Cal-520™ provides the most robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilisation. Cal-520™ AM is a new fluorogenic calcium-sensitive dye with a significantly improved signal to background ratio and intracellular
retention compared to the existing green calcium indicators (such as Fluo-3 AM and Fluo-4 AM). The higher signal/background ratio and better intracellular retention make the Cal-520™ calcium assay a robust tool for evaluating GPCR and calcium channel targets as well as for screening their agonists and antagonists.
AAT Bioquest’s preliminary in-house research indicated that Cal-520™ AM can be readily loaded to a guinea pig heart and stays there for a few hours in the absence of probenecid. The calcium signal can be readily monitored using Cal-520™ AM while it is difficult to observe the calcium signal under the same conditions using other green calcium dyes such as Fluo-3 AM and Fluo-4 AM.