Aminoallyl-dUTP-XX-Cy3
Catalogue Number: NU-803-XX-CY3-L-JEN
| Manufacturer: | Jena Bioscience |
| Shelf Life: | 12 months |
| Ph: | 7.5 +/-0 |
| Physical state: | Solution |
| Type: | Labelled Nucleotides |
| Shipping Condition: | Blue Ice |
| Unit(s): | 5 x 10 ul |
Description
Description: Aminoallyl-dUTP-XX-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Higher incorporation and thus labeling rates can be achieved compared to Aminoally-dUTP-Cy3 (NU-803-Cy3). Recommended Aminoallyl-dUTP-XX-Cy3/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-Cy3/ 50% dTTP Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
Additional Text
Concentration
1.0-1.1 mM
Application Notes
Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I in-house data
Short Description
5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with Cy3, Triethylammonium salt
Smile String
C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCCCN1c2ccc(S(=O)(=O)O)cc2C(C)(C)/C/1=CC=CC1=[N+](c2ccc(cc2C1(C)C)S(=O)(=O)[O-])CC
Molecular Weight
1362.29 g/mol (free acid)
Molecular Formula
C55H78N7O23P3S2 (free acid)
NU-803-XX-CY3-L MSDS
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