LAMP – loop-mediated DNA amplification at constant temperature (Isothermal Amplification) offers key advantages over conventional PCR. Not only cost efficiency and simplicity (no thermocycler required!) are arguments for strand displacement techniques. Also a high level of tolerance towards a large range of PCR inhibitors that enables impure sample amplification and direct detection of target genes makes this future technology interesting for diagnostics, e.g. point-of-care applications worldwide.
Here we introduce a new innovative enzyme to reduce typical amplification times by a factor of 2 – 3. Saphir Bst2.0 Turbo Polymerase benefits from an additional DNA-binding domain to ensure ultra-fast and robust amplification at 60 – 65 °C. Detect your target gene in just 5 – 10 min!
