Vazyme Biotech is devoted to the development and production of high quality enzyme- and antibody-based products for molecular biology applications. Products are used for basic research, clinical diagnosis, molecular diagnostics, high-throughput sequencing and life science research and other related fields. Competitively priced, Vazyme products deliver exceptional value without compromising performance.
Vazyme offers products for:
• PCR – Conventional PCR, High Fidelity PCR, High Yield PCR, Rapid PCR, Long Fragment PCR, Hot Start PCR, Multiplex PCR, Genotyping Kits, and Isothermal Amplification
• qPCR –SYBR-based and probe based, Low/High ROX, master mix and hot-start versions
• RT-PCR - Conventional RT-PCR, One-Step RT-PCR, Tissue and Cell RNA Storage and Total RNA Extraction kits
• DNA Cloning – Rapid, highly efficient One-Step Cloning kits
• Next Generation Sequencing (NGS) – Library construction for RNA-seq, DNA-seq and single cell libraries
• Cell- based assays – including Mycoplasma detection and transfection reagents
Examples of key products include:
Phanta® Max Super-Fidelity DNA Polymerase
is delivers robust PCR with extreme fidelity, with an error rate 53-fold lower than that of Taq polymerase and extension rate as fast as 1 sec / kb (depending on application). This enzyme possesses 5´→ 3´polymerase activity, 3´→5´exonuclease activity and generates blunt-ended products, and is also capable of amplifying long fragments such as 20kb genomic DNA and 40kb λDNA. The recommended applications include cloning, sequencing, genomic DNA amplification and high throughput PCR
AceQ qPCR SYBR® Green Master Mix
uses a special performance-enhanced hot-start Taq DNA polymerase with an optimized qPCR buffer system to perform SYBR Green I based quantitative PCR (qPCR). The mix, prepared at 2× reaction concentration, can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability
AceQ qPCR probe Master Mix
is a convenient 2x premixed reagent for probe-based qPCR which contains a hot-start AceTaq™ DNA polymerase
along with a specially optimized buffer which significantly reduces non-specific amplification and enhances amplification efficiency.
ClonExpress II One-Step Cloning Kit
uses a highly efficient homologous recombination technology to clone DNA products to very high efficiency in a simple rapid one-step procedure. Any amplified product(s) can be cloned into to any linearized vector, at any site. The kit uses Exnase II, a unique recombinant enhancer which can significantly improve the efficiency of recombination (100 cfu/ ng Vector) even using low efficient competent cells (107 cfu/μg). Digested products or PCR products can be used directly in the recombination reaction without purification, which greatly simplifies the procedure.
TruePrep DNA Library Prep Kit V2 for Illumina
® is an optimized kit for next-generation sequencing on the Illumina platform. This kit contains enzymes and buffers to convert as little as 1ng of input DNA into indexed libraries in less than 90 minutes. Unlike traditional library construction methods, TruePrep Kit takes advantage of a new-type transposase to convert the complex steps of DNA fragment, end repair and adaptor connection into a one-step enzymatic reaction, which significantly reduces the amount of the initial DNA needed and shortens library construction time. All reagents provided in this kit are strictly quality and functionally controlled to guarantee the highest levels of stability and repeatability of library construction.
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