Hybridoma Reagent Laboratory's mission is to produce well-documented murine monoclonal antibodies specific for the human immunoglobulins (IgG PAN, IgG1, IgG2, IgG3, IgG4, IgM, IgA and IgE), the human light chains (kappa and lambda) and human secretory component.

These reagents are provided as capture antibodies for use in capture based immunoassays and as biotin or peroxidase-conjugated detection antibodies for use in non-isotopic immunoassays of human antibodies.

Product Categories

Product Overview

Product Code
(Clone Number)
Mouse Isotype Monoclonal Antibody Specificity pI Spectrotype [Range/Mean]
HP6017 ** IgG2a Human IgG Fc PAN (1,2,3,4) 7.4-7.8 (7.6)
HP6043 ** IgG1 Human IgG Fc PAN (1,2,3,4) ND
HP6046 IgG1 Human IgG Fd PAN (1,2,3,4) 6.1-6.6 (6.3)
Fd = heavy chain of Fab’2 fragment
HP6069 ** IgG1 Human IgG1 Fc 6.0-6.4 (6.2)
HP6070 IgG1 Human IgG1 Fc 6.1-6.8 (6.4)
HP6002 ** IgG1 Human IgG2 Fc 6.8-7.4 (7.1)
HP6014 ** IgG1 Human IgG2 Fd 6.5-6.7 (6.6)
HP6047 ** IgG1 Human IgG3 Hinge 6.5-6.7 (6.6)
HP6050 IgG1 Human IgG3 Hinge 6.5-6.9 (6.7)
HP6023 ** IgG3 Human IgG4 Fc 7.5-7.9 (7.7)
HP6025 IgG1 Human IgG4 Fc 6.2-6.5 (6.3)
HP6019 IgG1 Human IgG1,3,4 Exclusion
HP6030 IgG1 Human IgG2,3,4 Exclusion
HP6058 IgG1 Human IgG1,2,3 Exclusion
HP6029 IgG1 Human IgE Fc 7.3-7.5 (7.4)
HP6061 ** IgM Human IgE Fc ND
HP6083 ** IgG1 Human IgM Fc 6.1-6.4 (6.2)
HP6084 IgG1 Human IgM Fc 6.0-6.4 (6.2)
HP6111 IgG1 Human IgA Fc PAN (A1/2) ND
HP6123 ** IgG1 Human IgA Fd PAN (A1/2) ND
HP6130 IgG1 Human secretory IgA/SC* ND
HP6141 IgG1 Human secretory IgA/SC* ND
HP6062 IgG3 Human Kappa ND
HP6054 IgG2a Human Lambda 6.8-7.2 (7.0)


* SC = secretory component, ND = not done, HP6019-HP6030-HP6058 are exclusion antibodies because they bind to three of four human IgG subclasses.

** Most popular clones for their respective specificities.

Monoclonal Antibody Products

Product Code
(Clone Number)
Monoclonal Antibody Specificity Purified
2 mg/ml per ml
1 mg/ml per 0.5 ml
HRP Conjugated
1 mg/ml per 0.5 ml
HP6017  Human IgG Fc PAN (1,2,3,4) 6017P 6017B NA
HP6043  Human IgG Fc PAN (1,2,3,4) 6043P 6043B 6043HRP
HP6046 Human IgG Fd PAN (1,2,3,4) 6046P 6046B NA
HP6069 Human IgG1 Fc 6069P 6069B NA
HP6070 Human IgG1 Fc 6070P 6070B NA
HP6002 Human IgG2 Fc 6002P 6002B NA
HP6014 Human IgG2 Fd 6014P 6014B NA
HP6047 Human IgG3 Hinge 6047P 6047B NA
HP6050 Human IgG3 Hinge 6050P 6050B NA
HP6023 Human IgG4 Fc 6023P 6023B NA
HP6025 Human IgG4 Fc 6025P 6025B NA
HP6019 Human IgG1,3,4 6019P 6019B NA
HP6030 Human IgG2,3,4 6030P 6030B NA
HP6058 Human IgG1,2,3 6058P 6058B NA
HP6029 Human IgE Fc 6029P 6029B NA
HP6061 Human IgE Fc 6061P 6061B NA
HP6083 Human IgM Fc 6083P 6083B 6083HRP
HP6084 Human IgM Fc 6084P 6084B NA
HP6111 Human IgA Fc PAN (A1/2) 6111P 6111B NA
HP6123 Human IgA Fd PAN (A1/2) 6123P 6123B 6123HRP
HP6130 Human secretory IgA/SC* 6130P 6130B NA
HP6141 Human secretory IgA/SC* 6141P 6141B NA
HP6062 Human Kappa 6062P 6062B NA
HP6054 Human Lambda 6054P 6054B NA

* SC = secretory component, NA = not available.

