Lipid rafts are important subcellular structures rich in cholesterol and sphingolipid and signaling proteins. Lipid rafts play a critical role in cellular transport and signal transduction. Traditional method of lipid raft isolation is tedious and time consuming. There are limited numbers of commercial kits for isolation of this important structure.
Invent Biotechnologies Comparisions
Comparison of Major Lipid Raft Isolation Kits
| Brand/manufacturer | Brand# 1 | Invent Biotech |
| Product Name | Caveolae/Rafts Isolation Kit | MinuteTM Total Lipid Raft Isolation Kit for Mammalian Cells/Tissues |
| Mechanism of Isolation | Ultracentrifugation flotation | Spin column-based - floatation with a table top centrifuge |
| Average sample size | Cells (20-50 million) | Tissue (35 mg) Cells (20-30 million) |
| Number of buffers and reagents used | 6 | 3 |
| Buffer preparation prior to use | Required | Not Required |
| Density gradient formation | Required | Not Required |
| Ultracentrifugation | Required | Not Required |
| Purity of Isolated Lipid Rafts | Total Detergent Resistant lipid Rafts | Total Detergent Resistant lipid Rafts |
| Approximate Protocol Time | >5 h | 1 h |
Comparison of Major Lysosome Isolation Kits
Lysosomes are membranous vesicles that are rich in hydrolytic enzymes capable of breaking down proteins, nuclei acids, carbohydrates and other cellular components. There are several commercial kits available for lysosome isolation. However, by mechanism of isolation, there are only two types of kits: traditional density gradient centrifugation-based and novel spin column-based.
| Brand/manufacturer | Brand# 1 | Brand# 2 | Brand# 3 | Invent Biotech |
| Product Name | Lysosome Isolation Kit from Tissue and Cultured Cells | Lysosome Isolation Kit | Lysosome Enrichment Kit for Tissue and Cultured cells | MinuteTM Lysosome Isolation Kit |
| Mechanism of Isolation | Density gradient centrifugation | Density gradient centrifugation | Density gradient centrifugation | Spin column-based precipitation |
| Average sample size | Tissue (100 mg) Cells (20 million) | Tissue (4g) Cells (300 million) | Tissue (125 mg) Cells (125 mg) | Tissue (25 mg) Cells (25 million) |
| Number of buffers and reagents used | 5 | 7 | 3 | 2 |
| Buffer preparation prior to use | Required | Required | Required | Not Required |
| Homogenizer | Required | Required | Required | Not Required |
| Density gradient formation | Required | Required | Required | Not Required |
| Ultracentrifugation | Required | Required | Required | Not Required |
| Purity of Isolated Lysosomes | Enriched lysosomes | Enriched lysosome | Enriched lysosome | Enriched lysosome |
| Approximate Protocol Time | About 3h | 5-12h | About 3h | About 1.2h |
Comparison of Major Golgi Apparatus Isolation Kits
The Golgi apparatus is consisted of a series of flattened membrane vesicles. The major function of Golgi is to modify proteins for transportation. Modified proteins are transported out of Golgi in coated vesicles that bud off the trans‐cellular membrane. It plays a critical role in protein synthesis and modification. There are limited selections of commercial kits for isolation of this important organelle.
| Brand/manufacturer | Brand# 1 | Invent Biotech |
| Product Name | Golgi Isolation Kit | MinuteTM Golgi Apparatus Enrichment Kit |
| Mechanism of Isolation | Density gradient centrifugation | Spin column-based -precipitation |
| Average sample size | Tissue (5 g) | Tissue (35 mg) Cells (30 million) |
| Number of buffers and reagents used | 3 | 4 |
| Buffer preparation prior to use | Required | Not Required |
| Homogenizer | Required | Not Required |
| Density gradient formation | Required | Not Required |
| Ultracentrifugation | Required | Not Required |
| Purity of Isolated Golgi | Enriched Golgi | Enriched cis-Golgi and secretory vesicles of trans-Golgi |
| Approximate Protocol Time | 3.5h | 2h |
Comparison of Major ER Isolation Kits
The endoplasmic reticulum (ER) is a membranous labyrinth extending throughout the cell. The ER is a major organelle and accounts for about 1/2 of total cellular membrane. It plays a critical role in protein synthesis and modification. There are a few commercial kits available for ER isolation with different mechanisms of actions.
| Brand/manufacturer | Brand# 1 | Brand# 2 | Invent Biotech |
| Product Name | ER Enrichment Kit | Endoplasmic Reticulum Isolation Kit | MinuteTM ER Enrichment Kit |
| Mechanism of Isolation | Differential centrifugation and precipitation | Density gradient centrifugation | Spin column-based precipitation |
| Average sample size | Tissue (500 mg) | Tissue (3-7 g) Cells (2000 million) | Tissue (35 mg) Cells (30 million) |
| Number of buffers and reagents used | 5 | 4 | 4 |
| Buffer preparation prior to use | Required | Required | Not Required |
| Homogenizer | Required | Required | Not Required |
| Density gradient formation | Not Required | Required | Not Required |
| Ultracentrifugation | Not Required | Required | Not Required |
| Purity of Isolated ER | Enriched ER | Enriched ER | Enriched ER |
| Approximate Protocol Time | 1-2h | 1.5-5h | 1.5h |
Comparision of Major Commercial Plant Nuclei Isolation Kits
The nuclear proteome has recently gained importance in basic and applied research due to the fact that identification of novel nuclear proteins helps to understand protein functions and protein-protein/protein-nucleic acid interactions. Currently there just a few commercial kits available for isolation of plant nuclei. Following table is a side by side comparison of two major brands.
| Brand/Manufacturer | Leading Brand | MinuteTM (Invent Biotechnologies, Inc.) |
| Product Name | Plant Nuclei Isolation /Extraction Kit | MinuteTM Plant Cytosolic and Nuclear Protein Isolation Kit |
| Mechanism of Isolation | Solution-based | Spin column-based |
| Average sample size | 20 g | 150 mg |
| List of required buffers | Nuclei Isolation Buffer 4X (NIB) Percoll Sucrose 2.3 M TRITONX-100 10% Extraction Buffer Nuclei PURE Storage Buffer | Buffer A Buffer B Buffer C |
| Liquid nitrogen | Required | Not required |
| Buffer dilution prior to experiment | Required | Not required |
| Fractions obtained | Nuclei | Cytosol Microsomal Chloroplast Nuclei |
| Purity of isolated Nuclei | Low to high | High |
| Protocol Time | >90 min | <60 min |
Your first choice for scientific solutions
Find out more
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations