Cleavage Under Targets and Tagmentation (CUT&Tag) assays use a fusion of protein A, protein G, and Tn5 (pAG-Tn5) to selectively target antibody-labelled chromatin for high-resolution sequencing analysis. These innovations enable low-cost mapping from small numbers of cells – poising CUT&Tag to enable epigenomic studies of unprecedented breadth and scale.
EpiCypher is a leading provider of user-friendly CUT&Tag reagents and workflows. The CUTANA™ CUT&Tag Kit is the result of our rigorous optimization efforts and has been intentionally designed for rapid throughput and accessible pricing. Advantages include:
CUTANA™ CUT&Tag maps histone PTMs at active regulatory elements (H3K4me1) and promoters (H3K4me3), constitutive (H3K9me3) and facultative (H3K27me3) heterochromatin and active gene bodies (H3K36me2/me3) in K562 cells. CUT&Tag is not recommended for chromatin-associated proteins.
The CUTANA™ CUT&Tag protocol generates data with high signal over background using as few as 10,000 cells and only 5-8 million sequencing reads.
These advances are driven by EpiCypher’s one-tube CUT&Tag workflow, which minimizes sample loss and increases throughput. Combined with low sequencing depth requirements, our kit provides substantial cost savings vs. ChIP-seq.
EpiCypher scientists have developed a user-friendly protocol that is highly consistent across assay replicates. The CUTANA™ CUT&Tag Kit comes with all the controls and guidance you need for successful experiments:
The CUTANA™ CUT&Tag Kit was used to map H3K4me3 in native (unfixed) nuclei, native cells, and lightly cross-linked nuclei, using protocol adaptations outlined in the manual. Results are consistent across sample preparations, expanding the application of CUT&Tag to additional sample types and targets.
The CUTANA™ CUT&Tag Manual is a complete guide to CUT&Tag experiments. It leverages EpiCypher’s own research & development to help users plan and execute successful CUT&Tag assays. In addition to our user-friendly protocol, we provide key guidance on:
The kit also comes with a two-sided protocol quick card to keep at your bench while performing CUT&Tag assays.