Coronavirus

What’s Available to Study Novel Coronavirus 2019-nCoV?

The ongoing pneumonia outbreak in the city of Wuhan is caused by a newly identified coronavirus 2019-nCoV, named by the World Health Organization on January 12nd, 2020.This new virus belongs to the Betacoronavirus Genus, which also includes SARS CoV (2003) and MERS CoV (2012). The sequence reveals that 2019-nCoV is more similar to SARS-CoV (70% similarity) than to MERS-CoV (40%).

As part of our comprehensive Infectious disease portfolio, Stratech can offer a range of novel coronavirus 2019-nCoV primary antibodies and antigens (details below), 2019-nCoV multiplex RT-qPCR detection kit and NGS library prep kit.

Same as all other coronaviruses, the genome of 2019-nCOV encodes the spike protein, the envelope protein, the membrane protein, and the nucleocapsid protein.

The spike protein (S-protein) mediates receptor binding and membrane fusion. Spike protein contains two subunits, S1 and S2. S1 contains a receptor binding domain (RBD), which is responsible for recognizing and binding with the cell surface receptor. S2 subunit is the “stem” of the structure, which contains other basic elements needed for the membrane fusion. The spike protein is the common target for neutralizing antibodies and vaccines.

Coronavirus
Coronavirus

It’s been reported that 2019-nCoV (ref.) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. Indeed, the recombinant Spike protein can bind with recombinant ACE2 protein.

Reference: Xintian Xu.Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission.SCIENCE CHINA Life Sciences.2020

The nucleocapsid Protein (N-protein) is the most abundant protein in coronavirus. The N-protein is a highly immunogenic phosphoprotein, and it is normally very conserved. The N protein of coronavirus is often used as a marker in diagnostic assays.

Below is a list of relevant tools for 2019-nCOV research, including cDNA clones, antigens, detection and sequencing kits which have been developed immediately after the sequence was published. The recombinant antigens include the full length S protein (ECD), the S1 subunit, the S2 subunit, the S1 RBD, and the N protein.

2019-nCoV Antigen cDNA / Gene - expression plasmid (Codon Optimized)

CatalogueNameRefSeqLength (bp)
VG40588-UT2019-nCoV Nucleoprotein / NP ORF mammalian expression plasmid 2019-nCoV1260
VG40589-UT2019-nCoV Spike ORF mammalian expression plasmid 2019-nCoV3822
VG40592-UT2019-nCoV Spike(RBD) ORF mammalian expression plasmid 2019-nCoV675
VG40590-UT2019-nCoV Spike(S2) ORF mammalian expression plasmid 2019-nCoV1770
VG40591-UT2019-nCoV Spike(S1) ORF mammalian expression plasmid 2019-nCoV2058

2019-nCoV Antigen Protein

CodeNameExpression Host
40589-V08B12019-nCoV Spike Protein (S1+S2 ECD, His tag)Baculovirus-Insect Cells
40591-V05H12019-nCoV Spike Protein (S1 Subunit, mFc Tag)Human Cells
40592-V05H2019-nCoV Spike Protein (RBD, mFc Tag)Human Cells
40588-V08B2019-nCoV Nucleocapsid Protein (His tag)Baculovirus-Insect Cells
40591-V08B12019-nCoV Spike Protein (S1 Subunit, His tag)Baculovirus-Insect Cells
40591-V08H2019-nCoV Spike Protein (S1 Subunit, His Tag)Human Cells
40590-V08B2019-nCoV Spike Protein (S2 ECD, His tag)Baculovirus-Insect Cells
40591-V02H2019-nCoV Spike Protein (S1 Subunit, Fc Tag)Human Cells

2019-nCoV RT-qPCR detection kit

CodeNameSize
CD201-022019-Novel Coronavirus (2019-nCoV) Multiplex RT-qPCR Detection Kit100 tests

Novel coronavirus 2019 NGS library prep kits

CodeNameSize
YS-NGS-nCoV19-IL-962019-nCoV NGS library prep kit for Illumina sequencing96 samples (Illumina)
YS-NGS-nCoV19-IT-962019-nCoV NGS library prep kit for Ion Torrent sequencing96 samples (Ion Torrent)

2019-nCoV is part of the same family as the SARS virus, which caused a major outbreak in 2003. This new virus shares 89% similar to SARS-related bat coronavirus.
As the nucleocapsid (N) protein is highly conserved across coronavirus families cross-reactivity between 2019-nCoV and SARS N proteins (90% homologous) should also allow already commercially available antibodies and antigens to SARS N protein to be used for diagnostic testing for Wuhan 2019-nCoV
Our range of primary antibodies and antigens to Coronavirus have been validated for use in applications from ELISA and Western Blot to Lateral flow and Immuno Precipitation. The SARS antigens and polyclonal antibodies listed below should also allow serological testing for the new 2019-nCov virus.

CodeNameCategoryApplicationsReactivity
orb433317Coronavirus antibodyMonoclonal antibodyELISA, FACS, ICC, IF, IHC-Fr, IHC-P, WBVirus
orb433926Coronavirus antibody (Biotin)Monoclonal antibodyELISAVirus
orb544520Coronavirus nucleocapsid antibodyMonoclonal antibodyELISA, IP, LF, Purification, WBCanine
Orb434186Coronavirus surface antigen antibodyMonoclonal antibodyELISAVirus
orb544521Coronavirus nucleocapsid antibodyMonoclonal antibodyELISA, IP, LF, Purification, WBCanine
orb24061Bovine Coronavirus antibodyMonoclonal antibodyELISA, HAIBovine
orb544480Coronavirus antibodyMonoclonal antibodyELISA, PurificationCanine
orb544481Coronavirus antibodyMonoclonal antibodyELISA, PurificationCanine
orb544482Coronavirus antibodyMonoclonal antibodyELISA, PurificationCanine
orb544483Coronavirus antibodyMonoclonal antibodyELISA, PurificationCanine
orb544522Coronavirus antibodyPolyclonal AntibodyELISA, IP, PurificationCanine
orb23300SARS-CoV, Nucl. (340-390) recombinant proteinPolyclonal antibodyELISAVirus
orb23284SARS-CoV, Nucl. (340-390) recombinant proteinPolyclonal antibodyELISAVirus
orb544474Canine Coronavirus proteinProteinELISACanine
orb82497SARS-CoV Spike recombinant proteinRecombinant ProteinELISA, WB-
orb82466SARS-CoV, Nucl. (1-49) recombinant proteinRecombinant ProteinELISA, WB-
orb82489SARS-CoV Spike proteinRecombinant ProteinELISA, WB-
orb82484SARS-CoV, Spike (Middle) recombinant proteinRecombinant ProteinELISA, WB-
orb82482SARS-CoV, E Protein, recombinant proteinRecombinant ProteinELISA, WB-
orb82478SARS-CoV, E Protein, recombinant proteinRecombinant ProteinELISA, WB-
orb82471SARS-CoV, Nucl. (340-390) recombinant proteinRecombinant ProteinELISA, WB-
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