Golgi Apparatus

The Golgi apparatus is a complex of vesicles and folded membranes within the cytoplasm of most eukaryotic cells. It plays an essential role in the secretion of marcomolecus (e.g. proteins, lipids and carbohydrates) from the cell, the intracellular transport of marcomolecules for use by other organelles within the cell, and the formation of lysosomes. Cell-permeant probes that selectively stain the Golgi apparatus have applications in lipid metabolism and synthesis, and trafficking studies.

Cell Navigator™ NBD Ceramide Golgi Staining Kit

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This Cell Navigator™ NBD Ceramide Golgi Staining kit provides a simple and rapid way to stain Golgi in live cells, or aldehyde-fixed cells selectively. C6 NBD Ceramide is administered to cells as a complex with bovine serum albumin (C6-NBD-Ceramide-BSA). Golgi apparatus is stained through the formation of the respective fluorescent metabolites. AAT Bioquest’s Cell Navigator™ NBD Ceramide Golgi Staining Kit provides an optimized assay method for examining the morphology of the Golgi apparatus with a fluorescence microscope.

Figure 1.Absorbance and emission spectra of NBD ceramide (Green) and Hoechst 3342 (blue), two components of the Cell Navigator™ NBD Ceramide Golgi Staining Kit (Cat No. 22750). NBD ceramide can be efficiently excited by the common 488 nm laser and visualized with a FITC filter set. Hoechst 33342 can be efficiently excited by the common 350 nm laser and visualized with a DAPI filter set.