Cancer and Proliferation Marker Nucleosides
Selected nucleosides for measurement of cancer marker and detection of newly synthesized DNA and RNA.
Marker nucleosides such as 1-Methyl-adenosine, 5-Methyl-uridine, N3-Methyl-uridine and Pseudouridine are found to be
elevated in assocation with cancer[1-3]. Jena Bioscience offers proper nucleoside standards for their measurement.
Detection of de novo DNA synthesis or S-Phase synthesis is perfomed with modified nucleosides which are efficiently incorporated into proliferating cells and labeled subsequently. Br-dU can be tagged with the corresponding antibody [4-6]. 5-EdU will make the newly synthesized DNA visible when cells are incubated subsequently with a fluorescent azide, which is rapidly “click”-coupled to the alkyne group of 5-EdU. In contrast to Br-dU, the method does not require sample fixation or DNA denaturation and permits good structural preservation [7,8]. Analog 5-Ethynyl-2′-deoxy-cytidine can be used to detect DNA synthesis with similar sensitivity as EdU .
Based on the same principle, 5-EU can be used for detection of RNA transcription and turnover in vivo. As it is incorporated into RNA transcripts, 5-EU-labeled cellular RNA can be detected quickly and with high sensitivity with fluorescent azides .