspase 3, the yellow group pNA is dissociated. pNA has an absorption peak at 405 nm. Measure the OD value at 405 nm and then

Caspase 3 activity can be calculated accordingly.

Components

Reagents not included

PBS, Protein Quantitative Kit (Bradford, optional).

Storage

Store at -20°C. Ac-DEVD-pNA[E-CK-A311C] should be stored in dark. Avoid freeze / thaw cycles.

Note

1. For maximal assay performance, this reagent should be used within 12 months. Avoid freeze / thaw cycles.
2. There is reducing agent (DTT) in the Lysis Buffer,It is recommended toElabscience^® Total Protein (TP) Colorimetric Assay Kit (Coomassie Brilliant Blue Method)(It is recommended to use Elabscience^® E-BC-K168-S) to measure the protein concentration instead of BCA method.
3. It has been reported that the activity of Caspase 3 can’t be detected in few types of apoptosis, which may be due to the existence of a mechanism independent of the activation of Caspase 3, and other signaling pathways in the mechanism of apoptosis need to be considered. In this case, there is no significant change in the activity of Caspase 3 by using this kit.
4. Lysis Buffer in this kit can be used in other Caspase activity assay kits byElabscience^®. And the protein sample can test other Caspase activity.
5. pNA (4-nitroaniline) is toxic. Please be careful when operating, and pay attention to effective protection to avoid direct contact with human body or inhalation. pNA solidifies at lower temperatures and sticks to the bottom, wall or cap of centrifugal tube. It can be incubated in water bath at 20~25°Cfor a short time until it is completely melted.
6. When the activity of Caspase 3 in the sample is very low. Confirm whether the phenomenon of apoptosis is obvious or not firstly. If the apoptosis is obvious and we confirm that Caspase can be activated, please adjust the time of apoptosis and find a time point which Caspase 3 activation is stronger. And then repeat the test.
7. This kit is for research use only.For your safety and health, please wear lab clothes and gloves. Instructions should be followed strictly, changes of operation may result in unreliable results.

Cell proliferation is an important life characteristic of organisms which is used to replenish senile or dead cells in the body. MTT Assay Kit (Cell Proliferation) has been widely used to detect cell proliferation and cytotoxicity. In recent years, the CCK-8 method has gradually replaced the traditional MTT method because of its high sensitivity and non-radioactivity.

Elabscience^® Enhanced CCK-8 kit is more sensitive and with wider linear for detecting the number of living cells in cell viability, proliferation or cytotoxicity test.

• High sensitivity
• Wide linear and perfect reproducibility
• Low toxicity, simple operation
• Multiple applications, drug screening, tumor drug sensitivity etc.

Hela cells: 100 μL/well
CCK-8 solution: 10 μL/well
Incubation: 1 h
Detection: Absorbance value (450 nm)

Since the outbreak of the SARS-CoV-2, the spread of COVID-19 and its impact on public health have continued to ferment. Researchers around the world are working to deepen their understanding of coronaviruses with a view to quickly determine future treatment options and possible vaccine targets. As a global supplier of life sciences, during this special period, Elabscience^® is committed to providing necessary products and services for researchers, developers and manufacturers to help them cope with 2019-nCoV.

More Abundant Products

Elabscience provides most targets of SARS-CoV-2

• Structural proteins
• Non-structural proteins
• Host-related proteins of SARS-CoV-2
• Different functional regions of the same target
• Different expression systems
  (bacteria, yeast and mammals)
• Different tags (His, Fc, mFc, rFc)

Excellent Product Performance

Elabscience owns a professional and efficient protein research and development team in immunology testing field.

• The SARS-CoV-2 proteins showed:
• Stable quality
• High purity
• Low endotoxin

Some proteins have been verified by HPLC and activity detection, which can meet the high requirements of scientific researchers.

Perfect Pre-sale and After-sale Service

Elabscience provides fast and professional technical services to answer every question that researchers care about.

We have a mature delivery team and rich experience to ensure that customers can get our products in the shortest time,so that you can buy it and use it in a more convenient way.

The SARS-CoV-2 is causing great damage to human health all over the world. SARS-CoV-2 is a RNA virus, which is more prone to mutation than DNA virus. There are two aspects of SARS-CoV-2 mutants that are thought-provoking: First one is spike protein (s), especially the key receptor binding domain (RBD). Mutation or mutation accumulation may enhance the binding ability of SARS-CoV-2 spike protein to receptor ACE2, and increase the transmission or pathogenicity of the virus. The other one that the mutation may change the key epitope of antigen, reduce the affinity of screened neutralizing antibody, cause immune escape, and reduce the protective effect of vaccine and neutralizing antibody.

Elabscience^® has developed nearly 140 SARS-CoV-2 mutant proteins, which can be used to study the potency of antibodies and vaccines.

Study the characteristics

The binding ability of the mutant protein N501Y(U.K. Variant) to ACE2 increased nearly 9 times, while the binding ability of the mutant proteins K417N,E484K and N501Y(South Africa Variant ) to ACE2 increased nearly 3 times.

Detect the neutralizing antibody reaction

The latest research shows that there is antibody escape in SARS-CoV-2 mutants, and the efficacy of antibody drugs approved or being developed against SARS-CoV-2 will be uncertain.

Verify the SARS-CoV-2 antigen detection kit

The antigen detection kit mainly uses N protein as the detection marker. In the process of mutation, the N protein of SARS-CoV-2 also showed some high frequency mutations.

