Mouse anti Human CD2
Catalogue Number: 0021-EXA
| Manufacturer: | ExAlpha |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | T-cell surface antigen CD2; CD2 cytoplasmic domain-binding protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | T6.3 |
| Isotype: | IgG2a |
| Immunogen: | |
| Application: | FC |
Description
Description: Mouse anti human CD2 can be used for identification of human T cells and a subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.
Additional Text
Gene Name
CD2
Gene ID
914
Uniprot ID
P06729
Purification
Protein A/G Purified
Antibody Clonality
Monoclonal
Application Notes
Flow cytometry: PBMC: Add 10 µl of antibody to 1 million PBMCs in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8oC. Wash twice with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of antibody to 100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles
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