Mouse anti Human CD4 FITC - CD8 PE
Catalogue Number: 0048S-EXA
| Manufacturer: | ExAlpha |
| Type: | Primary Antibody |
| Alias: | CD4 FITC - CD8 PE |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 tests |
| Host name: | Mouse |
| Clone: | |
| Isotype: | IgG1 |
| Immunogen: | CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes. |
| Application: | FC |
Description
Description: Identification of CD4 on human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes. Identification of CD8 on human T cells suppressor/cytotoxic expressing the 32,000 M.W. alpha subunit surface antigens.
Additional Text
Gene Name
CD4
Gene ID
920
Uniprot ID
P01730
Purification
Protein A/G Purified
Application Notes
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2 to 8C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Storage Note
Product should be stored at 4-8C. DO NOT FREEZE
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