Evaluate the effect of SARS-CoV-2 vaccine

The impact of the SARS-CoV-2 mutant to the vaccines is a widely concerned problem. Some enterprises have begun to lay out the development plan for the mutant vaccine.

Recombinant protein is a manipulated form of protein produced through recombinant DNA technology. By inserting the DNA encoding the protein into bacterial or mammalian cells, we can get quantities of target proteins after amplifying expression and purification. Elabscience^® offers a diverse selection of more than 4000 proteins for your research, including cytokines, growth factors, immune checkpoint proteins, CD antigen, FC Receptor, enzymes, hormone, etc.

Elabscience^® offers over 1000 different active proteins ready for experimental usage. There are a wide range of biological assay methods to measure the bioacitivity of a recombinant protein, including proliferation assay, cytokine induction assay, chemotaxis assay, cell proliferation assay, enzyme assay, funtional ELISA , etc. The recombinant proteins that validated bioactivity are available for cell culture, drug target research, candidate drugs, structural study, protein-protein interaction study and many other research fields.

Detection of Apoptotic Camptothecin-treated Jurkat by Annexin V Staining.

Jurkat were left untrated (left) or treated with 1 μM Camptothecin treated for 4 h (right) and then stained using Annexin V-APC. The combination of Annexin V(PKSH033460), APC labeled allows for the distinction between apoptotic cells (Annexin V-APC positive) and viable cells (unstained).

Recombinant human IL-6(PKSH033611) was measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 20-100pg/ml.

Elabscience^® Cytokines are small secreted proteins released by immune cells that serve to regulate the immune system, which involve in autocrine, paracrine and endocrine signaling, also regulate the maturation, growth and responsiveness of various cell types. Elabscience^® provides a comprehensive catalog of recombinant cytokines with excellent lot-to-lot consistency, superior activity and significantly low endotoxin levels, including Chemokines, Interferons(IFNs), Interleukins, and Tumour Necrosis Factors(TNFs), Cylony Stimulating Factors(CSFs), Growth Factors and Other Cytokine-Related Proteins.

Immune checkpoints are regulators of the immune activation. They consist of stimulatory and inhibitory pathways that maintain self-tolerance and assist with immune response. Stimulatory Immune checkpoints augment immunological responses against pathogens. On the contrary, inhibitory Immune checkpoints negatively regulate T-cell activation. In cancer, immune checkpoint mechanisms are often activated to suppress the nascent anti-tumor immune response, and PD-1, PD-L1, CTLA-4, CD28, OX40 are hot targets for cancer immunotherapy.

Chromatography is a most frequently used laboratory technique for separation and purification of biomolecules mixture. The mixture is dissolved in the mobile phase (a kind of fluid), which carries it through the stationary phase (a structure holding another material). The separation is based on differential partitioning between the mobile and stationary phases.

According to the different separation mechanism and principle, chromatography techniques are classified into different types for separation and purification process of the various target biomolecules. There are 4 types of commonly used chromatography techniques: Affinity chromatography, Ion exchange chromatography (IEC), Gel filtration (also known as Size-exclusion chromatography (SEC) and molecular sieve chromatography), and Hydrophobic interaction chromatography (HIC).

As a professional biotechnology company devoted to provide the best reagents and consumables for scientists, Elabscience^® is committed to expand our product line and more high-quality products and services for scientific research. We have collected the most popular and acclaimed chromatographic media in one page. View and discover the enjoyable products!

Affinity chromatography

Affinity chromatography is a powerful chromatography technique of separating and purifying proteins based on the highly specific interaction between antigen and antibody, enzyme and substrate, or receptor and ligand. Such interactions including hydrogen bonding, ionic interaction, disulfide bridges, hydrophobic interaction, etc. Affinity chromatography are widely recognized for their high selectivity, high resolution and high capacity.

Ion exchange chromatography

Ion exchange chromatography (IEX) is a practical chromatography process that separates ions and polar molecules based on their respective charged groups. It works on almost any kind of charged molecule—including large proteins, small nucleotides, and amino acids.

Gel filtration chromatography

Gel filtration chromatography, also known as size exclusion chromatography (SEC), is a chromatographic method that separates molecules by their size or molecular weight. SEC can be used for wide ranges of separation and purification of components with large molecular weight differences and low resolution requirement, such as proteins, polysaccharides and other macromolecules.

Hydrophobic interaction chromatography

HIC is a useful method for purification and separation of biomolecules based on their surface hydrophobicity. HIC can be applied in the separation and purification of hydrophobic proteins such as aromatic and aliphatic compounds. In HIC, the matrix material is lightly substituted with hydrophobic groups, such as methyl, ethyl, propyl, octyl, or phenyl groups.

Introduction

Proteinase K is a kind of high activity Proteinase of subtilisin, which is used to degrade proteins in biological samples. It can be used to digest various proteins, and it can be used in a variety of molecular biology, cell biology and other related experiments, such as genomic DNA extraction, enzyme digestion and removal, cell permeability and so on.

Enzyme activity, > 30 U/mg. At 37°C, the amount of Proteinase K that can produce amino acids or polypeptides equivalent to 1 micromol of tyrosine Folin positive in one minute with hemoglobin as the substrate is defined as a unit of Proteinase K activity.

The effective pH range of Proteinase K is pH4.0~12.5, and the optimal pH range is pH7.5~8.0.

The optimum reaction temperature of Proteinase K is 65°C, but at 65°C or higher, Proteinase K can also degrade rapidly. It is suggested that the optimum reaction temperature is 50~55°C.