• Tight regulation and high expression levels
• Grow cells in rich medium, such as YPD
• No medium change required
• Easy protein purification using the popular Strep-tag

Technology information

The KickStart protein expression kit has been specifically designed for inducible, high-level expression of recombinant proteins in Saccharomyces cerevisiae.

KickStart expression vectors combine two important features for protein expression:

• Inducible ADH2 promoter: The ADH2 promoter is induced when glucose becomes depleted in the medium. During early growth in YPD medium glucose levels are high and the ADH2 promoter is inactive - thereby preventing negative effects on yeast growth.
• Once the cells reach the early stationary phase glucose is depleted from the medium and the ADH2 promoter is induced, producing high amounts of protein.
• KanMX cassette: KickStart™ vectors feature a KanMX cassette, which allows selection with G418 in rich medium. The use of rich medium such as YPD will significantly enhance cell densities and protein yield.

What are the advantages?

• High-level, inducible expression
• No addition of reagents or change of medium needed for induction
• Tight regulation of induction allows expression of toxic proteins
• Popular Step-tag for quick and easy protein purification
• Protease deficient expression strain for higher protein yields
• Optimized extraction buffers supplied with the kit

Kit content

• pKS1 expression vector - 5 µg
• pKS1-ST expression vector - 5 µg
• pKS-ST-Gal control vector - 5 µg
• DSY-5 expression strain - stab culture
• XT extraction buffer & glass beads - 50 ml