Mouse anti Human CD4, conjugated with Biotin

Catalogue Number: 0043-EXA

Manufacturer:	ExAlpha
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3
Shipping Condition:	Blue Ice
Unit(s):	100 tests
Host name:	Mouse
Clone:	7E14
Isotype:	IgG1
Immunogen:	Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
Application:	FC

Overview

Description

Description: Identification of human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes.

Additional Text

Gene Name

CD4

Gene ID

920

Uniprot ID

P01730

Purification

Protein A/G Purified

Antibody Clonality

Monoclonal

Application Notes

PBMC: Add10 µl of MAB/106 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2o to 8oC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN : (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5 +: Use 5 µl of solution, rather than 10 µl as above. Excites at 488nm and emits at 670nm. Store protected from light.

Storage Note

Product should be stored at 4-8C. DO NOT FREEZE

Price

£218.00

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