Superparamagnetic particles have been utilized extensively in diagnostics and other research applications for the purification of cells and biomolecules, such as antibodies, nucleic acids, and polypeptides. They confer a number of benefits, including ease of separation and suitability for automation. When coated with recognition molecules, magnetic microspheres are ideal for the efficient capture and separation of target. Unwanted sample constituents may be washed away following a simple magnetic separation step.
Magnetic Isolation Products & Kits
BioMag® and BioMag®Plus are high-performance superparamagnetic particles that are uniquely suited to cell separations. We offer a full range of particles coated with anti-CD antibodies, secondary antibodies, and Fc-binding proteins. See TSD 528, BioMag® and Cell Sorting.
BIOMAG ANTI-LEUKOCYTE FOR MOUSE
BioMag® anti-Leukocyte magnetic particles are coated with anti-CD antibodies for the positive selection of specific cell subsets from purified leukocyte preps or whole blood. Positive selections permit rapid and efficient isolation of target cell populations.
| Catalog Number | Description | Concentration (mg /mL) | Volume (mL) |
| BM592 | BioMag® anti-Mouse CD4 | 1 | 5 |
| BM593 | BioMag® anti-Mouse CD8a | 1 | 5 |
| BM594 | BioMag® anti-Mouse CD45R | 1 | 5 |
REFERENCES
- Grafton M, Maleki T, Zordan M, Reece L, Byrnes R, Jones A, Todd P, Leary J (2011) Microfluidic MEMS hand-held flow cytometer. Proc. SPIE 7929, Microfluidics, BioMEMS, and Medical Microsystems IX, 79290C (BioMag anti-CD45)
BIOMAG ANTI-LEUKOCYTE FOR HUMAN
BioMag® anti-Leukocyte magnetic particles are coated with anti-CD antibodies for the positive selection of specific cell subsets from purified leukocyte preps or whole blood. Positive selections permit rapid and efficient isolation of target cell populations.
| Catalog Number | Description | Concentration (mg /mL) | Volume (mL) |
| BM595 | BioMag® anti-Human CD2 | 4 | 5 |
| BM583 | BioMag® anti-Human CD8 | 1.5 | 5 |
| BM596 | BioMag® anti-Human CD11b | 1 | 5 |
| BM584 | BioMag® anti-Human CD14 | 1.5 | 5 |
| BM587 | BioMag® anti-Human CD34 | 4 | 5 |
| BM588 | BioMag® anti-Human CD45 | 4 | 5 |
| BM589 | BioMag® anti-Human CD56 | 4 | 5 |
| BM590 | BioMag® anti-Human CD71 | 4 | 5 |
BIOMAG® FOR ANTIBODY LABELED CELLS
BioMag® secondary antibody magnetic particles are used extensively in the capture of immune complexes for immunoprecipitation and separation of primary antibody-labeled cells. Secondary antibody coatings also afford a convenient method for binding specific primary antibodies to BioMag® particles. BioMag®Plus Mouse anti-Fluorescein IgG particles may be used to isolate fluorescein-tagged species such as labeled cells or nucleic acids.
| Catalog Number | Description | Concentration (mg / mL) | Volume (mL) |
| BM563 | BioMag® Goat anti-Human IgG | 1 | 50 |
| BM562 | BioMag® Goat anti-Human IgG (Fc Specific) | 5 | 50 |
| BM561 | BioMag® Goat anti-Human IgM | 1 | 50 |
| BM549 | BioMag® Goat anti-Mouse IgG | 1 | 50 & 500 |
| BM550 | BioMag® Goat anti-Mouse IgG (Fc Specific) | 1 | 50 & 500 |
| BP619 | BioMag®Plus Goat anti-Mouse IgG | 1 | 50 |
| BP612 | BioMag®Plus Goat anti-Mouse IgG Antibody Coupling Kit | 1 | 25 |
| BM558 | BioMag® Goat anti-Mouse IgM | 1 | 50 & 500 |
| BM559 | BioMag® Goat anti-Rabbit IgG | 1 | 50 & 500 |
| BM560 | BioMag® Goat anti-Rat IgG | 1 | 50 & 500 |
| BM548 | BioMag® Goat anti-Rat IgG (Fc specific) | 5 | 50 & 500 |
| BM557 | BioMag® Goat anti-Rat IgM | 5 | 50 & 500 |
| BP622 | BioMag®Plus Mouse anti-Fluorescein IgG | 1 | 50 |
BIOMAG® PROTEIN A AND BIOMAG® PROTEIN G
BioMag® Protein A and BioMag® Protein G may be used to conduct Fc-mediated separations. See a reference chart of antibody affinities here.