Monoclonal Antibody Based Enzyme Immunoassays

For the Human Immunoglobulins

Human Isotype EIA Capture Antibody Detection Antibody
Total Human IgG HP6046P HP6043-HRP
Total Human IgG HP6046P PolyMono anti-human IgG *
Total Human IgG1 HP6069P PolyMono anti-human IgG*
Anti-Kappa/Lambda MAb *
Total Human IgG2 HP6002P PolyMono anti-human IgG*
Anti-Kappa/Lambda MAb *
Total Human IgG3 HP6047P PolyMono anti-human IgG*
Anti-Kappa/Lambda MAb *
Total Human IgG4 HP6025P PolyMono anti-human IgG*
Anti-Kappa/Lambda MAb *
Total Human IgE HP6061P HP6029-Biotin
Total Human IgA HP6111P HP6123-Biotin
Total Secretory IgA HP6111P HP6130-Biotin
Total Human IgM HP6084P HP6083-Biotin
Total Human IgM HP6084P HP6083-HRP

*The human IgG subclass enzyme immunoassays may be developed with a biotinylated poly-monoclonal anti-human IgG reagent, anti-kappa/lambda light chain reagent or a polyclonal mouse anti-human IgG reagent.

Capture antibodies are to be used at 10 micrograms per ml (1:200 dilution) in PBS-no protein for coating and detection antibodies are to be used at 1 microgram per ml (1:1000 dilution) in PBS-1% bovine serum albumin.

Horseradish Peroxidase Labeled Monoclonal Antibodies

Monoclonal antibodies specific for human IgG PAN (IgG1/2/3/4), IgA PAN (IgA1/2) and human IgM have been directly conjugated with horseradish peroxidase (HRP) using the Nakane/Kawaoi procedure. These antibodies are supplied at 1 mg/ml in 0.5 ml aliquots and should be diluted from 1:2000 to 1:8000 for use in immunoassays. These antibodies are being used in an internationally documented protocol for the measurement of human IgG, IgA and IgM antibodies specific for pneumococcal polysaccharides.

