Figure 6. HEp-2 cells were stained with ANA antibody (BIO-RAD) and with the indicated fluorophores conjugated to comparable secondary antibodies. For fluorescence microscopy cells were mounted in ProLong Gold (Invitrogen) (upper images) or Vectashield (Vector Labs) (lower images). Images were taken at 100X with a Nikon D-70 DSLR camera connected to a Nikon LABOPHOT epi-fluorescence microscope. Fields with the brightest cells for each fluorophore were selected for imaging after 1 second exposure to light.