Zen Bio specialises in the culture of human preadipocytes and adipocytes, providing the highest quality cells and reagents for research. The cells have multiple applications from drug screening and discovery to research in the basic biology and endocrine function of adipose tissue. The cells are performance tested for responsiveness in assays such as proliferation, lipolysis, glucose uptake and leptin secretion. Zen-Bio also has a thorough and highly effective array of contract assays that takes full advantage of their unique, high performance, human cell system. Other Visceral Preadipocytes - Primary Human Hepatocytes - RNA - Slides - Subcutaneous Cultured Adipocytes - Subcutaneous Cultured Preadipocytes - Subcutaneous Primary Adipocytes, Flash Frozen (Floaters) Other Visceral Preadipocytes
Zen-Bio has limited availability of omental preadipocytes and adipocytes. The omental adipose depot is critical to research in type 2 diabetes. Clinical studies have shown an increase in this depot specifically is strongly correlated with the onset of type 2 diabetes. Currently all cells are from high BMI donors (morbidly obese) There are a limited number of subcutaneous and omental patient matched sets. Primary Human Hepatocytes Primary human hepatocytes are available cryopreserved. Cryopreserved cells are suitable for a variety of assays including induction, toxicity and drug metabolism. Both adherent and suspension cells are available. Cells have been tested for performance in viability, ECOD, 7-HCG, and 7-HCS assays. Cells have tested negative for HIV-1, HIV-2, HTLV-1, HTLV-2, HbsAG, HbcAB, HCV, and RPR. Cells are tested for CMV some CMV positive lots are released for experiments. RNA Adipose-derived total RNA isolated from a variety of sources by a method modified slightly from that used by Chomcynzski and Sacchi SlidesFixed adipose tissue slides ready for histological staining or immunohistochemistry. Slides available from a variety of tissues including subcutaneous abdominal, visceral and breast adipose, from patients with a variety of BMI Subcutaneous Cultured Adipocytes Donors are between 20 and 60 years of age with no known health conditions which would compromise cell quality. Generally cells are available from male and female patients with body mass indices (BMI) in lean, normal and obese range. Subcutaneous Cultured PreadipocytesDonors are between 20 and 60 years of age with no known health conditions which would compromise cell quality. Generally cells are available from male and female patients with body mass indices (BMI) in lean, normal and obese range. Subcutaneous Primary Adipocytes, Flash Frozen (Floaters)Floaters, or primary adipocytes, are isolated from the initial collagenase digestion and filtered through sterile cheesecloth. Cells are placed in liquid nitrogen and then transferred to -80 centigrade.
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