NEW from System Biosciences (SBI) the phiC31 Integrase Cloning system for mammalian genome engineering applications. This system provides the researcher with the tools to perform non-viral, transfection-based experiments that result in
single-copy insertion of transgenes of interest with minimal risk of off-target effects, and can be easily adapted for in vivo applications.
One-step gene addition technology
The phiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage phiC31. The phiC31 integrase mediates recombination between two 34 base pair sequences termed attachment sites (att), one found in the phage and the other in the bacterial host. This serine integrase has been show to function efficiently in many different cell types including mammalian cells. In the presence of phiC31 integrase, an attB- containing donor plasmid can be unidirectional integrated into a target genome through recombination at sites with sequence similarity to the native attP site (termed pseudo-attP sites). phiC31 integrase can integrate a plasmid of any size, as a single copy, and requires no cofactors. The integrated transgenes are stably expressed and heritable.
|PhiC31 Integrase Expression Plasmid||10ug||FC200PA-1|
|pFC-MCS-pA-SV40-Neo PhiC31 Donor Vector||10 ug||FC500A-1|
|pFC-CMV-MCS-pA-SV40-Neo PhiC31 Donor Vector||10 ug||FC501A-1|
|pFC-CMV-GFP-SV40-Neo Positive Control Donor Vector||10 ug||FC520A-1|
|pFC-EF1-MCS-pA-PGK-RFP-T2A-Puro PhiC31 Donor Vector||10 ug||FC550A-1|
|pFC-PGK-MCS-pA-EF1-GFP-T2A-Puro PhiC31 Donor Vector||10 ug||FC551A-1|