HumanZyme is a leading provider of recombinant human protein reagents to research, diagnostic and pharmaceutical industries. Currently, HumanZyme focuses on human cytokines and kinases from human cells, which are more authentic than the proteins expressed from any other species. HumanZyme are committed to provide authentic human proteins with superior quality:
• Fully validated gene sequences
• Extensive purity
• Unrivalled biochemical activity
Further information
Technical White Papers
Product Range
Recombinant Human Cytokines from Human Cells
Cytokines are a group of proteins and polypeptides that organisms use as signalling molecules. Most cytokines are glycoproteins less than 30 kDa in size and bind to specific, high-affinity cell surface receptors. Due to their central role in the immune system, cytokines are involved in a variety of immunological, inflammatory and infectious diseases and widely used in research, diagnostics and therapeutics. Currently, these proteins are predominantly produced in non-human cells (e.g. E coli ) and therefore lack authenticity due to the absence of relevant glycosylation. In addition, a number of important cytokines are not commercially available due to inadequate proteolytic processing or other post-translational modification that occur in the non-human cells.
HumanZyme has developed an efficient human cell-based technology, Huma Xpress, for medium and large scale production of human cytokines. Currently, more than 60 tag-free cytokines have been successfully produced, including difficult-to-express proteins of the TGF b superfamily. Using GM-CSF, IL4 and VEGF165 as examples, we demonstrate our high-authenticity cytokines from human cells can be used as highly preferred reagents for inflammation, cancer, stem cell research, and antibody development.
Ultra-low Endotoxin Recombinant Human from Non-human Cells
Endotoxins are frequent contaminants in the cytokines prepared from bacterial and other expression systems. Even low levels of endotoxins can be toxic to cells or organisms and must be removed. The industry standard reported value for commercially supplied cytokines is <1.0 EU/ m g. HumanZyme now offers researchers an expanding range of human cytokines exhibiting ultra-low levels of endotoxin contamination. These cytokines have been prepared to yield 10x to 1000x less endotoxin than the levels reported in standard commercial preparations. Ultra-low endotoxin cytokines are cost effective even for large scale studies.
Recombinant Human and non-human Cytokines from Non-human Cells
Currently, HumanZyme provide a range of recombinant cytokines produced in non-human cells with superior quality.
Recombinant Human Protein Kinases
Protein kinases act on and modify the activity of specific proteins via phosphorylation. These are used extensively to transmit signals and control complex processes in cells. Up to 518 different kinases have been identified in human genome. Due to the critical roles in intracellular communication, dysregulation of protein kinases has been implicated in as many as 400 human diseases including cancer, diabetes, heart diseases, neurological disorders and rheumatoid arthritis. Hence, individual protein kinases are important for drug target validation, protein crystal structure analysis, and drug design and screening. Currently, kinases are predominantly produced in non-human cells (e.g. E coli or insect cells) many of which involve protein truncation and/or in vitro activation, due to the limitations of the expression system.
HumanZyme has developed an efficient human cell-based technology, Huma Xpress, for the rapid production of active recombinant human protein kinases which are full length, in vivo activated, and highly authentic. Currently, we have successfully produced more than 100 active human protein kinases in an engineered human cell line. Using p38 a and MK2 as examples, we demonstrate that the properties and inhibition profiles of the human protein kinases produced in human cells are clearly differentiated from versions of the same kinase that were produced in non-human cell systems. Given the high cost and lengthy development of drugs, it would be highly justified to use human kinases with high authenticity for drug screening as well as kinase profiling. This will allow researchers to avoid pursuing false negative leads and missing promising targets.
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