LEXSY (Leishmania Expression System) is a eukaryotic protein expression platform based on the S1-classified protozoan L. tarentolae. It combines the advantages of prokaryotic systems (simple handling, fast growth) with those of eukaryotic ones (efficient folding, post-translational modifications).
More than 20 crystal structures of proteins produced with LEXSY have been reported so far, among them human Cu/Zn Superoxide dismutase, Channelrhodopsin 2 or Legumain.
Here LEXSY has been successfully employed by Ebner et al. to express an active dimeric chitinase from the gastrointestinal nematode Trichuris suis (Pig whipworm), which was suitable for in vitro and in vivo assays as well as for crystallization (Fig. 1).