NEW “CLICKable” Desthiobiotinylation Reagents From Jena Bioscience

Labeling of biomolecules with biotin is routinely performed for their subsequent affinity purification via streptavidin agarose or detection with fluorescent or HRP-labeled streptavidin. A limitation of the biotin/streptavidin interaction is its irreversibility under physiological conditions.

The single-ring/sulfur-free biotin analog Desthiobiotin however binds less tightly to streptavidin, allowing specific elution from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin[1].



Now available: CLICK-functionalized reagents for Desthiobiotinylation of Alkyne-, Azide- and DBCO-tagged biomolecules via Cu(I)-free or Cu(I)-catalyzed Click Chemistry.

CLICK Functionality Product
Alkyne Acetylene-PEG4-Desthiobiotin
Azide Azide-PEG3-Desthiobiotin
DBCO DBCO-PEG4-Desthiobiotin


Table 1: Available Desthiobiotinylation Reagents with extended PEG3- or PEG4 spacer for increased molecule solubility & optimized streptavidin binding.

[1] Hirsch et al. (2002) Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation. Anal. Biochem. 308:343.

CLICK Chemistry reagents are not in stock they may be available as custom synthesis, just contact with any questions you may have!

For more information on Jena Biosciece products visit there mini-site and product listing.


outstanding technical support


we offer a full product guarantee


we offer free delivery to UK universities and non profit organisations