Figure 7. Fluorescence intensity was measured over time and plotted as a percent of the initial intensity for HEp-2 cells labeled with ANA (BIO-RAD) and goat anti-human IgG (H+L) conjugated with various fluoro-phores. The cells were mounted in PBS containing 90% glycerol without anti-fading agents.

Figure 8. Fluorescence intensity was measured over time and plotted as a percent of the initial intensity for HEp-2 cells labeled with ANA (BIO-RAD) and goat anti-human IgG (H+L) conjugated with various fluoro-phores. The cells were mounted in ProLong Gold (Invitrogen) anti-fading medium. Similar results were obtained when the cells were mounted in Vectashield (Vector Labs) or PBS-glycerol containing n-propyl gallate (Fluka).