SARS-CoV-2 (2019-nCoV) is a new type of bat coronavirus identified as the cause of an outbreak of respiratory illness first detected in Wuhan, China. Early on, many of the patients in the outbreak in Wuhan, China reportedly had some link to a large seafood and animal market, suggesting animal-to-person spread. However, a growing number of patients reportedly have not had exposure to animal markets, indicating person-to-person spread is occurring. At this time, it’s unclear how easily or sustainably this virus is spreading between people.
The coronavirinae family consists of four genera based on their genetic properties, including genus Alphacoronavirus, genus Betacoronavirus, genus Gammacoronavirus, and genus Deltacoronavirus. Host range and tissue tropism show a lot of variation among different CoVs. Generally, the Alphacoronavirus and Betacoronavirus can infect mammals, and the Gammacoronavirus and Deltacoronavirus can infect birds, but some of them can also infect mammals. SARS-CoV-2 (2019-nCoV), SARS coronavirus and MERS coronavirus all belong to the Betacoronavirus genus and are zoonotic pathogens that can cause severe respiratory diseases in humans.
Genome analysis of SARS-CoV-2 (2019-nCoV) showed that SARS-CoV-2 (2019-nCoV) was slightly different from SARS CoV and MERS CoV, but the functionally important ORFs, ORF1a and ORF1b, and major structural proteins such as the spike (S), membrane (M), envelop (E) and nucleic capsid (N) proteins are well annotated. Four structural proteins are essential for virion assembly and infection of CoVs. Homotrimers of S proteins make up the spike on the surface of virus particles and it is the sole viral membrane protein responsible for cell entry. It binds to the receptor on the target cell and mediates subsequent virus-cell fusion. It is also the key target for vaccine design. The M protein has three transmembrane domains and shapes the virions, promotes membrane curvature, and binds to the nucleocapsid. The E protein plays a role invirus assembly and release, and it is required for pathogenesis. The N protein contains two domains, both of them can bind virus RNA genome via different mechanisms. It is reported that N protein is an antagonist of interferon and viral encoded repressor (VSR) of RNA interference (RNAi), which benefit the viral replication.
SARS-CoV-2 (2019-nCoV) spike protein, which belongs to class I virus fusion protein, contains two subunits, S1 and S2. S1 mainly contains receptor binding domain (RBD), which is responsible for identifying cell receptors. S2 contains the basic elements needed for the membrane fusion process. It has been reported that SARS-CoV-2 (2019-nCoV) spike protein interacts with human ACE2 to infect human respiratory epithelial cells.
Creative Diagnostics now can provide recombinant SARS-CoV-2 (2019-nCoV) spike, nucleocapsid antigens. These antigens can be used for scientific research, detection of novel coronavirus and preparation of neutralizing antibodies. All of our antigens are produced using a standardized production process to ensure the highest quality and are performance guaranteed for the applications listed on the detailed datasheets.
*Highly recommended for the SARS-CoV-2 antibody lateral flow immunoassay development.
Human ACE2 Expressing Stable Cell Line
||Human ACE2 Expressing Stable Cell Line
This cell line is constructed by transduction of human angiotensin I converting enzyme 2 (ACE2) into HEK293T cells, followed by stable cell selection. HEK293T is derived from HEK293 and is commonly used in scientific research. HEK293T-human ACE2 cell line can be used for in vitro screening and characterization of drug candidates against SARS-CoV, SARS-CoV-2 (2019-nCoV).