Figure 1. Excitation (left) and emission (right) spectra of DyLight fluorescent dyes conjugated to affinity-purified secondary antibodies. This figure illustrates the relative shape and position of each fluorophore in the peak region of its excitation and emission following conjugation to antibodies. Quantitative comparisons should not be made since peak heights have been normalized. All spectra were obtained with a M-Series spectrofluorometer system from Photon Technology International, Inc.