Cell4Pharma is a Contract Research Organization (CRO), offering state-of-the-art nephrotoxicity screening assays and vesicular transport assays to the pharmaceutical and biotech industries.
Cell4Pharma has developed innovative vesicle-based assays that enable precise studies of cell communication, drug delivery and membrane dynamics. These assays improve the accuracy and efficiency of early-stage research, supporting drug development and the understanding of cellular processes.
Human drug efflux transporters (ABC transporters) are transiently overexpressed in human embryonic kidney (HEK293) cells. Inside-out membrane vesicles are prepared from these HEK293 cells and used for high-throughput applications. The transporters translocate substrates into membrane vesicles in an ATP-dependent manner. Detection of the substrate inside the vesicles is possible with radiolabel, fluorescence, or LC-MS detection. Cell4Pharma delivers high quality large uniform batches with guaranteed transport ratios. Each vesicle kit contains the following materials for 100 reactions:
Kits can be used for substrate identification or inhibition screening. A master mix will be prepared, added to the membrane vesicles and incubated at 37℃ in the presence of ATP or AMP. Loaded vesicles will be filtered, lysed and eluted for detection.
| Product Name | Catalog No. |
| Human BCRP vesicle kit (HEK293 derived) | C4PVK006 |
| Human BSEP vesicle kit (HEK293 derived) | C4PVK007 |
| Human MRP1 vesicle kit (HEK293 derived) | C4PVK001 |
| Human MRP2 vesicle kit (HEK293 derived) | C4PVK002 |
| Human MRP3 vesicle kit (HEK293 derived) | C4PVK003 |
| Human MRP4 vesicle kit (HEK293 derived) | C4PVK004 |
| Human MRP5 vesicle kit (HEK293 derived) | C4PVK005 |
| Human MRP8 vesicle kit (HEK293 derived) | C4PVK008 |
| Human Pgp vesicle kit (HEK293 derived) | C4PVK010 |
| Human Pgp vesicle kit (HEK293 derived) with FluoPgp substrate | C4PVK011 |
| Human Control vesicle kit (HEK293 derived) | C4PVK009 |
Our nephrotoxicity assays, based on our patented ciPTEC human renal model, provide precise and reliable safety assessment of drug candidates, delivering comprehensive data to strengthen your drug development process. With personalised solutions, we cater to the unique needs of your projects at any stage of development.
Cell4Pharma provides vesicular transport assays with custom made HEK293-derived membrane vesicles to study your ATP-binding cassette (ABC) transporter of choice. With a commitment to producing reliable results, we offer solutions tailored to meet the specific needs of your research. Whether your focus is on substrate uptake or drug-drug interactions, our expertise in vesicular transport assays can support your research objectives.