Alithea Genomics, high throughput transcriptomics solutions for research and biomarker discovery.
Once all the samples have been barcoded, they can be immediately pooled together in one tube, which drastically reduces costs and manual operations of the downstream steps and, as a consequence, of the overall workflow.
96, 384 RNA-seq library preps in one tube.
For more reactions, higher multiplexing and more UDIs
Perform up-to 384 RNA-seq library preparations in one single tube.
Demultiplex and align your BRB-seq data with our easy-to-use cloud-based platform.
For projects of all sizes:
from 96 to 36’864 reactions in one kit.
All-inclusive.
Convenient and short protocol from samples to sequencing-ready libraries in one day.
| For use with (application) | 3’ mRNA sequencing |
| For use with (equipment) | Illumina NGS instruments |
| Species compatibility | All eukaryotic species |
| Available formats | 96, 384, 1’536, 6’144 and 36’864 reactions |
| Shipping conditions | Dry ice |
| Storage conditions | -20C |
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.
One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.
As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.
Our BRB-seq and DRUG-seq services deliver raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.
96, 384 RNA-seq library preps in one tube.
For more reactions, higher multiplexing and more UDIs
More samples, more replicates. Robust results, significant discoveries.
Demultiplex and align your BRB-seq data with our easy-to-use cloud-based platform.
Seamlessly integrated globin depletion. No need to purchase additional kits.
Convenient and short protocol from samples to sequencing-ready libraries in one day.
| For use with (application) | 3’ mRNA sequencing |
| For use with (equipment) | Illumina NGS instruments |
| Species compatibility | All eukaryotic species |
| Available formats | 96, 384, 1’536, 6’144 and 36’864 reactions |
| Shipping conditions | Dry ice |
| Storage conditions | -20C |
Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The recommended range of RNA amount for each sample is of 50ng-1μg, normally the more RNA, the better.
The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.
As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.
Our BRB-seq and DRUG-seq services deliver raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.
RNA-seq library preps directly from cell lysates without prior RNA isolation.
For more reactions, higher multiplexing and more UDIs
More samples, more replicates. Robust results, significant discoveries.
Demultiplex and align your DRUG-seq data with our easy-to-use cloud-based platform.
An optimized lysis buffer for complete lysis and efficient reverse transcription.
Without pre-amplification, leading to higher mapping and gene detection rates.
Convenient and short protocol from samples to sequencing-ready libraries in one day.
| For use with (application) | 3’ mRNA sequencing |
| For use with (equipment) | Illumina NGS instruments |
| Species compatibility | All eukaryotic species |
| Available formats | 96, 384, 1’536, 6’144 and 36’864 reactions |
| Shipping conditions | Dry ice |
| Storage conditions | -20C |
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.
For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.
Our BRB-seq and DRUG-seq services deliver raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.
RNA-seq library preps directly from cell lysates without prior RNA isolation.
For more reactions, higher multiplexing and more UDIs
More samples, more replicates. Robust results, significant discoveries.
Demultiplex and align your DRUG-seq data with our easy-to-use cloud-based platform.
An optimized lysis buffer for complete lysis and efficient reverse transcription.
Without pre-amplification, leading to higher mapping and gene detection rates.
Convenient and short protocol from samples to sequencing-ready libraries in one day.
| For use with (application) | 3’ mRNA sequencing |
| For use with (equipment) | Illumina NGS instruments |
| Species compatibility | All eukaryotic species |
| Available formats | 96, 384, 1’536, 6’144 and 36’864 reactions |
| Shipping conditions | Dry ice |
| Storage conditions | -20C |
Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The only difference between BRB-seq and standard RNA-seq data analysis is the demultiplexing step, which is used to assign sequencing reads to their sample of origin based on the BRB-seq barcode sequence.
For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.
Our BRB-seq and DRUG-seq services deliver raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.
96, 384 RNA-seq library preps in one tube.
For more reactions, higher multiplexing and more UDIs
Specially designed for MGI systems, no additional library conversion kit is needed.
Perform up-to 96 RNA-seq library preparations in one single tube.
