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DyLight 488
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Figure 4. Fluorescence intensity was measured over time and plotted as a percent of the initial intensity for HEp-2 cells labeled with ANA (BIO-RAD) and goat anti-human IgG (H+L) conjugated with various fluorophores. The cells were mounted in PBS containing 90% glycerol without anti-fading agents. |
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Figure 5. Fluorescence intensity was measured over time and plotted as a percent of the initial intensity for HEp-2 cells labeled with ANA (BIO-RAD) and goat anti-human IgG (H+L) conjugated with DyLight 488. The cells were mounted in various mounting media. |
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