25% OFF ALL ANNEXIN V PRODUCTS

Promo code: ANXV25

Valid until December 31st 2019

See below the list of conjugates and assay kits included in this offer. Take a moment to read about the additional resources to guide you for the selection of the right conjugate.

Annexin V is a 35 kDa phospholipid-binding protein. Its strong calcium-dependent affinity for phosphatidylserine (PS) can be used to identify cells undergoing apoptosis. In viable cells, negatively charged PS residues are located on the cytosolic surface of the plasma membrane. However, during early apoptosis, cells loose plasma membrane asymmetry and PS residues translocate to the outer leaflet of the membrane, where they can be detected by fluorescently labelled annexin V.

Early-stage apoptosis detection with annexin V conjugates

Annexin V conjugated to iFluor™ dyes provide a fast and reliable method for detecting externalized PS, a key characteristic in early-stage apoptosis. The superior brightness and photostability of Annexin V iFluor™ probes significantly out-performs most Alexa Fluor® and other spectrally similar annexin v conjugates. Annexin V iFluor™ conjugates are useful for live cell imaging, immunofluorescence, and flow cytometry, and can be combined with other dyes, such as nucleic acid stains, to accurately assess mixed populations of apoptotic, necrotic and dead cells.

AAT Bioquest offers a wide variety of fluorescently labelled annexin V conjugates in colours ranging from UV to infrared for improved multiplexing flexibility. We also offer recombinant annexin V and easy-to-use kits optimized for flow cytometry, as well as, biotinylated annexin V, which can be detected using fluorophore-labelled streptavidin.

ADDITIONAL RESOURCES

Selection guide for Annexin V iFluor™ conjugates

Annexin V Conjugates for Flow Cytometry

Annexin V conjugated to mFluor™ dyes, PacBlue, APC and PE are valuable tools for measuring apoptosis by flow cytometry. These conjugates can be combined with other dyes, such as nucleic acid stains, to accurately assess mixed populations of apoptotic, necrotic and dead cells. We offer annexin V conjugates for flow cytomtery as stand-alone reagents or easy-to-use kits.

The benefits of our annexin V mFluor™ conjugates include:

• Superior brightness and improved photostability for long-term cellular imaging
• Large Stokes shifts to reduce cross-talk between the excitation source and fluorescence emission for cellular imaging with high signal-to-noise ratios
• Conjugates efficiently excited by common lasers for increased multiplexing capabilities

Selection guide for Annexin V mFluor™ conjugates optimized for flow cytometry

Cell Meter™ Annexin V Binding Apoptosis Assays

Cell Meter™ Annexin V Binding Apoptosis Assay Kits provide a fast and convenient method for measuring apoptotic cells based on the externalization of phosphatidylserine. Each kit contains a recombinant annexin V conjugated to either an iFluor™ dye, mFluor™ dye, APC, PE or FITC and used to detect translocated PS in cells undergoing early apoptosis. In addition, these kits (excluding 22825, 22826 and 22827) include a ready-to-use solution of propidium iodide (PI) or Nuclear Red™ DCS1 nucleic acid stain. Both PI and Nuclear Red™ DCS1 are impermeant to live cells but stain necrotic and dead cells with intense red fluorescence. After staining cells with annexin V conjugate and nucleic acid stain, apoptotic cells show annexin V conjugate fluorescence, dead cells show red and annexin V conjugate fluorescence, and live cells show little or no fluorescence. Cell populations can readily be differentiated using a flow cytometer.

Benefits of Cell Meter™ Annexin V Binding Apoptosis Assays include:

• High Sensitivity – annexin V conjugated to iFluor™ dyes and mFluor™ for brighter and more photostable signals
• Flexibility – annexin V conjugates for many lasers and detection channels
• Simplicity – assay procedure typically completed in under an hour

Figure 1. Cell Meter Annexin V Binding Assay Cat No. 22824. Jurkat cells were treated with 20 µM camptothecin (Red) in a 37 °C, 5% CO2 incubator for 4-5 hours or untreated (Blue). Cells were then treated with Annexin V iFluor™ 488 for 30 minutes to identify apoptotic cells and with propidium iodide to identify dead cells, followed by flow cytometric analysis using the FL1 channel.

Selection guide for Cell Meter™ Annexin V Binding Apoptosis Assay kits