The authors of A publication utilized the mouse PAI-1 total antigen assay ELISA kit from Molecular Innovations.
“PAI‐1 is a stress‐responsive gene that is highly induced in nonalcoholic steatohepatitis (NASH). Endoplasmic reticulum (ER) stress is a salient feature of NASH, yet it is unknown whether ER stress contributes to hepatic PAI‐1 induction in this disorder. Hepatic PAI-1 expression was measured in mice subjected to pharmacologic ER stress.”
PAI-1 antigen levels in mouse plasma and liver tissue homogenates were determined after treatment with tunicamycin to induce prolonged low‐grade ER stress. Hepatic and plasma PAI-1 concentrations were increased 72 hours after induction of ER stress.