Invent Biotechnologies Comparisions

Comparison of Major Lipid Raft Isolation Kits

Lipid rafts are important subcellular structures rich in cholesterol and sphingolipid and signaling proteins. Lipid rafts play a critical role in cellular transport and signal transduction. Traditional method of lipid raft isolation is tedious and time consuming. There are limited numbers of commercial kits for isolation of this important structure.

Brand/manufacturerBrand# 1Invent Biotech
Product NameCaveolae/Rafts Isolation KitMinuteTM Total Lipid Raft Isolation Kit for Mammalian Cells/Tissues
Mechanism of IsolationUltracentrifugation flotation Spin column-based - floatation with a table top centrifuge
Average sample sizeCells (20-50 million) Tissue (35 mg) Cells (20-30 million)
Number of buffers and reagents used63
Buffer preparation prior to use RequiredNot Required
Density gradient formationRequiredNot Required
UltracentrifugationRequiredNot Required
Purity of Isolated Lipid RaftsTotal Detergent Resistant lipid RaftsTotal Detergent Resistant lipid Rafts
Approximate Protocol Time>5 h1 h

Comparison of Major Lysosome Isolation Kits

Lysosomes are membranous vesicles that are rich in hydrolytic enzymes capable of breaking down proteins, nuclei acids, carbohydrates and other cellular components. There are several commercial kits available for lysosome isolation. However, by mechanism of isolation, there are only two types of kits: traditional density gradient centrifugation-based and novel spin column-based.

Brand/manufacturerBrand# 1Brand# 2 Brand# 3 Invent Biotech
Product NameLysosome Isolation Kit from Tissue and Cultured Cells Lysosome Isolation Kit Lysosome Enrichment Kit for Tissue and Cultured cells MinuteTM Lysosome Isolation Kit
Mechanism of IsolationDensity gradient centrifugation Density gradient centrifugation Density gradient centrifugation Spin column-based precipitation
Average sample sizeTissue (100 mg) Cells (20 million) Tissue (4g) Cells (300 million) Tissue (125 mg) Cells (125 mg) Tissue (25 mg) Cells (25 million)
Number of buffers and reagents used5732
Buffer preparation prior to use RequiredRequiredRequiredNot Required
HomogenizerRequiredRequiredRequiredNot Required
Density gradient formationRequiredRequiredRequiredNot Required
UltracentrifugationRequiredRequiredRequiredNot Required
Purity of Isolated Lysosomes Enriched lysosomesEnriched lysosomeEnriched lysosomeEnriched lysosome
Approximate Protocol TimeAbout 3h5-12hAbout 3hAbout 1.2h

Comparison of Major Golgi Apparatus Isolation Kits

The Golgi apparatus is consisted of a series of flattened membrane vesicles. The major function of Golgi is to modify proteins for transportation. Modified proteins are transported out of Golgi in coated vesicles that bud off the trans‐cellular membrane. It plays a critical role in protein synthesis and modification. There are limited selections of commercial kits for isolation of this important organelle.

Brand/manufacturerBrand# 1Invent Biotech
Product NameGolgi Isolation Kit MinuteTM  Golgi Apparatus  Enrichment Kit 
Mechanism of IsolationDensity gradient centrifugationSpin column-based -precipitation
Average sample sizeTissue (5 g)Tissue (35 mg) Cells (30 million)
Number of buffers and reagents used34
Buffer preparation prior to use RequiredNot Required
HomogenizerRequiredNot Required
Density gradient formationRequiredNot Required
UltracentrifugationRequiredNot Required
Purity of Isolated GolgiEnriched GolgiEnriched cis-Golgi and secretory vesicles of trans-Golgi
Approximate Protocol Time3.5h2h

Comparison of Major ER Isolation Kits

The endoplasmic reticulum (ER) is a membranous labyrinth extending throughout the cell. The ER is a major organelle and accounts for about 1/2 of total cellular membrane. It plays a critical role in protein synthesis and modification. There are a few commercial kits available for ER isolation with different mechanisms of actions.

Brand/manufacturerBrand# 1Brand# 2Invent Biotech
Product NameER Enrichment KitEndoplasmic Reticulum Isolation Kit MinuteTM ER Enrichment Kit
Mechanism of IsolationDifferential centrifugation and precipitation Density gradient centrifugationSpin column-based precipitation
Average sample sizeTissue (500 mg)Tissue (3-7 g) Cells (2000 million) Tissue (35 mg) Cells (30 million)
Number of buffers and reagents used544
Buffer preparation prior to use RequiredRequiredNot Required
HomogenizerRequiredRequiredNot Required
Density gradient formationNot RequiredRequiredNot Required
UltracentrifugationNot RequiredRequiredNot Required
Purity of Isolated ER Enriched EREnriched EREnriched ER
Approximate Protocol Time1-2h1.5-5h1.5h

Comparision of Major Commercial Plant Nuclei Isolation Kits

The nuclear proteome has recently gained importance in basic and applied research due to the fact that identification of novel nuclear proteins helps to understand protein functions and protein-protein/protein-nucleic acid interactions. Currently there just a few commercial kits available for isolation of plant nuclei. Following table is a side by side comparison of two major brands.

Brand/ManufacturerLeading BrandMinuteTM (Invent Biotechnologies, Inc.)
Product Name Plant Nuclei Isolation /Extraction KitMinuteTM Plant Cytosolic and Nuclear Protein Isolation Kit
Mechanism of IsolationSolution-basedSpin column-based
Average sample size20 g150 mg
List of required buffersNuclei Isolation Buffer 4X (NIB) Percoll Sucrose 2.3 M TRITONX-100 10% Extraction Buffer Nuclei PURE Storage BufferBuffer A Buffer B Buffer C
Liquid nitrogenRequiredNot required
Buffer dilution prior to experimentRequiredNot required
Fractions obtainedNucleiCytosol Microsomal Chloroplast Nuclei
Purity of isolated NucleiLow to highHigh
Protocol Time>90 min<60 min
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