SynVivo

SynVivo’s proprietary microfluidic chips are capable of supporting a microvascular network that simulates the circulation inside any tissue with respect to flow, shear and pressure. Novel co-culture protocols have been developed that establish true vascular monolayers in communication with tissue cells. Human cells grown in SynVivo chips retain biological phenotypes that are similar to cells found in the tissues. Leading researchers have validated that cells grown in SynVivo chips more accurately reflect the tissue cells found inside the body than do cells grown using conventional culture techniques.

The successful coupling of digitized tissue imaging with silicon etching technologies allows SynVivo to design and manufacture microfluidic chips that can be adapted for a variety of uses. All chip designs incorporate ports for introduction of cells and reagents and for collecting effluent for analysis. They can accommodate virtually any analytical technology.


 

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3D Tissue Models

3D tissue models from SynVivo enable real-time study of cell and drug interactions and accelerate discovery by providing a biologically realistic platform that more accurately depicts in vivo reality.

Microfluidic Chips

SynVivo microfluidic chips are readily available as standard stock items. We are also capable of producing any custom designed chip and morphology as needed. Contact us for more information on customization.

Instruments

In order to run microfluidic chips you need some basic lab equipment and instruments. We are able to offer pumps, stages, and scopes to get your lab up and running!

Accessories

We offer a full line of supplies and accessories to support your research.


SynVivo is a physiological, cell-based microchip platform that provides a morphologically and biologically realistic microenvironment allowing real-time study of cellular behavior, drug delivery and drug discovery.

Technology

SynVivo’s patented technology for recreating microvasculature scale, shape, fluidics, and cellular constructs provides the most realistic in vitro testing platform for drug discovery, delivery, efficacy, and toxicity assays.Static well plate and linear flow channel assays cannot differentiate between shapes of drug delivery vehicles, whereas SynVivo can. As particle shape becomes a more relevant area of study for drug delivery, SynVivo can provide new insights that can help optimize drug delivery.

What it Does

SynVivo can be used to capture real-time distribution of drugs in the microvasculature. Stagnant and recirculation zones that affect overall drug delivery and distribution can be readily identified.

SynVivo can be used to quantify drug particle distribution patterns through analysis of shear rate and adhesion.

SynVivo can support the growth of endothelial cells, cancer cells and many other cell types for mimicking healthy and disease conditions. In addition, targeted drug delivery can be quantified and optimized using the in vivo morphology and fluidic conditions in SynVivo.

 



How it’s Made

Starting with the scans of animal or human microvasculature networks, SynVivo creates a replica of the network on a microchip. Within this morphologically realistic network, animal or human cells are cultured and studied under physiologically realistic flow and shear conditions. For advanced studies, tumor or tissue cells can be co-cultured within and around the microvasculature network.

The platform comprises a disposable chip that is imprinted with microvascular networks, a pump to perfuse cells, reagents, and solutions, and customized software for analysis of experimental data.


 

Validation of SynVivo Assay

Example of SynVivo results can found at:

 


New publications using the Synvivo platform for drug delivery and therapeutic screening applications

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3D TISSUE MODELS AND SERVICES-BROCHURE   PKCδ inhibition as a novel medical countermeasure for radiation-induced vascular damage; Fariborz Soroush, Yuan Tang, Hasan M. Zaidi, Joel B. Sheffield, Laurie E. Kilpatrick, and Mohammad F. Kiani   Elucidating the Influences of Size, Surface Chemistry, and Dynamic Flow on Cellular Association of Nanoparticles Made by Polymerization‐Induced Self‐Assembly; Song Yang Khor, Mai N. Vu, Emily H. Pilkington, Angus P. R. Johnston, Michael R. Whittaker, John F. Quinn, Nghia P. Truong, Thomas P. Davis   A Microvascularized Tumor-mimetic Platform for Assessing Anti-cancer Drug Efficacy. Shantanu Pradhan, Ashley M. Smith, Charles J. Garson, Iman Hassani, Wen J. Seeto, Kapil Pant, Robert D. Arnold, Balabhaskar Prabhakarpandian & Elizabeth A. Lipke. Scientific Reports Volume 8, Article number: 3171(2018)

 
 
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Trastuzumab Distribution in an In-Vivo and In-Vitro Model of Brain Metastases of Breast Cancer Tori B. Terrell-Hall, Mohamed Ismail Nounou, Fatema El-Amrawy, Jessica I.G. Griffith and Paul R. Lockman Oncotarget. 2017; 8:83734-83744

A Biomimetic Microfluidic Tumor Microenvironment Platform Mimicking the EPR Effect for Rapid Screening of Drug Delivery Systems Yuan Tang, Fariborz Soroush, Joel B. Sheffield, Bin Wang, Balabhaskar Prabhakarpandian & Mohammad F. Kiani Scientific Reports 7, Article number: 9359 (2017)

Adhesion Patterns in the Microvasculature are Dependent on Bifurcation Angle. G. Lamberti, F. Soroush, A. Smith, M. Kiani, B. Prabhakarpandian, K. Pant. Microvascular Res., 2015, 99, pp 19-25

