25% OFF ALL ANNEXIN V PRODUCTS

AAT Bioquest Annexin V conjugates and assay kits offer

Promo code: ANXV25

Valid until December 31st 2019

See below the list of conjugates and assay kits included in this offer. Take a moment to read about the additional resources to guide you for the selection of the right conjugate.

Annexin-V Staining

 25% OFF ALL ANNEXIN V PRODUCTS

Annexin V is a 35 kDa phospholipid-binding protein. Its strong calcium-dependent affinity for phosphatidylserine (PS) can be used to identify cells undergoing apoptosis. In viable cells, negatively charged PS residues are located on the cytosolic surface of the plasma membrane. However, during early apoptosis, cells loose plasma membrane asymmetry and PS residues translocate to the outer leaflet of the membrane, where they can be detected by fluorescently labelled annexin V.

Early-stage apoptosis detection with annexin V conjugates

Annexin V conjugated to iFluor™ dyes provide a fast and reliable method for detecting externalized PS, a key characteristic in early-stage apoptosis. The superior brightness and photostability of Annexin V iFluor™ probes significantly out-performs most Alexa Fluor® and other spectrally similar annexin v conjugates. Annexin V iFluor™ conjugates are useful for live cell imaging, immunofluorescence, and flow cytometry, and can be combined with other dyes, such as nucleic acid stains, to accurately assess mixed populations of apoptotic, necrotic and dead cells.

AAT Bioquest offers a wide variety of fluorescently labelled annexin V conjugates in colours ranging from UV to infrared for improved multiplexing flexibility. We also offer recombinant annexin V and easy-to-use kits optimized for flow cytometry, as well as, biotinylated annexin V, which can be detected using fluorophore-labelled streptavidin.

Annexin V Product Ordering Information

Table1. Ordering information for annexin V (recombinant) and annexin V conjugates

Cat #Product NameEx (nm)Ex (nm)Size
20070Annexin V-iFluor™ 350 conjugate347443100 tests
20071Annexin V-iFluor™ 488 conjugate494520100 tests
20072Annexin V-iFluor™ 555 conjugate552567100 tests
20073Annexin V-iFluor™ 594 conjugate590615100 tests
20069Annexin V-iFluor™ 633 conjugate638655100 tests
20074Annexin V-iFluor™ 647 conjugate651660100 tests
20075Annexin V-iFluor™ 680 conjugate681705100 tests
20077Annexin V-iFluor™ 700 conjugate690716100 tests
20076Annexin V-iFluor™ 750 conjugate750778100 tests
20080Annexin V-mFluor™ Violet 450 conjugate403454100 tests
20081Annexin V-mFluor™ Violet 510 conjugate414510100 tests
20082Annexin V-mFluor™ Violet 540 conjugate424560100 tests
20085Annexin V-mFluor™ Blue 570 conjugate505570100 tests
20090Annexin V, AF350 conjugate346445100 tests
20092Annexin V, AF488 conjugate494517100 tests
20096Annexin V, AF594 conjugate590617100 tests
20030Annexin V, FITC Labeled494519100 tests
20089Annexin V, PacBlue conjugate410455100 tests
20031Annexin V, TRITC Labeled541568100 tests
20065Annexin V-Cy3 conjugate556574100 tests
20066Annexin V-Cy5 conjugate651660100 tests
20067Annexin V-Cy5.5 conjugate681705100 tests
20068Annexin V-Cy7 conjugate750778100 tests
20018Annexin V-Biotin conjugate100 tests
20014Annexin V, Recombinant100 ug
20015Annexin V, Recombinant1 mg

Table 2. Ordering information for annexin V binding apoptosis assay kits

Cat #Product NameEx (nm)Ex (nm)Size
22828Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Blue Fluorescence Excited at 405 nm*405450100 tests
22827Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*650668100 tests
22829Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Excited at 405 nm*414508100 tests
22824Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Green Fluorescence Optimized for Flow Cytometry*490520100 tests
22830Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Excited at 405 nm*424560100 tests
22825Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*554578100 tests
22826Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Red Fluorescence Optimized for Flow Cytometry*590610100 tests
22837Cell Meter™ APC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*651662100 tests
22839Cell Meter™ FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*490520100 tests
22838Cell Meter™ PE-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*565575100 tests

ADDITIONAL RESOURCES

Selection guide for Annexin V iFluor™ conjugates

 25% OFF ALL ANNEXIN V PRODUCTS

Annexin V Conjugates for Flow Cytometry

Annexin V conjugated to mFluor™ dyes, PacBlue, APC and PE are valuable tools for measuring apoptosis by flow cytometry. These conjugates can be combined with other dyes, such as nucleic acid stains, to accurately assess mixed populations of apoptotic, necrotic and dead cells. We offer annexin V conjugates for flow cytomtery as stand-alone reagents or easy-to-use kits.

The benefits of our annexin V mFluor™ conjugates include:

  • Superior brightness and improved photostability for long-term cellular imaging
  • Large Stokes shifts to reduce cross-talk between the excitation source and fluorescence emission for cellular imaging with high signal-to-noise ratios
  • Conjugates efficiently excited by common lasers for increased multiplexing capabilities

Selection guide for Annexin V mFluor™ conjugates optimized for flow cytometry

 25% OFF ALL ANNEXIN V PRODUCTS

Cell Meter™ Annexin V Binding Apoptosis Assays

Cell Meter™ Annexin V Binding Apoptosis Assay Kits provide a fast and convenient method for measuring apoptotic cells based on the externalization of phosphatidylserine. Each kit contains a recombinant annexin V conjugated to either an iFluor™ dye, mFluor™ dye, APC, PE or FITC and used to detect translocated PS in cells undergoing early apoptosis. In addition, these kits (excluding 22825, 22826 and 22827) include a ready-to-use solution of propidium iodide (PI) or Nuclear Red™ DCS1 nucleic acid stain. Both PI and Nuclear Red™ DCS1 are impermeant to live cells but stain necrotic and dead cells with intense red fluorescence. After staining cells with annexin V conjugate and nucleic acid stain, apoptotic cells show annexin V conjugate fluorescence, dead cells show red and annexin V conjugate fluorescence, and live cells show little or no fluorescence. Cell populations can readily be differentiated using a flow cytometer.

Benefits of Cell Meter™ Annexin V Binding Apoptosis Assays include:

  • High Sensitivity – annexin V conjugated to iFluor™ dyes and mFluor™ for brighter and more photostable signals
  • Flexibility – annexin V conjugates for many lasers and detection channels
  • Simplicity – assay procedure typically completed in under an hour
 25% OFF ALL ANNEXIN V PRODUCTS

Figure 1. Cell Meter Annexin V Binding Assay Cat No. 22824. Jurkat cells were treated with 20 µM camptothecin (Red) in a 37 °C, 5% CO2 incubator for 4-5 hours or untreated (Blue). Cells were then treated with Annexin V iFluor™ 488 for 30 minutes to identify apoptotic cells and with propidium iodide to identify dead cells, followed by flow cytometric analysis using the FL1 channel.

Selection guide for Cell Meter™ Annexin V Binding Apoptosis Assay kits

 25% OFF ALL ANNEXIN V PRODUCTS

SUPPORT

outstanding technical support

PRODUCT

we offer a full product guarantee

DELIVERY

we offer free delivery to UK universities and non profit organisations