Our early efforts in developing SNAP-ChIP technology focused on defining histone PTM antibody specificity3 and technical monitoring of ChIP experiments (shown above). However, there are other applications for this dynamic platform, including quantitative normalization of ChIP experiments3, 4. EpiCypher is currently working to create more robust and streamlined SNAP-ChIP workflows for ChIP normalization. Testing of these protocols is underway…stay tuned for the next blog post on this topic!
1. Orlando DA, et al. Quantitative ChIP-Seq normalization reveals global modulation of the epigenome. Cell Rep, 2014. 9(3): p. 1163-70. (PubMed PMID: 25437568)
2. Egan B, et al. An Alternative Approach to ChIP-Seq Normalization Enables Detection of Genome-Wide Changes in Histone H3 Lysine 27 Trimethylation upon EZH2 Inhibition. PLoS One, 2016. 11(11): p. e0166438. (PubMed PMID: 27875550)
3. Shah RN, et al. Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies. Mol Cell, 2018. 72(1): p. 162-77 e7. (PubMed PMID: 30244833) (PMC6173622)
4. Grzybowski AT, et al. Calibrating ChIP-Seq with Nucleosomal Internal Standards to Measure Histone Modification Density Genome Wide. Mol Cell, 2015. 58(5): p. 886-99. (PubMed PMID: 26004229) (PMC4458216)