iFluor™ secondary detection reagents are optimized for use in a wide range of fluorescence-based applications, including cell-imaging, flow cytometry, and Western blotting. iFluor™ conjugates are produced with the highest quality secondary antibodies and streptavidins, and subjected to rigorous quality control assays. To ensure customers receive products with the greatest sensitivity and selectivity, iFluor™ secondary antibodies have been affinity purified and cross-adsorbed to minimize cross-reactivity.
iFluor Secondary Reagents
- iFluor™ Secondary Antibodies
- iFluor™ Streptavidin Conjugates
- iFluor™ Styramide Conjugates for Enzyme Amplification
- PSA™ Secondary Detection Signal Amplification Kits
- Also available are HRP, biotin and other small organic dyes labeled secondary antibodies and streptavidin conjugates
Photostability Comparison
Conjugates labeled with iFluor™ dyes exhibit superior brightness, higher signal-to-noise ratios and improved photostability, outperforming spectrally similar secondary antibody conjugates such as fluorescein and most Alexa Fluor® secondary antibody conjugates. The excellent photostability and pH-insensitive fluorescence of our iFluor™ secondaries, allows researchers more time for capturing images (Figure 1 and Figure 2).
Figure 1. Comparison of fluorescence intensity between HeLa cells labeled with GAM IgG-FITC and GAM IgG-iFluor™ 488.
Figure 2. Comparison of photobleaching rates of iFluor™ 488 goat anti-mouse IgG and the conventional fluorescein (FITC) goat anti-mouse IgG. Tubulin of HeLa cells was labeled with (top series) iFluor™ 488 GAM-IgG or (bottom series) FITC GAM-IgG. Images were taken at 60-second intervals on a Keyence X710 fluorescence microscope. TFI represents total fluorescence intensity as calculated by ImageJ software.
iFluor™ Secondary Antibody Product Information
| Host Species | Goat | ||||||||
| Target Reactivity | Anti-mouse IgG | Anti-mouse IgG *Cross-Adsorbed* | Anti-Rabbit IgG | Anti-Rabbit IgG *Cross-Adsorbed* | |||||
| Label | Ex/Em (nm) | 200 µg | 1 mg | 200 µg | 1 mg | 200 µg | 1 mg | 200 µg | 1 mg |
| iFluor™ 350 | 344/448 | 16440 | 16730 | 16520 | 16770 | 16600 | 16795 | 16670 | 16825 |
| iFluor™ 405 | 402/425 | 16444 | 16731 | 16524 | 16771 | 16604 | 16796 | 16674 | 16826 |
| iFluor™ 488 | 491/516 | 16448 | 16735 | 16528 | 16773 | 16608 | 16800 | 16678 | 16828 |
| iFluor™ 514 | 527/554 | 16452 | 16736 | 16532 | 16774 | 16612 | 16801 | 16682 | 16829 |
| iFluor™ 532 | 543/563 | 16456 | 16737 | 16536 | 16775 | 16616 | 16802 | 16686 | 16830 |
| iFluor™ 546 | 541/557 | 16457 | 16537 | 16618 | 16688 | ||||
| iFluor™ 555 | 556/569 | 16460 | 16739 | 16540 | 16776 | 16620 | 16803 | 16690 | 16831 |
| iFluor™ 568 | 568/587 | 16462 | 16541 | 16622 | 16692 | ||||
| iFluor™ 594 | 587/603 | 16468 | 16741 | 16548 | 16780 | 16628 | 16806 | 16698 | 16833 |
| iFluor™ 633 | 638/652 | 16478 | 16743 | 16558 | 16782 | 16638 | 16808 | 16704 | 16835 |
| iFluor™ 647 | 654/669 | 16482 | 16744 | 16562 | 16783 | 16642 | 16809 | 16710 | 16837 |
| iFluor™ 680 | 683/700 | 16486 | 16745 | 16566 | 16784 | 16646 | 16810 | 16712 | 16838 |
| iFluor™ 700 | 690/713 | 16494 | 16746 | 16574 | 16785 | 16652 | 16811 | 16714 | 16839 |
| iFluor™ 750 | 756/777 | 16506 | 16748 | 16586 | 16788 | 16660 | 16813 | 16720 | 16842 |
| iFluor™ 790 | 786/811 | 16507 | 16750 | 16587 | 16790 | 16661 | 16815 | 16721 | 16843 |
| Unit Size | 200 µg | 1 mg | 200 µg | 1 mg | 200 µg | 1 mg | 200 µg | 1 mg | |
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