H3pan (1B2) Monoclonal Antibody
Catalogue Number: BSM-53008M-BSS
BSM-53008M Image
BSM-53008M Image
BSM-53008M Image
BSM-53008M Image
Western blot was performed on whole cell extracts from HeLa cells (25 µg, lane 1) and recombinant histone H2A, H2B, H3 and H4 (1ug, lane 2, 3, 4 and 5, respectively) using the H3pan (1B2) Monoclonal Antibody (bsm-53008M). The antibody was diluted 1:1,000 in TBSTween containing 5% skimmed milk, followed by secondary antibody incubation and exposure.
BSM-53008M Image
Western blot was performed on whole cell extracts (30µg) from different cell types (lane 1: HeLa, lane 2: K562, lane 3: MCF7, lane 4: U2OS, lane 5: HepG2, lane 6: Jurkat, lane 7: NIH3T3, lane 8: E14Tg2a mouse ES cells) using H3pan Monoclonal Antibody (bsm-53008M) at 1:1,000 dilution in TBS-Tween containing 5% skimmed milk, followed by secondary antibody incubation and exposure.
BSM-53008M Image
HeLa cells stained with the H3pan Monoclonal Antibody (bsm-53008M) and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 1% BSA. The cells were labeled with the H3 antibody (middle) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The left panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
| Manufacturer: | Bioss Inc |
| Shelf Life: | 12 months |
| Preservative: | 0.05% Sodium azide |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | 1B2 |
| Isotype: | IgG3 |
| Immunogen: | Monoclonal antibody raised in mouse against histone H3, using a KLH-conjugated synthetic peptide containing an unmodified sequence from the C-terminus of the protein. |
| Application: | ICC, IF, WB |
Description
Description: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Additional Text
Uniprot ID
P68431
Purification
Protein A purified
Gene ID
8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968
Concentration
0.5 ug/ul
Antibody Clonality
Monoclonal
Modification
Unmodified
Gene Name
H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8
Storage Note
Store at -20°C for 12 months.
bsm-53008M Datasheet
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