| Catalog Number | Description | Concentration (mg/mL) | Volume (mL) |
| BM554 | BioMag® Protein A | 5 | 2, 10 |
| BP620 | BioMag®Plus Protein A | 5 | 2, 10 |
| BM553 | BioMag® Protein G | 5 | 2, 10 |
| BP627 | BioMag®Plus Protein G | 5 | 2, 10 |
BIOMAG® PROTEIN A & G
BioMag® particles with affinity binding proteins may be coated with the primary antibody or biotinylated molecule of interest through a straightforward incubation. They are also suitable for off-the-shelf use in applications, such as immunoprecipitation or PCR product clean-up.
| Catalog Number | Description | Concentration (mg / mL) | Volume (mL) |
| BM554 | BioMag® Protein A | 5 | 2 & 10 |
| BP620 | BioMag® Plus Protein A | 5 | 2 & 10 |
| BP614 | BioMag® Plus Protein A Antibody Isolation Kit | 5 | 2.5 (5 reactions x 0.5mL) |
| BM553 | BioMag® Protein G | 5 | 2 & 10 |
| BP627 | BioMag® Plus Protein G | 5 | 2 & 10 |
| BP626 | BioMag® Plus Protein G Antibody Isolation Kit | 5 | 2.5 (5 reactions x 0.5mL) |
BIOMAG® KITS
With their tremendous surface area, BioMag® particles are ideal for purifications. Our antibody isolation kits provide a means for the efficient isolation of antibody from serum or cell culture supernatant, and our protein removal kits permit the depletion of ubiquitous proteins, such as albumin and immunoglobulin from serum, for a significant improvement in resolution of less abundant proteins.
| Catalog Number | Description | Number of Reactions |
| BP614 | BioMag®Plus Protein A Antibody Isolation Kit | 5 × 5µL serum or cell culture supernatant |
| BP626 | BioMag® Plus Protein G Antibody Isolation Kit | 5 × 5µL serum or cell culture supernatant |
| BP658 | BioMag® ProMax Albumin Removal Kit | 25 × 10µL serum |
| BP659 | BioMag® ProMax Serum IgG Removal Kit | 10 × 1µL serum |
BIOMAG® SECONDARY AB
BioMag® secondary antibody particles are used extensively in the capture of immune complexes for immunoprecipitation and antibody-labeled cells for indirect cell separation. Secondary antibody coatings also afford a convenient method for binding specific primary antibodies to BioMag® particles.
| Catalog Number | Description | Concentration (mg / mL) | Volume (mL) |
| BM563 | BioMag® Goat anti-Human IgG | 1 | 50 |
| BM562 | BioMag® Goat anti-Human IgG (Fc Specific) | 5 | 50 |
| BM561 | BioMag® Goat anti-Human IgM | 1 | 50 |
| BM549 | BioMag® Goat anti-Mouse IgG | 1 | 50 & 500 |
| BM550 | BioMag® Goat anti-Mouse IgG (Fc Specific) | 1 | 50 & 500 |
| BP619 | BioMag®Plus Goat anti-Mouse IgG | 1 | 50 |
| BP612 | BioMag®Plus Goat anti-Mouse IgG Antibody Coupling Kit | 1 | 25 |
| BM558 | BioMag® Goat anti-Mouse IgM | 1 | 50 & 500 |
| BM559 | BioMag® Goat anti-Rabbit IgG | 1 | 50 & 500 |
| BM560 | BioMag® Goat anti-Rat IgG | 1 | 50 & 500 |
| BM548 | BioMag® Goat anti-Rat IgG (Fc specific) | 5 | 50 & 500 |
| BM557 | BioMag® Goat anti-Rat IgM | 5 | 50 & 500 |
Our protein removal kits permit the depletion of ubiquitous proteins, such as albumin and immunoglobulin from serum, for a significant improvement in resolution of less abundant proteins.
