phiC31 Integrase Vector System One-step Gene Addition Technology

NEW from System Biosciences (SBI) the phiC31 Integrase Cloning system for mammalian genome engineering applications. This system provides the researcher with the tools to perform non-viral, transfection-based experiments that result in
single-copy insertion of transgenes of interest with minimal risk of off-target effects, and can be easily adapted for in vivo applications. 

Benefits

• Single-copy, non-viral delivery system
• Simple and effective way to generate stable cell lines and to genetically modify animals
• Sustainable transgene expression (sometimes an issue for viral systems)
• Low risk of insertional mutagenesis (which can often lead to oncogenic phenotype or chromosomal
• abnormalities)
• Ideal for stable transgene delivery to primary cells (which are often much more sensitive to random viral
• integration effects)
• Optimal for translating to in vivo applications (including gene therapy and regenerative medicine).
• Single transfection protocol
• Works in humans, mice, rats, pigs, chickens, cows, flies, frogs, and yeast (i.e. most mammalian and
• eukaryotic model systems)
• Extensive publication record for use in vitro and in vivo

Nonviral transgene delivery

One-step gene addition technology

The phiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage phiC31. The phiC31 integrase mediates recombination between two 34 base pair sequences termed attachment sites (att), one found in the phage and the other in the bacterial host. This serine integrase has been show to function efficiently in many different cell types including mammalian cells. In the presence of phiC31 integrase, an attB- containing donor plasmid can be unidirectional integrated into a target genome through recombination at sites with sequence similarity to the native attP site (termed pseudo-attP sites). phiC31 integrase can integrate a plasmid of any size, as a single copy, and requires no cofactors. The integrated transgenes are stably expressed and heritable.

Features

• Nonviral transgenesis
• Single-copy integration
• Preferred integration at active transcription sites
• Unlimited cargo capacity
• Easily make stable cell lines using transfection-based methods
• Suitable for engineering Stem Cells and In vivo animal models