HP6043anti-human IgG Fc
HP6083anti-human IgM Fc
HP6123anti-human IgA Fd


Selected References Related to or Using HRL Monoclonal Antibodies

  1. Jefferis R., CB Reimer, F Skvaril et al. Evaluation of monoclonal antibodies having specificity for human IgG subclasses. Results of an IUIS/WHO collaborative study. Immunol Lett 10:223, 1985.
  2. Reimer CB et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3:236, 1984.
  3. Phillips DJ, TW Wells, CB Reimer. Estimation of association constants of monoclonal antibodies to human IgG epitopes using fluorescent sequential saturation assays. Immunol Lett 17:159, 1987.
  4. Hamilton RG. Human IgG subclass measurements in the clinical laboratory. Clin Chem 33:1070-5, 1987.
  5. Hamilton RG. Human IgG Subclasses. Monograph, Calbiochem Corporation, pp 1-76, 1987.
  6. Hamilton RG, RW Wilson, T Spillman, M Roebber. Monoclonal antibody-based immunoenzymetric assays for quantification of human IgG and its four subclasses. J Immunoassay 9:275-96, 1988.
  7. Hamilton RG, M Roebber, CB Reimer, SL Rodkey. Isoelectric focusing patterns of mouse monoclonal antibodies to the four human IgG subclasses. Electrophoresis 9:127-34, 1987.
  8. Hamilton RG, M Roebber, CB Reimer, SL Rodkey. Quality control of murine monoclonal antibodies using isoelectric focusing immunoblot analysis. Hybridoma 6:204-217, 1987.
  9. Reimer CB. Five hydridomas secreting monoclonal antibodies against human IgE. Monoclonal Antibody News 4:2, 1986.
  10. Jones CC, RG Hamilton, RE Jordon. Subclass distribution of human IgG autoantibodies in pemphigus. J Clin Immunol 8:43-9, 1988.
  11. Fasullo FJ Jr, HA Fritsche Jr, F Liu, RG Hamilton. IgG heavy chain subclass typing of myeloma paraprotein by isoelectric focusing (IEF) and immunoblot analysis. Clin Chem 35:364-8, 1989.
  12. Hamilton RG. Inter-laboratory quality control program for IgG subclass protein immunoassays in North America. In. Protides of the Biological Fluids, Pergamon Press, 1989.
  13. Reimer CB, DJ Phillips, CH Aloisio, CM Black, TW Wells. Specificity and association constants of 33 monoclonal antibodies to human IgA epitopes. Immunol Lett 21:209, 1989.
  14. Hamilton RG. Engineered human antibodies as immunologic quality control reagents. Ann Biol Clin 48:473-7, 1990.
  15. Hamilton RG. Production and epitope location of monoclonal antibodies to the human IgG subclasses. In: Molecular Aspects of Immunoglobulin Subclasses. ed. F Shakib, Pergamon Press, Oxford, pp 79-91 (Chapter 5), 1990.
  16. Hamilton RG. Application of engineered chimeric antibodies to the calibration of human antibody standards. Ann Biol Clin. 49:242-248, 1991.
  17. Herrman DJ, RG Hamilton, T Barington CE Frash, G Arakere, O Makela, LA Mitchell, J Nael, GT Rijkers, B Zegers, B Danve, JI Ward, CS Brown. Quantitation of human IgG subclass antibodies to Haemophilus influenzae Type b capsular polysaccharide: Results of an international collaborative study using enzyme immunoassay methodology. J Imunol Meth 148:101-14, 1992.
  18. Lichtenstein LM, A Kagey-Sobotka, JM White, RG Hamilton. Anti-human IgG causes basophil histamine release by acting on IgG-IgE complexes bound to IgE receptors. J Immunol 148:3929-36, 1992.
  19. Wilson ME, RG Hamilton. IgG subclass response of localized juvenile periodontitis subjects to Actinobacillus actinomycetemcomitans Y4 lipopolysaccharide. Infection Immunity 60:1806-12, 1992.
  20. Hamilton RG, SM Morrison. Epitope mapping of human IgG specific murine monoclonal antibodies with domain-switched, deleted and point-mutated chimeric antibodies. J Immunol Meth 158:107-22, 1993.
  21. Batard T, B Basuyaux, P Lambin, C Bremard-Oury, RG Hamilton, B David, G Peltre. Isotypic analysis of grass pollen-specific immunoglobulins in human plasma. I. Specialization of certain classes and subclasses in the immune response. Intern Arch Allergy Immunol 10:68-73, 1993.
  22. Kuppers RC, IM Outschoorn, RG Hamilton, CL Burek, NR Rose. Quantitative measurement of human thyroglobulin-specific antibodies by use of a sensitive enzyme-linked immunoassay. Clin Immunol Immunopath 67:1-10, 1993.
  23. Peebles S, M Liu, LM Lichtenstein, RG HamiltonIgA, IgG and IgM quantification in bronchoalveolar lavage fluids from allergic rhinitics, allergic asthmatics and normal subjects by monoclonal antibody-based immunoenzymetric assays. J. Immunol Meth. 179:77-86, 1995.
  24. Wilson ME, RG Hamilton. IgG2 antibodies promote neutrophil killing of Actinobacillus actinomycetemcomitansInfection Immunity 63:1070-1075, 1995.
  25. Kleine-Tebbe J, RG Hamilton, M. Roebber, LM Lichtenstein, SM MacDonald. Purification of immunoglobulin E (IgE) antibodies from sera of high IgE titers. J. Immunol Meth. 179:153-164, 1995.
  26. Wilson ME, RG Hamilton. IgG subclass response on juvenile periodontitis subjects to principal outer membrane proteins of Actinobacillus actinomycetemcomitansInfection Immunity 63:1062-1069, 1995.
  27. Madore DV, P Anderson, BD Baxter, GM Carlone, KM Edwards, RG Hamilton. Inter-laboratory study evaluating quantitation of antibodies to Haemophilus influenzae type b polysaccharide by ELISA. Clinical and Diagnostic Laboratory Immunology 3:84-88, 1996.
  28. Mestecky J. RG. Hamilton, CGM Magnusson, R Jefferis, JP Vaerman, M Goodall, GG de Lange, I Moro, P Aucouturier, J Radl, C Cambiaso, C Silvain, JL Preud’homme, K Kusama, GM Carlone, J Biewenga, K Kobayashi, CB Reimer. Evaluation of monoclonal antibodies with specificity for human IgA, IgA subclasses and allotypes and secretory component. Results of an IUIS/WHO collaborative study. J. Immunol. Meth. 193(2):103-148, 1996.
  29. Hamilton RG. N.F. Adkinson Jr. Immunological tests for the diagnosis and management of human allergic disease: Total and allergen-specific IgE and allergen specific IgG. In: Manual of Clinical Laboratory Immunology. ed. HR Rose, EC de Macario, JL Fahey, H Friedman, GM Penn. American Society for Microbiology, Washington D.C., Chapter 102, 1997.
  30. Peebles RS, MC Liu, NF Adkinson Jr., LM Lichtenstein, RG Hamilton. Ragweed specific antibodies in bronchoalveolar lavage fluids and serum before and after segmental lung challenge: IgE and IgA associated with eosinophil degranulation. J Allergy Clin Immunol 101:265-73, 1998.
  31. King CJ, W Borkowsky, N Mahidhara, ED Shapiro, RM Rutstein, NQ Tan, JJ Farley, WM Dankner, S Nachman, E Simoes, PM Flynn, J Clemens, A Kimura, RG Hamilton. Group specific antibody levels surrounding invasive pneumococcal illness in children infected with human immunodeficiency virus. J. Infect. Dis. 181-1817-21, 2000.
  32. Peebles RS, RG Hamilton, LM Lichtenstein, M Schlosberg, MC Liu, D Proud, A Togias. Antigen specific IgE and IgA antibodies in bronchoalveolar lavage fluid are associated with stronger antigen-induced late phase reactions. Clin. Exp. Allergy 31:239-48,2001.

First for Antibodies

Stratech Scientific have been successful in a recent antibodies tender from the Southern Universities Purchasing Consortium. In the award criterion Read More…