Click to see all Products from IBA GmbH or a search for Publications in HighWire.

IBA GmbH is dedicated to provide high quality tools for life science research. Based on its proprietary TAGnologies (Strep-tag, His-tag, TLM-tag) IBA is a competent research partner in academia and pharma industry providing state of the art cloning, expression and protein purification technologies as well as delivery technologies for proteins and nucleic acids. The proprietary protein products and services cover the entire drug discovery process. IBA assists its customers from early discovery to the market identifying the company as prime innovator for the pharmaceutical industry meeting their need to increase research productivity.

Technical Information: Application Note & Manuals

Technical Information: Calculations

Vector Information

Product Ranges

IBA ProteinTAGnologies: Protein Expression and Purification

IBA Protein TAGnologies: Purification via tags/products

Strep-tag®, 6xHistidine-tag or double-tag products for purification, detection and assays of recombinant proteins.




The Strep-tag/Strep-Tactin system is one of the most widely used affinity chromatography systems in protein purification and detection and offers many advantages:

• Undisturbing short tag
• Rapid one-step purification under physiological conditions
• Unsurpassed purity and bioactivity

For the use of this system IBA offer a large range of high quality products, such as various Starter Kits for newcomers.

• Different Strep-Tactin purification resins and pre-packed columns
• Detection Systems with Strep-Tactin conjugates or a monoclonal antibody

Products

Protein purification: Strep-tag - Histidine-tag - Resins
Protein detection: Antibodies - Conjugates
Protein:protein interaction: One-STrEP-tag
Immobillization/assay/diagnostic: Microplates - Beads - Columns
Apoptosis NEW: FasL-Strep Assays

IBA Gene TAGnologies

Magnetic Assisted Transfection (MATra)




Magnet assisted Transfection is an easy-to-handle and highly efficient method to transfect cells in culture. Using this technique nucleic acids, such as plasmid DNA, oligonucleotides or siRNA, are in a first step associated with magnetic particles. Exploiting magnetic force the full nucleic acid dose is then rapidly drawn towards and delivered into the target cells leading to efficient transfection. One-STrEP Protein:Protein Interaction Analysis
The new One-STrEP system is an extremely fast and efficient method to identify protein complexes. While other systems require tedious optimisation because of high background, or two successive purification steps, the One-STrEP system requires one STEP only to isolate intact protein complexes. In this system, Strep-Tactin columns are used for fast protein purification under physiological conditions with low background, even enabling the isolation of weakly associated binding partners. Phage Coupling Reagent for NMR
Pf1 phage nuclear magnetic resonance co-solvent. In this new reagent the filamentous Pf1 phage serves as dipolar coupling reagent for NMR measurements. To measure the Dipolar Coupling Interaction is a valuable source of structural information that contains both an angle and a distance component. Pf1 can be used to induce a tunable degree of alignment of proteins or nucleic acids.

Cloning/gene expression
Classic cloning and expression vectors

• E.coli: IBA offers the Tet and T7 expression systems producing recombinant proteins in E. coli. Therefore, a variety of pASK-IBA vectors with unique characteristics is available supporting you with the purification of your protein of interest. The choice of system and vector depends on your protein and its characteristics.
• Mammalian: For expression in mammalian cells IBA has released the pEXPR-IBA vectors with CMV promotor providing strong expression in a wide range of mammalian cells.
• Yeast: For expression in yeast cells IBA now offers pKS-ST vectors with Strep-tag® and HA epitope-tag from Dual Systems
• StarGate: Combinatorial cloning and expression. Transfer any gene of interest systematically into E. coli, yeast, mammalian (etc.) background with high speed and convenience to screen for the optimal host/tag combination.

Nucleic acid specialties
IBA`s nucleic acid division specialized in e.g. RNA oligonucleotide synthesis (including RNAi), labeled oligonucleotides (e.g. for real-time PCR) and triphosphates. - Single stranded RNA-oligonucleotides, phosphorothioate (PTO-) protected - Tools for enzymatic DNA and RNA modifications.

Products

Oligonucleotides: Synthetic DNA/RNA
Cloning, PCR: StarGate
Transfection: MATra

IBA Cell TAGnologies

CD8+-T-cell staining and isolation
Reversible, antigen-specific staining and isolation of T-cells using Streptamers.

Products

T-cell staining and isolation: Streptamers

Brochures & Catalogues

Protein TAGnologies
Gene TAGnologies
StarGate combinatorial cloning and expression
Streptamer
MATra
FasL-Strep Assays
Strep-tag

Presentations

StarGate System
StrepTag

Protocols

StrepTag Protocol
StrepWell Protocol
Blotting Protocol
PCR pASK, pPR, pEXPR
SpinColumn Protocol
Reagents StrepTag
Lyse Protocol
Biacore Immobilization Protocol
StrepMAB Classic purification Protocol

Products from IBA.