Expanding Imaging Capabilities for Microfluidics: Applicability of Darkfield Internal Reflection Illumination (DIRI) to Observations in Microfluidics. Y. Kawano, C. Otsuka, J. Sanzo, C. Higgins, T. Nirei, T. Schilling, T. Ishikawa. PLoS ONE, 2015, 10(3): e0116925
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Microfluidic co-culture devices to assess penetration of nanoparticles into cancer cell mass. Jarvis M, Arnold M, Ott J, Pant K, Prabhakarpandian B, Mitragotri S. Bioeng Transl Med. 2017 Sep 26;2(3):268-277   Permeability across a novel microfluidic blood‑tumor barrier model. Tori B. Terrell‑Hall , Amanda G. Ammer , Jessica I. G. Griffith and Paul R. Lockman Fluids and Barriers of the CNS (2017) 14:3   A novel microfluidic assay reveals a key role for protein kinase C δ in regulating human neutrophil-endothelium interaction. Soroush F, Zhang T, King DJ, Tang Y, Deosarkar S, Prabhakarpandian B, Kilpatrick LE, Kiani MF. J Leukoc Biol November 2016 100:1027–1035.   A Novel Dynamic Neonatal Blood-Brain Barrier on a Chip. S. Deosarkar, B. Prabhakarpandian, B. Wang, J.B. Sheffield, B. Krynska, M. Kiani. PLOS ONE, 2015
Synthetic Tumor Networks for Screening Drug Delivery Systems. B. Prabhakarpandian, MC Shen, J. Nichols, C. Garson, I. Mills, M. Matar, J. Fewell, K. Pant. J Control Release., 2015, 201, 49-55   Bioinspired Microfluidic Assay for In Vitro Modeling of Leukocyte–Endothelium Interactions. G. Lamberti, B. Prabhakarpandian, C. Garson, A. Smith, K. Pant, B. Wang, and M.F. Kiani. Anal. Chem., 2014, 86 (16), pp 8344–8351   Generation of Shear Adhesion Map Using SynVivo Synthetic Microvascular Networks. Smith, A. M., Prabhakarpandian, B., Pant, K. J. Vis. Exp. (87), e51025, 2014   Using shape effects to target antibody-coated nanoparticles to lung and brain endothelium. Kolhara P, Anselmob AC, Guptab V, Pant K, Prabhakarpandian B, Ruoslahtid E, and Mitragotri S. PNAS 2013

 
 
 
Adhesive Interaction of Functionalized Particles and Endothelium in Idealized Microvascular Networks. G. Lamberti, Y. Tang, B. Prabhakarpandian, Y. Wang, K. Pant, M.F, Kiani, B. Wang. Microvascular Res. 2013 (89) pp 107-114

SyM-BBB: A Microfluidic Blood Brain Barrier Model B. Prabhakarpandian, M.-C. Shen, J.B. Nichols, I.R. Mills, M.S.-Wegrzynowicz, M. Aschner, K. Pant, Lab on a Chip, 2013, 13, 1093-1101

A physiologically realistic in vitro model of microvascular networks. Rosano JM, Tousi N, Scott RC, Krynska B, Rizzo V, Prabhakarpandian B, Pant K, Sundaram S, Kiani MF. Biomed Microdevices. 2009 May 19

Synthetic microvascular networks for quantitative analysis of particle adhesion. Prabhakarpandian B, Pant K, Scott RC, Patillo CB, Irimia D, Kiani MF, Sundaram S. Biomed Microdevices. 2008 Aug;10 (4):585-95
Microfluidic devices for modeling cell-cell and particle-cell interactions in the microvasculature. Prabhakarpandian B, Shen MC, Pant K, Kiani MF. Microvasc Res. 2011 Nov;82(3):210-20   Bifurcations: focal points of particle adhesion in microvascular networks. Prabhakarpandian B, Wang Y, Rea-Ramsey A, Sundaram S, Kiani MF, Pant K. Microcirculation. 2011 Jul;18(5):380-9   Flow and adhesion of drug carriers in blood vessels depend on their shape: a study using model synthetic microvascular networks. Doshi N, Prabhakarpandian B, Rea-Ramsey A, Pant K, Sundaram S, Mitragotri S. J Control Release. 2010 Sep 1;146(2):196-200   Preferential adhesion of leukocytes near bifurcations is endothelium independent. Tousi N, Wang B, Pant K, Kiani MF, Prabhakarpandian B. Microvasc Res. 2010 Dec;80(3):384-8

Application: Adding a New Dimension to Your Migration Assays

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SynVivo for CytoViva

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Realistic and Dynamic In Vitro Model of the Blood Brain Barrier

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SynVivo Videos

  Endothelial Cell Division Under Flow Time lapse video (~24 hours) of primary endothelial cells undergoing cell division and morphological Read More…

Resisting Cell Death

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A Better Model for the Blood-Brain Barrier

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Stratech Scientific showcases SynVivo at the ELRIG‬ Conference in Telford

Stratech‬ Scientific Sales Manager (Gareth Bolitho) yesterday gave a talk at the annual “Dragons Den” event at ELRIG‬ showcasing Synvivo‬ ‪’s Read More…

Leukocyte Rolling, Adhesion, and Migration in a Single Assay!

Leukocytes play a key role in early response to tissue injury/infection resulting from physical, chemical or biological stimuli. This process Read More…