REFERENCES
- Chade AR, Zhu XY, Krier JD, Jordan KL, Textor SC, Grande JP, … & Lerman LO. (2010) Endothelial progenitor cells homing and renal repair in experimental renovascular disease. Stem cells, 28(6), 1039-1047. (ProMax Albumin Removal Kit)
- MORI, M., ICHIBANGASE, T., YAMASHITA, S., KIJIMA-SUDA, I., KAWAHARA, M., & IMAI, K. (2015). Quantification of horse plasma proteins altered by xylazine using the fluorogenic derivatization-liquid chromatography-tandem mass spectrometry. Journal of equine science, 26(4), 141-146. (ProMax Albumin Removal Kit)
- Perez P, Adriaansen J, Goldsmith CM, Zheng C, & Baum BJ. (2011) Transgenic α‐1‐antitrypsin secreted into the bloodstream from salivary glands is biologically active. Oral diseases, 17(5), 476-483. (ProMax Albumin Removal Kit)
- Cretich, M., Di Carlo, G., Giudici, C., Pokoj, S., Lauer, I., Scheurer, S., & Chiari, M. (2009). Detection of allergen specific immunoglobulins by microarrays coupled to microfluidics. Proteomics, 9(8), 2098-2107. (ProMax IgG Removal Kit)
Our Con A- and WGA coated BioMag®Plus microparticles provide a convenient means for isolating glycoproteins from serum or cell lysate, and for investigating other lectin/glycan-mediated processes. The BioMag®Plus magnetic particle format provides high surface area, and permits easy and efficient separations. For more information about using our BioMag Con A, see TSD 0028: Con A.
REFERENCES
- Lee Y, Block G, Chen H, Folch-Puy E, Foronjy R, Jalili R,… Lin SH, (2008) One-step isolation of plasma membrane proteins using magnetic beads with immobilized concanavalin A, Protein Expr Purif., V62(2):223-229
- Sparbier K, Koch S, Kessler I, Wenzel T, Kostrzewa M. (2005) Selective Isolation of Glycoproteins and Glycopeptides for MALDI-TOF MS Detection Supported by Magnetic Particles. J Biomol Tech 16(4):407-413
- Skene, P. J., & Henikoff, S. (2017). An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites. eLife, 6, e21856. (BP531, BioMag Concanavalin A)
- Liu, N, Hargreaves, V.V, Zhu, Q., Kurland, JV, Hong, J, Kim, W, … & Nakamura, Y. (2018) Direct Promoter Repression by BCL11A Controls the Fetal to Adult Hemoglobin Switch. Cell. (BP531, BioMag Concanavalin A)
- Skene, PJ, Henikoff, JG, & Henikoff, S (2018). Targeted in situ genome-wide profiling with high efficiency for low cell numbers. Nature protocols, 13(5), 1006.(BP531, BioMagPlus Con A)
- Skene, PJ, & Henikoff, S. (2017). CUT&RUN: Targeted in situ genome-wide profiling with high efficiency for low cell numbers. bioRxiv, 193219.(BP531, BioMagPlus Con A)
- Janssens DH, Wu SJ, Sarthy JF, Meers MP, Myers CH, Olson JM, … & Henikoff, S. (2018) Automated in situ profiling of chromatin modifications resolves cell types and gene regulatory programs. bioRxiv, 418681. (BP531, BioMagPlus Con A)
Meet Magnefy™, our ~1µm high-performance superparamagnetic microspheres. As high surface area / high surface titer microparticles with a rapid separation profile, Magnefy offer a performance-driven solid phase for magnetic particle-based applications, including SPRI-based total DNA isolation (COOH), and molecular- and immunoassays. Contact us today to explore using Magnefy as your OEM magnetic SPRI bead. See Magnefy™ New Product Development: Magnetic Particle Characterization & QC Testing
| Catalog Number | Description |
| MFY0002 | Magnefy™ • COOH 1µm |
| MFYS1N | Magnefy™ • SA 1µm |
REFERENCES
- Meyer M, Stenzel U, Hofreiter, M. (2008). Parallel tagged sequencing on the 454 platform. Nature Protoc; 3(2), 267.
- Hawkins TL, O’Connor-Morin T, Roy A, Santillan C. (1994). DNA purification and isolation using a solid-phase. Nucleic Acids Res; 22(21), 4543-4.
SPECIFIC AND NON-SPECIFIC (TOTAL) NUCLEIC ACID ISOLATION
BioMag® and ProMag™ products support specific and non-specific (total) nucleic acid isolation through the immobilization/capture of biotinylated oligos and the purification of mRNA or total DNA.