Demultiplex and align your BRB-seq data with our easy-to-use cloud-based platform.
Convenient and short protocol from samples to sequencing-ready libraries in one day.
| For use with (application) | 3’ mRNA sequencing |
| For use with (equipment) | MGI T and G series instruments |
| Species compatibility | All eukaryotic species |
| Available formats | 96, 384 reactions |
| Shipping conditions | Dry ice |
| Storage conditions | -20C |
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
The minimum recommended RIN number is 6 and the A260/230 ratio (Nanodrop) should be in the 1.5-2.2 range.
For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.
One of the key advantages of BRB-seq is that it does not only save reagents and cost in the library preparation stage, but also in the sequencing one.
As opposed to standard RNA-seq, where 20M-30M reads per sample are required, we normally recommend to sequence BRB-seq libraries at a depth of 4M-5M reads per sample, which is normally enough to detect the vast majority of expressed genes.
Our BRB-seq and DRUG-seq services deliver raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.
RNA-seq library preps directly from cell lysates without prior RNA isolation.
For more reactions, higher multiplexing and more UDIs
An optimized lysis buffer for complete lysis and efficient reverse transcription.
Without pre-amplification, leading to higher mapping and gene detection rates.
More samples, more replicates. Robust results, significant discoveries.
Specially designed for MGI systems, no additional library conversion kit is needed.
Demultiplex and align your DRUG-seq data with our easy-to-use cloud-based platform.
Convenient and short protocol from samples to sequencing-ready libraries in one day.
| For use with (application) | 3’ mRNA sequencing |
| For use with (equipment) | MGI T and G series instruments |
| Species compatibility | All eukaryotic species |
| Available formats | 96, 384 reactions |
| Shipping conditions | Dry ice |
| Storage conditions | -20C |
Each BRB-seq kit contains reagents (including four pairs of Unique Dual Indexing adapters) sufficient for the complete library preparation process for four different BRB-seq pools.
To note, the total number of RNA samples that can be processed with one kit does not exceed the kit specifications; for instance, a 96-samples kit can be used to prepare up-to 96 samples distributed across up-to four different libraries.
For a thorough description of BRB-seq data processing, please refer to the BRB-seq kit user guide. In order to facilate as much as possible BRB-seq data analysis, we have also created a dedicate pipeline which can be found in our “SOFTWARE” page.
Our BRB-seq and DRUG-seq services deliver raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.
Demultiplexing, alignment and gene counts at the click of a button.
Download results
Our BRB-seq and DRUG-seq kits make it possible to process up to 384 RNA samples in one single tube, which helps reduce technical variation, turnaround times and costs. Experience sample to sequencing-ready library in one day.
Our BRB-seq and DRUG-seq services deliver raw sequencing data (fastq files), gene count matrices and analysis report files. A cost-efficient option suitable for projects of all sizes.
By leveraging BRB-seq, we not only provide to industrial and academic clients high quality RNA-seq data, but we also do so with the highest affordability and shortest turnaround times on the market.
Massively multiplexed RNA-seq offers unparalleled cost and throughput scalability.
You can trust us. We have ran projects from as low as 6 samples to as many as 100’000.
From samples to data in as fast as 1.5 months.
The MERCURIUS™ BRB-seq service offers a convenient and streamlined solution for transcriptomics projects of any size. Clients can send us purified RNA samples, which are always quality-checked before launching our BRB-seq pipeline. During the process, we always keep clients informed at defined checkpoint so that we can decide together how to best proceed to the next steps.
Next generation sequencing and data pre-processing (including alignment to the genome of choice) are part of our standard service as well. As a result, we provide our clients raw data, sequencing and alignment reports, and gene count matrices which can be used for downstream gene expression analysis.
To guarantee high quality data, we normally request that each sample contains at least 200ng of total RNA in at least 10μl.
As part of our standard service pipeline, we align the generated data to the genome of choice, provide a detailed report on the alignment and gene counting statistics and, finally, provide ready-to-use gene count matrices for downstream analysis.
Optionally, we can include differential gene expression analysis.
