A. All PBL ELISA kits are constructed with carefully selected proprietary antibodies and optimized reagents.
A. To optimize each lot of kits, PBL modifies the concentration of the to achieve a standard curve that will meet PBL specific range and signal to noise requirements. In addition we have criteria for: Intra Assay: %CV of replicate wells, %RE of back calculated concentration; and Inter Assay: %CV of back calculated concentration..
A. No. Reagents concentrations are optimized according to lot. However, components from different kit lots may be used if the component lot is the same. (note: this is especially true for TMB and Stop solutions since the same lot may be used for multiple kit lots).
A. With the exception of Human Interferon Alpha Serum ELISA kits (product # 41110-1 and 41110-2) and Mouse Interferon Beta ELISA kit (product # 42400-1 and 42400-2), all other PBL ELISA kits are not characterized for use with serum samples.
A. User cell lines may vary so there is no specific answer to this question. However, the general rule is a 70 fold excess in antibody mass should be used for neutralizing interferon. It is recommended to quantify the amount of interferon via techniques such as ELISA. It is also recommended to allow time (1-2 hPBLs in general) for the antibody to neutralize the interferon before adding the virus.
A. For monoclonal antibodies, use normal antibody from the same species and the same isotype. For example, if a MAb is a murine IgG2a, use the normal equivalent as the negative control. Many of PBL’s polyclonal antibodies are unpurified sera. Therefore, investigators should use normal unpurified serum from the appropriate species as their negative control.
A. PBL does not offer any controls for PBL antibodies at the current time.
A. Based on viral challenge assay. The assay measures the ability of the antibody to negate the protective affects of the interferon against the viral challenge.
A. Endotoxin level is < 0.1 U/µg. PBL limit is 1.0.
A. Products are sold with a carrier contain protein such as Bovine Serum Abumin (BSA) to ensure stability and prevent aggregates from forming. Products sold without carrier do not contain BSA. Carrier free products recommended in applications including in vivo (in vivo italicized) injection, conjugation, or surface binding assays. (Note: BSA endotoxin level is 0.1-1 U/ml).
A. The formula to use to convert S.A. to pg/ml is as follows:
(1 x 109 ÷ (Lot specific activity)) x (Lot Concentration (U/ml)= pg/ml.)
A. Acetic acid was added to prevent aggregation of interferon beta proteins.
A. Refer to product insert for appropriate dilution buffer.