Golgi Apparatus

The Golgi apparatus is a complex of vesicles and folded membranes within the cytoplasm of most eukaryotic cells. It plays an essential role in the secretion of marcomolecus (e.g. proteins, lipids and carbohydrates) from the cell, the intracellular transport of marcomolecules for use by other organelles within the cell, and the formation of lysosomes. Cell-permeant probes that selectively stain the Golgi apparatus have applications in lipid metabolism and synthesis, and trafficking studies.


Cell Navigator™ NBD Ceramide Golgi Staining Kit


This Cell Navigator™ NBD Ceramide Golgi Staining kit provides a simple and rapid way to stain Golgi in live cells, or aldehyde-fixed cells selectively. C6 NBD Ceramide is administered to cells as a complex with bovine serum albumin (C6-NBD-Ceramide-BSA). Golgi apparatus is stained through the formation of the respective fluorescent metabolites. AAT Bioquest’s Cell Navigator™ NBD Ceramide Golgi Staining Kit provides an optimized assay method for examining the morphology of the Golgi apparatus with a fluorescence microscope.

Table 1. Kit Overview For Cell Navigator™ NBD Ceramide Golgi Staining Kit

ProbeNBD Ceramide
CounterstainHoechst 33342 (Nucleus, DAPI Filter Set)
ReadoutFluorescent imaging of Golgi complex
TargetLipids in Golgi complex
LabelNBD
Ex/Em467/538 nm
Filter SetFITC
MultiplexingYes
Live CellsYes
Fixed CellsYes
FixableYes
PlatformImaging
Unit Size100 Tests
Cat No.22570

NBD Ceramide Spectrum

Figure 1.Absorbance and emission spectra of NBD ceramide (Green) and Hoechst 3342 (blue), two components of the Cell Navigator™ NBD Ceramide Golgi Staining Kit (Cat No. 22750). NBD ceramide can be efficiently excited by the common 488 nm laser and visualized with a FITC filter set. Hoechst 33342 can be efficiently excited by the common 350 nm laser and visualized with a DAPI filter set.

Product ordering information


 

Table 2. Ordering Info for Cell Navigator Golgi Staining Products

Cat#Product NameUnit Size
22750Cell Navigator™ NBD Ceramide Golgi Staining Kit *Green Fluorescence*100 Tests

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