Ultrapure nucleoside triphosphates for in vitro transcription experiments.
| Products & Ordering | ||
| NTP Mix – 25 mM Solution NU-1024 Premix of 25 mM ATP, CTP, GTP and UTP | NTP Bundle NU-1014 4 x 100 mM (ATP, CTP, GTP, UTP) | ATP – Solution NU-1010 SOL 100 mM Sodium salt solution |
| CTP – Solution NU-1011 SOL 100 mM Sodium salt solution |
GTP – Solution NU-1012 SOL 100 mM Sodium salt solution |
UTP – Solution NU-1013 SOL 100 mM Sodium salt solution |
If you require large amounts (> 100 ml, > 1 l), additional bulk discounts are available. Please contact us to receive an individual quotation.
In vitro synthesis of RNA is catalyzed by bacteriophage (SP6, T7 or T3) RNA polymerases using linear DNA as a template. Ribonucleoside triphosphates (NTPs) are the building blocks of these in vitro transcription reactions.
Since amplification sensitivity, product yield and reproducibility are strongly affected by contaminating impurities, the quality of NTPs is of considerably importance.
Each lot of Jena Bioscience’s NTP powders is confirmed free of DNase, RNase, Protease and nicking activity. Furthermore, all NTPs are functionally tested by T7 RNA Polymerase-mediated in vitro transcription.
If you require large amounts (> 100 ml, > 1 l), additional bulk discounts are available. Please contact us to receive an individual quotation.
In vitro synthesis of RNA (> 20 nt up to several thousand nt) is catalyzed by bacteriophage RNA polymerases using linear DNA as a template (in vitro transcription). T7 RNA polymerase is the most efficient and widely used RNA polymerase. A modified version (T7 P&L RNA polymerase) with proline 266 replaced by leucine (P266L) has been associated with decreased abortive transcription[1], increased 5′ homogeneity of transcripts synthesized from A-initiating phi2.5 promoter[2], increased 5′ incorporation efficiency of GTP analogs[3].
140 – 160 µg RNA are synthesized after 30 min incubation with our HighYield formulation (1 μg T7 control template, 1.4 kb RNA transcript).
| Products & Ordering | |
| HighYield T7 RNA Synthesis Kit RNT-101 RNA synthesis via in vitro transcription with T7 RNA Polymerase | HighYield T7 P&L RNA Synthesis Kit RNT-201 RNA synthesis via in vitro transcription with a modified T7 RNA Polymerase (P266L) |
[1] Guillerez et al. (2005) A mutation in T7 RNA polymerase that facilitates promoter clearance. Natl. Acad. Sci. U.S.A102:5958.
[2] Salvail-Lacoste et al. (2018) Affinity purification of T7 RNA transcripts with homogeneous ends using ARiBo and CRISPR tags. RNA19:1003.
[3] Lyon et al. (2018) A T7 RNA Polymerase Mutant Enhances the Yield of 5′-Thienoguanosine-Initiated RNAs. ChemBioChem19:142.
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