Accessory Reagents

Bangs Laboratories offers a variety of accessory products for use in microparticle reagent development. The collection includes crosslinking reagents and surfactants, as well as solutions and buffers for microsphere coating and storage. These products complement our extensive catalog of polymer, silica, and magnetic microparticles, and also offer a convenient means to replenish individual components of coupling kits.

Bangs Bead Coupling Buffers are ready-to-use buffers that are available in a variety of pH levels (4.5 to 9.0). They can be used as coupling or wash buffers for plain, dyed, or functionalized polymer microspheres. Our Bangs Bead Storage Buffer is available in two pH levels: 7.4 and 8.5.

Our Bead Solution is a ready-to-use aqueous suspending solution for the dilution and/or storage of your uncoated plain, dyed, or functionalized polymer microspheres (both carboxylated or aminated). An antimicrobial agent deters microbial contamination, and stabilizers promote suspension dispersity, peace of mind, and harmonious accord in the laboratory. For general information, or to make your own, see our TSD 0300 Buffers.

Catalog Number Description pH Volume (mL)
BUFF1 Bangs Bead Coupling Buffer 4.5 250, 500, 1000, 2000
BUFF2 Bangs Bead Coupling Buffer 6.0 250, 500, 1000, 2000
BUFF3 Bangs Bead Coupling Buffer 7.4 250, 500, 1000, 2000
BUFF4 Bangs Bead Coupling Buffer 9.0 250, 500, 1000, 2000
BUFF5 Bangs Bead Storage Buffer 7.4 250, 500, 1000, 2000
BUFF6 Bangs Bead Storage Buffer 8.5 250, 500, 1000, 2000
SOLN1 Bangs Bead Solution 500, 1000, 2000

Microspheres are routinely coated with ligands such as antibodies, oligonucleotides, and peptides, for use in diagnostics and bioseparations. Carboxyl- and amine-modified microspheres require the use of a chemical linker for activation and covalent immobilization of ligand. DEPC-Carbodiimide (EDAC) and Glutaraldehyde are two of the most common crosslinkers used in bead coating protocols. To purchase as a kit, see our coupling kits.

EDAC is a zero-length crosslinker that is routinely used for the covalent binding of amine-containing ligands to carboxylated microspheres.

Glutaraldehyde is a homobifunctional linker that is suitable for binding amine-containing ligands to amine-modified beads.

Catalog Number Description Volume
AA010-1 DEPC – Carbodiimide (EDAC) 1g
AA010-5 DEPC – Carbodiimide (EDAC) 5g
AA012 Glutaraldehyde, EM Grade, 25% 10 × 10mL (ampoules)


Trypan blue exclusion is a common method for the determination of cell viability. It is used extensively in biopharmaceutical cell culture programs, and for a range of research studies including apoptosis, cytopathic effects of viral infection, and effects of sample processing methods on cell viability and concentration. Our solution is suitable for traditional manual staining and hemocytometer counting. NOTE: This product is not intended to be a plug-in replacement bottle for automated cell viability analyzers. Instrument manufacturers please contact us for bulk/OEM packaging if this is your interest.
Catalog Number Description Volume (mL)
AA020 Trypan Blue 0.4% Solution 125mL
AA021 Trypan Blue 0.4% Solution 250mL


  • Louis KS, Siegel AC. (2011) Cell viability analysis using trypan blue: manual and automated methods. Methods Mol Biol; 740:7-12.
  • Phelan K, May KM.(2017) Mammalian Cell Tissue Culture Techniques. Curr Protoc Mol Biol.5;117:A.3F.1-A.3F.23

Surfactants are commonly utilized with microspheres composed of hydrophobic polymer matrices such as polystyrene to facilitate bead wetting and to deter or treat aggregation. Common concentrations are ~0.1% for uncoated polymer beads and ≤0.05% in the storage buffer of coated polymer, magnetic, and silica beads.

Triton™ X-100 and Tween® 20 are nonionic surfactants used in the storage buffers of coated bead suspensions, while Sodium Dodecyl Sulfate (SDS) is an anionic surfactant that decreases hydrophobicity and participates in charge stabilization of the suspension.

Catalog Number Description Unit Size
AA014 Triton™ X-100 Nonionic Surfactant 10
AA016 Tween® 20 Nonionic Surfactant 10
AA018 Sodium Dodecyl Sulfate Anionic Surfactant 10


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