CH3 BioSystems Provides Our Customers With A Protected Array Of Unique Products, Standard And Customized Kits And Protocols Anchored By The Patented CH3 Antibody.


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CH3 Biosystems

Our flagship methylarginine-specific antibody, anti-mRG, is specifically designed to recognize the greatest number of methylproteins currently known. The immunizing antigen is based on a site of methylation found in a minimally required peptide sequence of consensus for arginine methylation.

This tool provides a fundamental advantage for research that is exploring the importance of protein methylation in biological systems.

Additional products now available for sale from our catalog and others in development are designed to promote simple and rapid advance in the biomedical analysis of protein methylation.

Our Mission

To provide our customers, the Basic Science Research and Clinical Laboratory communities, with world-class, high-performance products for the discovery of protein methylation pathways in cells and tissues.

To treat our customers as strategic business partners through superior research services to ensure success in the expansion of the field of protein methylation biology.

To broaden the biotechnology market through development of screening technologies to identify and commercialize drug and diagnostic candidates for the treatment of diseases such as cancer and neuro-developmental disorders.

CELL & GENE THERAPY
YOUR SOURCE FOR AUTHENTICATED CELL LINES

Who is CH3 BioSystems?

• CH3 Biosystems (CH3) was founded in 2007.
• Our first sales began in 2008.
• We now make direct sales worldwide, as well as through distribution channels in Europe, the UK, Japan, and Korea.
• Our main focus is biotechnology for cell and molecular research in protein methylation, but our unique authenticated, pathogen-tested clonal cell lines have become a valuable resource for general academic and corporate research, including bio-manufacturing.

Why choose our cell lines?

The provenance of each CH3 cell line is described in a “Certificate of Cell Line Identity”, which includes the original source of the cell
line, its acquisition date and the adaptation of the cell line to standardized production and testing practices developed by CH3 to deliver
optimum growth and long-term storage and viability.
Every production lot of each CH3 cell line is authenticated by short tandem repeat genetic profiling and pathogen testing prior to release
from inventory. Clients and customers are presented with an individualized, detailed report from our independent bioanalytical testing
laboratory.

Why license cells through CH3?

Cell and Gene Therapy (CGT) programs have the potential to revolutionize the treatment of devastating diseases. The first significant
bottleneck to developing potentially transformative CGT therapeutics for large-scale commercial production is the sourcing of authentic
cell lines. Before assuming the challenges of CGT development, it is important to secure the cells used for the bio-manufacturing process
from a reliable biotechnology partner with legitimate vested intellectual property and commercial rights to produce and maintain these
biological resources.

Figure 1: E0771 Cells

What we do differently:

To maintain consistent phenotypic properties for each of the cell lines, the following SOPs are standard practice at CH3:

  • Cells are always passaged or frozen in cryo-protectant when culture vessels reach 85 to 90% confluence.
  • Cells have never been exposed to trypsin; neither animal-derived nor recombinant.
  • Cells are always dislodged from the cell culture surface using trituration.
  • Superior technical support including individualized best practice guidelines for the cell culture of each cell line.

What cell lines can be licensed through CH3 BioSystems?

Well-established CH3 cell lines currently available are:

HEK293 human embryonic kidney cell line
HeLa human cervical cancer cell line
HepG2 human hepatoma hepatocellular carcinoma cell line
LNCaP human prostate cancer cell line
PC-3 human prostate cancer cell line
PC12 rat pheochromocytoma (original and variant cell lines)
E0771 murine breast cancer adenocarcinoma

Figure 2: HEK293 Cells

Featured Products

E0771 Cell Line – Murine Breast Cancer Cell Line

CH3 Cell Lines

PC12 Cell Line – Rat Pheochromocytoma

CH3 Cell Lines

Inhibitor A – Serine Protease Inhibitor

CH3 Enzymes

Video Library

How to Passage Cultured Cells Using Trituration:

Introduction to Flask Delivery:

Proper Procedure For Thawing Your Cell Line:

Your Cells Count! CH3 ensures plenty of cells are in each vial to get experiments running quickly:

Cell Line Authentication And Testing

If you need to authenticate your cell line for an NIH grant, or if you are looking to satisfy your IACUC prior to clearance of your transplantation studies, contact us. You can even design your own test panel! We have a broad array of options for you (see below).

In addition, species-specific PCR evaluation is performed to rule out any contamination by rat, human, hamster or monkey cells. Screening for additional pathogens may be available upon request. Contact us for customized pricing.

Products

Name Cat.# Description
ANTI-mRG 800001 Methylarginine-Specific Antibody
Anti‐Survivin 2B 150001 Survivin 2B-Specific Antibody
ANTI-RG 810002 Control Antibody Reagent
ANTI-RG 810001 Control Antibody Reagent
Anti‐Survivin ΔEx3 150002 Survivin ΔEx3-Specific Antibody
Anti‐Survivin 3B 150004 Survivin 3B-Specific Antibody
ANTI-mRG 800002 Methylarginine-Specific Antibody
Name Cat.# Description
E0771 Breast Cancer Cell Line 94A001 Murine Breast Cancer Cell Line
HEK293 Cell Line 92A001 Human Embryonic Kidney
HepG2 Cell Line 93A001 Hepatoma G2, Human Hepatocellular Carcinoma
PC12 Cell Line 91A001 Rat Pheochromocytoma
CellSep 99A002 Cell Counting Reagent
HeLa 94A003 HeLa Cervical Cancer Cell Line
Rat Tail Collagen 99A001 Cell Culture Substratum
PC-3 94A012 PC-3 Prostate Cancer Cell Line
LNCaP 94A002 LNCaP Prostate Cancer Cell Line
PC12Mn Cell Line 91A003 Variant PC12 Cell Line
PC12-C41 Cell Line 91A002 Variant PC12 Cell Line
Name Cat.# Description
PRMT7 820002 Type III Protein Arginine Methyltransferase
PRMT1 820001 Type I Protein Arginine Methyltransferase
S-Adenosylmethionine 890001 PH-Stabilized Methyl Donor
Name Cat.# Description
Arginine-Rich Peptide 830001 Methylation Substrate
Arginine-Rich Peptide 830002 Methylation Substrate