Microsurfaces

MicroSurfaces Inc. develop surface coating technologies for microarray, microfluidic and biosensor applications.

Their proprietary core technology is derived from chemical processes which allow the control and engineering of surface properties to immobilise biomolecules in particular proteins.

MSI provide functional coatings on glass slides, covers slips, silicon wafers and other surfaces.

Their most recent development is a new class of robust PEG surface coatings that can withstand harsh experimental conditions.


Product Categories  

Microcell arrays

  • MICROGRID ARRAYS
  • MICROWELL ARRAYS
  • MICROMESH ARRAYS
  • MICROEB ARRAYS

Custom designs

If you require a specific size, thickness or profile that is not listed please contact us to create a custom array for you.

  • CUSTOM SIZE OR SHAPE
  • NOVEL MATERIALS

Tips & Tricks

MICROCELL ARRAY NOT STICKING TO THE CELL CULTURE WARE

  1. Inspect the quality of the insert’s seal against the cell culture ware by looking through the bottom of the cell culture ware. The presence of air bubbles will reduce the sticking performance of the insert. Remove the insert and try again.
  2. If no air bubbles are present and the insert will not stick to the cell culture ware surface, then remove the insert from the cell culture ware.
  3. Carefully place the bottom surface of the microcell array onto some 3M scotch tape. Note general sticky tape will not work as it can leave glue residue on the insert. The insert can aggresively collect dust/oils that reduces its ability to self-stick to a surface. The scotch tape removes the dust/oils enabling the insert to stick to the culture ware.
  4. Using a pair of tweezers carefully peel the insert off the tape and place it onto a clean section of tape.
  5. Repeat step 3 at least 3 times.
  6. Return the microcell array to the cell culture ware. Make sure that the insert is only ever applied to a dry surface.

IRREVERSIBLY BONDING MICROCELL ARRAY INSERT TO A GLASS SURFACE

  1. Clean the insert and glass surface of the cell culture ware in ethanol and dry with compressed air or nitrogen. Note the inserts will only bond with a glass surface.
  2. Activate the surfaces of the insert and glass cell culture ware using an Oxygen plasma or corona discharge for approximately 15 seconds.
  3. Carefully place the insert onto the glass surface and press down on all areas of the insert making sure it is in contact with the glass. Note if you have made a mistake or a bubble has become trapped between the insert and glass surface you only have one to two minutes to remove the insert and try again before it bonds with the glass.
  4. Hold the insert in place for 30 seconds with pressure from your finger or thumb.
  5. Wait at least 1 hour before starting the ethanol rinse (step 3 in the protocol for using microcell arrays).

MATLAB TOOL BOX FOR PROCESSING TIME LAPSE MOVIES OF CELLS
John Markham from the National ICT Australia (NICTA) has developed a MATLAB based tool box called MATS (Microgrid Array Tools) to process time lapse microscopy experiments using microgrid arrays. Unlike other processing software MATS has some specific features for use with microgrid arrays like;

  1. Automatic recognition of microgrid boudaries. There are three algorithms which can be used with transmission images to find the microgrid boundaries.
  2. Support for spectral unmixing based on the Zeiss Colibri illumination with a quad-band filter block.
  3. Support for automatic position setting. Setting positions that cover all of the 1,000’s of wells in a microgrid array is a painful task, so a script is included that automates it, including doing interpolation of the focus to account for a non-level holder.
  4. Support for measurement of FUCCI cell cycle reporter proteins.
  5. Support for multi-treading and GPU acceleration, which allows you to use as many cores on your local machine as the MATLAB parallel tool box will support.
  6. Generation of position specific background images. MATS uses all the images in a stack to try and find the background. THis inly works if the cells are motile and take up only a small proportion of the image.
  7. Support for image registration to account for misalignment at successive time points.

MSI provides functional coatings on glass slides, coverslips, silicon wafers, nanoparticles, and other surfaces for the immobilisation of biomolecules, including customised coating services.  MSI’s glass slide has distinct advantages over current glass slides on the market.  This advantage is reflected in its exceptionally low background, high uniformity, and high chemical reactivity.  MSI has combined novel technologies from both surface chemistry and protein chemistry to create a class of high quality functional glass slides and surface modification technologies for microfluidic devices.

MSI also offer high throughput screening (HTS) services of compound libraries, drug candidates and nanoparticle-based medicine.  They specialise in the HTS of cell surface interactions in proteomics and glycomics analysis using their FluidArray® cell-membrane microarray platform.


Vision

A COLLABORATIVE APPROACH

Our goal is to continually develop innovative surfaces and devices that enable our customers to achieve breakthroughs in their work, whether it be in research or industry. This commitment to innovation is driven through creating partnerships with our customers and utilising their expertise and feedback to deliver the future in cell culture ware.

LEADING EDGE PRODUCTS

The aim of Microsurfaces is to become a global manufacturer of innovative high content imaging technologies that enable our customers to achieve breakthroughs in performance, quality and excellence.


History

2017 –

Microsurfaces officially launches the improved microwell array and new microEB array product lines.

2013 –

Microsurfaces officially launches the micromesh array and microwell array product lines. In addtion the new website is released.

2011 –

Microsurfaces Pty Ltd was founded by Dr Daniel Day to manufacture the microgrid array inserts.

2010 –

Biosurfaces Pty Ltd was established as a University start-up company in collaboration with the Cooperative Research Centre for Polymers to commercialise the microstructure fabrication processes for creating biological cell culture ware.

2009 –

Patented process for ‘Method for fabricating microstructured cell culture devices’.

2007 –

Patented process for ‘Replica moulding of microstructures for supporting microscopic biological material’.

2005 –

Initial fabrication experiments for creating microstrucuted surfaces for containing cells during laser transfection.


Reviews

Microsurfaces ZeroBkg PEG coverslips from Stratech, Testimonial

“Spectacular decrease in background compared to regular coverslips, giving us the outstanding signal-to-noise ratio we need for sensitive single-molecule experiments”

Dr Anthony Roberts, Sir Henry Dale Fellow and Group Leader, ISMB, Birkbeck/UCL

Air-Stable Lipid Bilayers

  Fig. 1. Forming fluidic & air-stable supported lipid bilayers on the FluidicArray surface Fig. 2. Fluorescence recovery after photobleaching (FRAP) Read More…

ZeroBkg® Functional Surfaces

Proteins tend to adsorb nonspecifically to most solid surfaces. This is because a protein molecule has various hydrophobic domains, charged sites, Read More…

Silane surfaces

Application Note: We have turned silane coating, the “black magic”, into a reproducible and quality controlled silane monolayer on a Read More…

Polylysine surfaces

Application Note: We have optimized the fabrication of polylysine coatings on glass, quartz, and other hydrophilic surfaces. Our polylysine coatings Read More…