Bangs Laboratory’s Quantum™ MESF kits include microsphere populations that are ~7-9µm in diameter: one blank population and a range of labeled bead populations with increasing amounts of fluorochrome. The assignment of fluorescence intensity in Molecules of Soluble Fluorochrome (MESF) units is performed through direct comparison of fluorescence measurements from solutions of the pure fluorochrome with those from microspheres surface-labeled with the same fluorochrome. In addition to quantitative fluorescence cytometry, Quantum™ MESF kits may be used to assess the detection threshold, resolution, and linearity of the instrument. A free QuickCal® analysis template is provided with each kit to aid in determining the expression level of cells, and for evaluating instrument linearity and detection threshold. Quantum™ MESF kits are available in three sizes: A (20 tests), B (100 tests), and C (280 tests).
Note to FITC MESF Users: We are now offering a single version of Quantum™ FITC MESF rather than the separate Low, Medium, and High Intensity kits. The new version consists of one blank and five labeled bead populations that span the full intensity range previously covered by the three separate kits. The expiration date on Quantum™ FITC-5 MESF kits is six months from the date of purchase; all other kits have twelve month expiration dates.
For additional information regarding Bangs Laboratory’s Quantum™ MESF products, please see PDS 818, Quantum™ Simply Cellular® & Quantum™ MESF Tips and Techniques. For details regarding QuickCal® templates, please see PDS 819, QuickCal® v. 2.3 Data Analysis Program.