BIOMAG® STREPTAVIDIN / BIOTIN MAGNETIC PARTICLES
Streptavidin-coated magnetic particles have been used for the isolation of biotinylated PCR amplicons. The irregular morphology of BioMag® offers high binding capacities and efficient isolations.
| Catalog Number | Description | Concentration (mg /mL) | Volume (mL) |
| BM568 | BioMag® Streptavidin, Nuclease-free | 1 | 10 & 25 |
| BM551 | BioMag® Streptavidin | 5 | 5 & 50 |
| BP628 | BioMag®Plus Streptavidin | 5 | 10 |
| BP621 | BioMag®Plus Streptavidin / Biotin Binding Kit | 5 | 5 |
PROMAG® HP & PROMAG® STREPTAVIDIN
ProMag® are highly uniform polymer-based magnetic spheres available in diameters of 1µm and 3µm. A unique surface means low nonspecific binding in protein-based systems, and superior handling without the use of surfactant. These high-binding beads are suitable for use across a range of research and diagnostic applications, whether you’re working at laboratory scale or have the more stringent requirements of high throughput applications. ProMag® Streptavidin are supplied as ~1% solids (w/v) aqueous suspensions.
| Catalog Number | Description |
| PMS3HP | ProMag® HP 3 Series • Streptavidin |
| PMS1N | ProMag® 1 Series • Streptavidin |
| PMS3N | ProMag® 3 Series • Streptavidin |
MAGNETIC PARTICLE SAMPLER PACKS
Our many magnetic particle products uniquely address a wide range of applications in the life sciences, including cell isolations, affinity purifications, immunoassays, molecular assays. For development efforts, our Magnetic Particle Sampler Packs allow you to test different particles to find which yield optimal performance characteristics in your specific system.
| Catalog Number | Description |
| 21950 | Streptavidin Magnetic Sampler Pack MFYS1N, Magnefy 1µm SA – 1mL (1% solids) PMS1N, ProMag 1 Series SA – 1mL (1% solids) PMS3HP, ProMag HP 3μm SA – 1mL (1% solids) BP628, BioMagPlus SA – 2mL (5mg / mL) |
BIOMAG® MRNA PURIFICATION
Nuclease-free suspensions of BioMag® with covalently-bound Oligo dT(20) are used for the efficient isolation of polyadenylated mRNA from total RNA, tissue or cell lysate.
| Catalog Number | Description | Concentration (mg / mL) | Volume (mL) | Number Of Reations |
| BM569 | BioMag® mRNA Purification System | 5 | 2 | 20 × 100µg total RNA; or 10 × 50 – 100 mg tissue; or 10 × lysate from 5 × 106 cells |
| BM529 | BioMag® Oligo dT (20) | 5 | 2 | 20 × 100µg total RNA |
BIOMAG® DEXTRAN-COATED CHARCOAL
BioMag® Dextran-coated Charcoal is suitable for the adsorption of free tritium-labeled analyte in typical competitive radioimmunoassays. BioMag® particles are coated with NORIT® activated carbon and dextran.
| Catalog Number | Description | Concentration (mg / mL) | Volume (mL) |
| BM555 | BioMag® Dextran – coated Charcoal | 5 | 100 |
| BM556 | BioMag® Dextran – coated Charcoal Concentrate | 50 | 1000 |
REFERENCES
- Li H, Zhang L, Cui Y, Luo X, Xue C, Wang S,… Han X (2014), Characterization of recombinant Zea mays transglutaminase expressed in Pichia pastoris and its impact on full and non-fat yoghurts. J Sci Food Agric, 94:1225–1230
- Herbert V, Lau KS, Gottlieb CW, Bleicher SJ (1965) Coated charcoal immunoassay of insulin. J Clin Endocrinol Metab. 1965 Oct;25(10):1375-84.
- Keane PM, Pearson J, Walker WH (1968) Dextran-coated charcoal immunoassay of insulin. Diabetologia. 1968 Dec;4(6):339-44.
Superparamagnetic particles have been used extensively in diagnostics and other research applications for the isolation of cells and biomolecules such as antibodies, nucleic acids, and polypeptides. The biomagnetic separators available from Bangs accommodate a complete range of magnetic separation applications, including cell sorting, mRNA and DNA isolation, and the purification of proteins and protein complexes.
Our biomagnetic separators feature high-energy neodymium-iron-boron [Nd-Fe-B] magnets that ensure fast separation rates, excellent wall or well retention, and maximum yield. They are suitable for use with each of our magnetic particle lines. See our Biomagnetic Separators brochure.
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