Nucleosides in Cancer and Proliferation Marker Nucleosides

Cancer and Proliferation Marker Nucleosides

Selected nucleosides for measurement of cancer marker and detection of newly synthesized DNA and RNA.

Marker nucleosides such as 1-Methyl-adenosine, 5-Methyl-uridine, N3-Methyl-uridine and Pseudouridine are found to be
elevated in assocation with cancer[1-3]. Jena Bioscience offers proper nucleoside standards for their measurement.

Detection of de novo DNA synthesis or S-Phase synthesis is perfomed with modified nucleosides which are efficiently incorporated into proliferating cells and labeled subsequently. Br-dU can be tagged with the corresponding antibody [4-6]5-EdU will make the newly synthesized DNA visible when cells are incubated subsequently with a fluorescent azide, which is rapidly “click”-coupled to the alkyne group of 5-EdU. In contrast to Br-dU, the method does not require sample fixation or DNA denaturation and permits good structural preservation [7,8]. Analog 5-Ethynyl-2′-deoxy-cytidine can be used to detect DNA synthesis with similar sensitivity as EdU [10].

Based on the same principle, 5-EU can be used for detection of RNA transcription and turnover in vivo. As it is incorporated into RNA transcripts, 5-EU-labeled cellular RNA can be detected quickly and with high sensitivity with fluorescent azides [9].

Name Cat. No. Size
5-Ethynyl-cytidine (5-EC) CLK-087 10 mg
5-Ethynyl-2′-deoxycytidine (5-EdC) CLK-N003-10 10 mg
5-Ethynyl-2′-deoxyuridine (5-EdU) CLK-N001-25 25 mg
5-Ethynyl-2′-deoxyuridine (5-EdU) CLK-N001-100 100 mg
5-Ethynyl-2′-deoxyuridine (5-EdU) CLK-N001-100 500 mg
5-Ethynyl-2′-deoxyuridine (5-EdU) CLK-N001-5000 5 g
5-Ethynyl-uridine (5-EU) CLK-N002-10 10 mg

Selected References

[1] Seidel et al. (2006) Modified nucleosides: an accurate tumour marker for clinical diagnosis of cancer, early detection and therapy control. Br J Cancer 94:1726.
[2] Bullinger et al. (2007) Metabolic signature of breast cancer cell line MCF-7: profiling of modified nucleosides via LC-IT MS coupling. BMC Biochem 29:25.
[3] Kim et al. (2001) Capillary electrophoretic profiling and pattern recognition analysis of urinary nucleosides from uterine myoma and cervical cancer patients. Journal of Chromatography B: Biomedical Sciences and Applications 754:97.
[4] Gratzner et al. (1992) Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science 218:474.
[5] Qin et al. (1993) Comparison of the classical autoradiographic and the immunohistochemical methods with BrdU for measuring proliferation parameters in colon cancer. Anticancer Res 13:731.
[6] Morstyn et al. (1986) Immunohistochemical identification of proliferating cells in organ culture using bromodeoxyuridine and a monoclonal antibody. J Histochem Cytochem 34(6):697.
[7] Salic et al. (2008) A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc. Natl. Acad. Sci. USA 105 (7):2415.
[8] Buck et al. (2008) Detection of S-phase cell cycle progression using 5-ethynyl-2′-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2′-deoxyuridine antibodies. Biotechniques 44 (7 :927.
[9] Jao et al. (2009) Exploring RNA transcription and turnover in vivo by using click chemistry. Proc. Nat. Acad. Sci. USA 105 (41):15779.
[10] Qu et al. (2011) 5-Ethynyl-2′-deoxycytidine as a new agent for DNA labeling: detection of proliferating cells. Anal Biochem 417(1 :112.


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