MethylDetect’s goal is to make the MS-HRM technology easy accessible both for new-comers and experienced researchers in the field of epigenetics.
We offer kits for DNA methylation detection that are ready-to-use and compatible with standard laboratory equipment

Product Panels

Apoptosis DAPK1 TNFRSF25(DR3) TNFRSF21­
Breast Cancer BRCA1 BRCA2 TIMP3
Colon cancer ZNF442 WRN VIM
Gastric Cancer TIMP3 GSTP1 DAPK1
Leukemia and Lymphoma GSTP1 DAPK1 LATS2
Liver Cancer TIMP3 GSTP1 DAPK1
Melanoma TIMP3 DAPK1 CCND2
Prostate Cancer TIMP3 GSTP1 PITX2
Cell cycle TP53 RBL1 RAD9A
Cytokine Production TRAF2 TRAF6 TLR2
DNA Methylation Assay P16 SEPT9 TIMP3
Homeobox Genes (HOX) LHX1 PITX2 SIX6
Inflammatory response and autoimmunity ABCF1 TYK2 TRAF2
Mental Disorders TERT TFAM SORBS3
Notch Signaling Pathway SNW1 RBPJL PSENEN
Polycomb Genes PcG TRIM27 SUZ12 RING1
Stem Cell Transcription Factors EGFR PITX2 WT1
Stress response and Toxicity XRCC6(G22P1) XBP1 XRCC2
T Helper Cell Differentiation TNFSF11 TGIF1 TBX21
T-Cell and B-Cell Activation THY1 SOCS1 NCK1
TGFbBMP Signaling Pathway TGFB1 TGFB2 TGFB3
TollLike Receptor TLR Signaling UBE2V1 TRAF6 TOLLIP
Tumor Suppressor Genes TSG APC MGMT RASSF1A
WNT Signaling WNT10A WNT10B WNT5A

DNA Methylation Testing Kits

DNA Methylation is one of the most widely studied epigenetic mechanisms of gene expression regulation. Aberrant methylation of increasing number of genes is being shown to contribute to the majority of diseases such as cancer, cardiovascular disorders, aging, obesity, allergy, diabetes, depression, Alzheimer’s, Parkinson’s or schizophrenia.
Solid research evidence already shows that DNA methylation-based biomarkers can be utilized at all stages of clinical disease management including:

  • Disease risk assessment
  • Early detection
  • Personalization of treatment
  • Monitoring of chronic disorders and recurrence

The technology was already used for the detection of methylation biomarkers in fields like forensics and such a difficult DNA material sources as liquid biopsies or FFPE tissues.
MethylDetect offers ready-to-use kits for detection of aberrant gene methylation. The kits are based on the MS-HRM (Methylation Sensitive High Resolution Melting) technology and can be used with standard laboratory equipment. Additionally, our new controls system shipped with each kit, provides state-of-the-art experimental reliability.

Methylation detection with MethylDetect’s Kit

1. ADD: The MethylDetect kit components to the bisulfite-modified biological sample and PCR reagents
2. AMPLIFY: Use standard laboratory Real Time-PCR system to PCR amplify the target
3. DETECT: Assess the methylation status of the sequence of interest with a High-Resolution Melting module

Principles of methylation detection with MethyDetect Kit