In light of the impact from COVID-19, we have seen an increasing demand for reagents essential to lateral flow assays and point-of-care test development from our customers. Rapid diagnosis is a critical tool for screening at-risk populations and managing the spread of the outbreak. Development of sensitive lateral flow assays requires high performance, robust gold particles, and our BioReady gold reagents are a key component for maximizing the sensitivity and reliability of visual detection point of care tests.
Given the growing demand, we would like to support our customers in efforts to address the global community’s urgent need for rapid point-of-care testing.
nanocomposix covid-19
Research Solutions for Coronavirus – Nanocomposix
Coronavirus Disease 2019 (COVID-19) Response
New to covalent conjugation? Need increased assay sensitivity?
Imagine an optimized system with time-after-time consistency that provides increased sensitivity and saves you time and money!
The High Sensitivity Conjugation Kit is a complete “classroom-in-a-box” with detailed protocols to help you produce robust covalently-bound conjugates. This kit is available with our ultra-bright 150 nm gold nanoshells as the reporter particle and includes everything you need to optimize your conjugations.
Benefits of the kit include:
- Pre-validated consumables eliminate the risk of purchasing non-compatible reagents, saving weeks to months of unnecessary troubleshooting
- Increase your assay sensitivity up to 20-fold with 150 nm gold nanoshells compared to 40 nm gold* nanoparticles
- Reduces antibody costs up to 65% compared to physisorption
- Saves time by reducing the number of conjugations necessary to find optimal antibody pairs
Already a conjugation expert? This kit eliminates the need to pre-screen and validate critical reagents and supplies to ensure your best chance of success when starting out with covalent conjugation to gold nanoshells.
Kit includes 10 mL of BioReady 150 nm Gold Nanoshells with a carboxyl surface, provided at 20 OD. Contact us to order the kit with BioReady 40 or 80 nm Gold Nanospheres.
TEM Diameter: 155 nm ± 15 nm
Size Distribution (CV): ≤ 15%
Note: approximate ranges below are dependent on diameter
- Hydrodynamic diameter (DLS): TEM diameter plus 5 to 25 nm
- Zeta potential: –40 to –70 mV
- pH of solution: 6.0 to 9.5
- Particle surface: carboxyl
- Solvent: 0.02 mM potassium carbonate solution
- Peak wavelength (λmax): 770 to 840 nm
nanoComposix Gold Conjugation Kit offers the advantage of covalent binding in two different gold sizes – 40 nm and 80 nm – to quickly screen and optimize gold conjugates for robust, high-performing lateral flow assays.
This kit eliminates the need to pre-screen and validate critical reagents and supplies to ensure your best chance of success. Kit components include:
- BioReady carboxyl 40 nm or 80 nm gold particles, 20 OD
- Desalting column for removal of interfering reagents (NaCl, azide, tris, glycine)
- EDC and Sulfo-NHS
- All buffers necessary to complete the conjugation
- Step-by-step conjugation procedure and optimization
No experience needed! Our kit is the optimal solution for conjugation experts and anyone new to lateral flow looking to improve existing tests or develop the next novel LF assays.
| TEM Diameter | Size Distribution (CV) |
| 80 nm ± 5 nm | ≤ 15% |
| 40 nm ± 4 nm | ≤ 15% |
Note: approximate ranges below are dependent on diameter
- Hydrodynamic diameter (DLS): TEM diameter plus 0 to 30 nm
- Zeta potential: –25 to –80 mV
- pH of solution: 5.5 to 7.0
- Particle surface: PEG12-carboxylic acid
- Solvent: Milli-Q water
- Peak wavelength (λmax): 525 to 545 nm
Streptavidin tetramer is covalently bound to the particle surface via an amide bond. 40 nm streptavidin gold conjugates can be used for convenient conjugation of any biotinylated antibody, protein or oligonucleotide.
BioReady products are specially formulated for the attachment of a molecular recognition element such as an antibody for lateral flow and biodiagnostics.
Provided at 10 OD in an aqueous conjugation buffer composed of 1× PBS, 0.5% BSA, and 0.05% azide.
PROTOCOLS
Specifications & CoA
| TEM Diameter | Size Distribution (CV) | Example Certificate of Analysis |
|---|---|---|
| 40 nm ± 4 nm | ≤ 15% | Download Example CoA |
Note: approximate ranges below are dependent on diameter
- Hydrodynamic diameter (DLS): 50 to 70 nm
- Zeta potential: < –10 mV
- pH of solution: 6.8 to 8.2
- Particle surface: streptavidin
- Solvent: 1× PBS, 0.5% BSA, and 0.05% azide
- Peak wavelength (λmax): 520 to 530 nm
Citrate is the most common stabilizer used for passive conjugation to gold. The “bare” particle surface is stabilized with weakly-associated citrate molecules that can be easily displaced for passive conjguation.
BioReady products are specially formulated for the attachment of a molecular recognition element such as an antibody for lateral flow and biodiagnostics.
Provided at 20 OD in aqueous 0.02 mM sodium citrate.
PROTOCOLS
Specifications & CoA
| TEM Diameter | Size Distribution (CV) | Example Certificate of Analysis |
|---|---|---|
| 40 nm ± 4 nm | ≤ 15% | Download Example CoA |
| 80 nm ± 5 nm | ≤ 15% | Download Example CoA |
Note: approximate ranges below are dependent on diameter
- Hydrodynamic diameter (DLS): TEM diameter plus 0 to 10 nm
- Zeta potential: –25 to –80 mV
- pH of solution: 5.5 to 8.0
- Particle surface: citrate
- Solvent: aqueous 0.02 mM sodium citrate
- Peak wavelength (λmax): 520 to 530 nm
BioReady Passive Conjugation Kit
Use the BioReady Passive Conjugation Kit to conjugate any proteins to 40 nm and 80 nm bare citrate OD 20 gold particles.
Kit components:
- BioReady 40 nm bare citrate gold, OD 20, 5 mL
- BioReady 80 nm bare citrate gold, OD 20, 5 mL
- Titration buffers covering pH 6.0–9.7, 1 mL each
- Blocking buffer, 5 mL
- Conjugate diluent, 5 mL
- 10% NaCl for salt test, 5 mL
Our conjugation kit provides the flexibility to conjugate at any OD from 1 to 20. See Protocol below for more information.
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