Mouse anti Human CD2 FITC - CD7 PE
Catalogue Number: 0027-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Antibody Packs |
| Alias: | CD2 FITC - CD7 PE |
| Shipping Condition: | RT |
| Unit(s): | 100 tests |
| Host name: | Mouse |
| Clone: | |
| Isotype: | |
| Immunogen: | CD2=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with t lymphocytes activated by mixed lymphocyte culture. CD7=Derived from the hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells of BALB/c mice immunized with T-acute lymphoblastic leukemia (T-ALL) cells. |
| Application: | FC |
Description
Description: Identification of human T cells and subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen. Identification of human T lymphocytes in multiple stages of T cell development, including a major subset of mature peripheral T cell. CD7 antigen is often increased on T leukemic cells. The CD7 molecule is a 40,000 M.W. surface antigen that is expressed on T-Lymphoid and myeloid precursors in fetal liver and bone marrow.
Additional Text
Gene Name
CD2
Gene ID
914
Uniprot ID
P06729
Purification
Protein A/G Purified
Application Notes
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB /100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at 4-8C. DO NOT FREEZE
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