4E Binding Protein 1 Antibody Kit (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

Catalogue Number: 0004-18C-USB

Manufacturer:	United States Biological
Physical state:	Supplied as a liquid in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA and 50% glycerol.
Type:	Antibody Packs
Shipping Condition:	Blue Ice
Unit(s):	6x20 ul
Host name:
Clone:
Isotype:	IgG
Immunogen:	Synthetic phosphopeptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1, residues surrounding Thr46 of human 4E-BP1, residues surrounding Ser65 of mouse 4E-BP1 and derived from the sequence around Ser112 of human 4E-BP1.
Application:	ELISA, FC, ICC, IF, IP, WB, IHC

Overview
Datasheets

Additional Text

Caution

FOR RESEARCH USE ONLY

Gene ID

1978

Gene Name

EIF4EBP1

Uniprot ID

Q13541

Calc Crossreactivity

Bo Hu Mk Mo Rt

Immunogen

Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1, residues surrounding Thr46 of human 4E-BP1, residues surrounding Ser65 of mouse 4E-BP1 and derived from the sequence around Ser112 of human 4E-BP1.

Specificity

Detects endogenous levels of 4E-BP1 only when dephosphorylated at Thr46. This antibody cross-reacts with 4E-BP2 and 4E-BP3 dephosphorylated at equivalent sites. Phospho- 4E-BP1 (Ser65) antibody detects endogenous levels of 4EBP1 when phosphorylated at Ser65, and may also recognize 4E-BP1 when phosphorylated at Ser101. Phospho-4E-BP1 (Ser65) (174A9) rabbit Mab detects endogenous levels of 4E-BP1 when phosphorylated at Ser65. Phospho-4E-BP1 (Thr70) antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Thr70. 4E-BP1 (53H11) rabbit Mab detects endogenous levels of total 4E-BP1 protein. 4E-BP2 antibody detects endogenous levels of total 4E-BP2 independent of phosphorylation and does not cross-react significantly with 4E-BP1.

Purification

Affinity Purified; Protein A Purified

0004-18C Datasheet

Price

£909.00

-

Quantity
Unit Size

Find out about delivery