Secondary Antibodies

Secondary antibodies to help with the detection of primary antibodies.

Our secondary antibodies include CHROMEOsity anti-rabbit & anti-mouse secondaries for enhanced fluorescent detection. These antibodies have unrivaled fluorescent intensity, low background and limited photobleaching. Additionally, we offer EZ-HRP™ Polymer Detection Antibodies which enhance avidin-biotin detection when compared with conventional secondary antibodies.

U2OS cells stained with propidium iodide were incubated with rabbit anti-tubulin antibody. Chromeo 488 Goat anti-Rabbit IgG secondary antibody was then used at 1:1000

Name Catalog # Type Species Applications Size
488 Goat-anti-Mouse IgG GT26000 Goat IgG Mouse IHC, IF 1 mg
546 Goat-anti-Mouse IgG GT26003 Goat IgG Mouse IHC, IF 1 mg
546 Goat-anti-Rabbit IgG GT26004 Goat IgG Rabbit IHC, IF 1 mg
642 Goat-anti-Mouse IgG GT26005 Goat IgG Mouse IHC, IF 1 mg
642 Goat-anti-Rabbit IgG GT26006 Goat IgG Rabbit IHC, IF 1 mg
Alkaline Phosphatase Goat Anti-Chicken IgY CH23102 Goat IgG Chicken WB, IHC, IF 1000 ul
Biotinylated Goat anti-Chicken IgY CH23103 Goat IgG Chicken IHC, FC, IF 500 ul
Fluorescein Goat Anti-Chicken IgY CH23101 Goat IgG Chicken IHC, IF 500 ul
Goat anti-guinea pig IgG (H+L), FITC Labeled GP40100 Guinea Pig IgG Guinea Pig IHC, IF 1.0 ml
Hen Blocker CH23106 Blocking Reagent Chicken ICC, WB, IHC, E, IF 100 ml
Horse anti-goat IgG, Biotin HO30002 Goat IgG Rabbit IHC 750 ug
Horseradish Peroxidase Goat Anti-Chicken IgY CH23104 Chicken IgY Chicken WB, IHC, IF 400 ul

Immunohistochemistry (IHC) is a state of the art technique which requires not only high-affinity primary antibodies but a robust and sensitive detection chemistry allowing to visualize low abundant protein targets. Conventional Avidin-Biotin detection is time-consuming, has a mediocre sensitivity and often results in producing a non-specific background. To overcome these drawbacks, we utilized a proprietary 3D polymerization technology and developed EZ-HRP™ Polymer Detection. This new conjugation strategy does not affect the affinity of antibodies and improves their penetration capacity into tissue sections.

Name Catalog # Species Applications Size
Anti-Mouse EZ-HRP™ Polymer Detection SF40007 Mouse IHC 15 ml
Anti-Rabbit EZ-HRP™ Polymer Detection SF40006 Rabbit IHC 15 ml
Anti-Rabbit/Mouse EZ-HRP™ Polymer Detection SF40008 Mouse, Rabbit IHC 15 ml

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