The scope of nucleic acid-based assays continues to expand. There is no consensus on the choice of a method for nucleic acid detection. Key factors governing detection method choice include stability, sensitivity, speed and convenience, and the overall cost for the detection reagents and detection system. The reasons for ultrasensitive analysis in nucleic acid include analysis of genetic material from single cells and from rare cells, such as trophoblasts in maternal circulation, and single-copy gene detection. In addition, better detection methods may provide a route to assays that require fewer amplification cycles or eliminate the multicycle protocols for nucleic acid amplification reactions (e.g., PCR). Although many detection technologies, such as those based on chemiluminescence, may play an important role in the future, at present fluorescence is still the most widely used detection technique in analyzing nucleic acids.
DNA and RNA Quantifying Reagents
Helixyte™ Green dsDNA Quantifying Reagent (Cat# 17597) and StrandBrite™ Green RNA Quantifying Reagent (Cat# 17611) are optimized for double-stranded DNA and single-stranded RNA respectively. They have a high affinity for nucleic acids and an extremely large fluorescence enhancement upon binding nucleic acids, making it possible to directly detect minute amounts of nucleic acids in complex solutions within minutes, usually without interference from other biomolecules.